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* Cell Biology and Inflammation Research, The selectin family of cell adhesion molecules mediates initial leukocyte adhesion to vascular
endothelial cells at sites of inflammation. O-glycan
structural similarities between oligosaccharides from
human leukocyte P-selectin glycoprotein ligand-1
(PSGL-1) and from zona pellucida glycoproteins of
porcine oocytes indicate the possible existence of a
P-selectin ligand in the zona pellucida. Here, using biochemical as well as morphological approaches, we demonstrate that a P-selectin ligand is expressed in the porcine zona pellucida. In addition, a search for a specific
receptor for this ligand leads to the identification of
P-selectin on the acrosomal membrane of porcine
sperm cells. In vitro binding of porcine acrosome-reacted
sperm cells to oocytes was found to be Ca2+ dependent
and inhibitable with either P-selectin, P-selectin receptor-globulin, or leukocyte adhesion blocking antibodies
against P-selectin and PSGL-1. Moreover, porcine
sperm cells were found to be capable of binding to human promyeloid cell line HL-60. Taken together, our
findings implicate a potential role for the oocyte P-selectin ligand and the sperm P-selectin in porcine sperm- egg interactions.
Drug Delivery Research, § Biochemistry and
Molecular Biology Research,
Pharmacia and Upjohn, Inc., Kalamazoo, Michigan 49001
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