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J. Cell Biol.
© The Rockefeller University Press
0021-9525/97/05/743/12 $2.00
Volume 137, Number 3, May 5, 1997 743-754

Expression of a P-selectin Ligand in Zona Pellucida of Porcine Oocytes and P-selectin on Acrosomal Membrane of Porcine Sperm Cells. Potential Implications for Their Involvement in Sperm-Egg Interactions

Jian-Guo Geng,* Thomas J. Raub,Dagger Carolyn A. Baker,§ Geri A. Sawada,Dagger Li Ma,par and Åke P. Elhammer§

* Cell Biology and Inflammation Research, Dagger  Drug Delivery Research, § Biochemistry and par  Molecular Biology Research, Pharmacia and Upjohn, Inc., Kalamazoo, Michigan 49001

The selectin family of cell adhesion molecules mediates initial leukocyte adhesion to vascular endothelial cells at sites of inflammation. O-glycan structural similarities between oligosaccharides from human leukocyte P-selectin glycoprotein ligand-1 (PSGL-1) and from zona pellucida glycoproteins of porcine oocytes indicate the possible existence of a P-selectin ligand in the zona pellucida. Here, using biochemical as well as morphological approaches, we demonstrate that a P-selectin ligand is expressed in the porcine zona pellucida. In addition, a search for a specific receptor for this ligand leads to the identification of P-selectin on the acrosomal membrane of porcine sperm cells. In vitro binding of porcine acrosome-reacted sperm cells to oocytes was found to be Ca2+ dependent and inhibitable with either P-selectin, P-selectin receptor-globulin, or leukocyte adhesion blocking antibodies against P-selectin and PSGL-1. Moreover, porcine sperm cells were found to be capable of binding to human promyeloid cell line HL-60. Taken together, our findings implicate a potential role for the oocyte P-selectin ligand and the sperm P-selectin in porcine sperm- egg interactions.


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