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© The Rockefeller University Press, 0021-9525/1997//743 $5.00
The Journal of Cell Biology, Volume 137, Number 3, , 1997 743-754

Article

Expression of a P-selectin Ligand in Zona Pellucida of Porcine Oocytes and P-selectin on Acrosomal Membrane of Porcine Sperm Cells. Potential Implications for Their Involvement in Sperm–Egg Interactions



Jian-Guo Geng*, Thomas J. Raub{ddagger}, Carolyn A. Baker§, Geri A. Sawada{ddagger}, Li Ma||, and Åke P. Elhammer§

* Cell Biology and Inflammation Research, {ddagger} Drug Delivery Research, § Biochemistry and || Molecular Biology Research, Pharmacia and Upjohn, Inc., Kalamazoo, Michigan 49001

The selectin family of cell adhesion molecules mediates initial leukocyte adhesion to vascular endothelial cells at sites of inflammation. O-glycan structural similarities between oligosaccharides from human leukocyte P-selectin glycoprotein ligand-1 (PSGL-1) and from zona pellucida glycoproteins of porcine oocytes indicate the possible existence of a P-selectin ligand in the zona pellucida. Here, using biochemical as well as morphological approaches, we demonstrate that a P-selectin ligand is expressed in the porcine zona pellucida. In addition, a search for a specific receptor for this ligand leads to the identification of P-selectin on the acrosomal membrane of porcine sperm cells. In vitro binding of porcine acrosome-reacted sperm cells to oocytes was found to be Ca2+ dependent and inhibitable with either P-selectin, P-selectin receptor–globulin, or leukocyte adhesion blocking antibodies against P-selectin and PSGL-1. Moreover, porcine sperm cells were found to be capable of binding to human promyeloid cell line HL-60. Taken together, our findings implicate a potential role for the oocyte P-selectin ligand and the sperm P-selectin in porcine sperm– egg interactions.

1. Abbreviations used in this paper: BCECF-AM, 2',7'-bis(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxylmethyl ester; PGSL-1, P-selectin glycoprotein ligand-1; PUVEC, porcine umbilical vein endothelial cell; Rg, receptor-globulin; TNF-{alpha}, tissue necrosis factor-{alpha}.

Please address all correspondence to Jian-Guo Geng, Cell Biology and Inflammation Research, 7239-267-307, Pharmacia and Upjohn, Inc., 301 Henrietta Street, Kalamazoo, MI 49001. Tel.: (616) 833-9677. Fax: (616) 833-9308. e-mail: jian-guo.geng{at}am.pnu.com


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