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* The Center for Blood Research and Department of Pathology, Harvard Medical School, Boston, Massachusetts 02115; and Tenascin is a large extracellular matrix molecule expressed at specific sites in the adult, including
immune system tissues such as the bone marrow, thymus, spleen, and T cell areas of lymph nodes. Tenascin
has been reported to have both adhesive and anti-adhesive effects in static assays. We report here that tenascin supports the tethering and rolling of lymphocytes
and lymphoblastic cell lines under flow conditions.
Binding was calcium dependent and was not inhibited
by treatment of lymphocytes with O-glycoprotease or a
panel of glycosidases including neuraminidase and heparitinase but was inhibited by treatment of cells with
proteinase K. Binding was to the fibrinogen-like terminal domain of tenascin as determined by antibody
blocking studies and binding to recombinant tenascin proteins. When compared to rolling of the same cell
type on E-selectin, rolling on tenascin was found to be
smoother at all shear stresses tested, suggesting that
cells formed a larger number of bonds on the tenascin
substrate than on the E-selectin substrate. When protein plating densities were adjusted to give similar profiles of cell detachment under increasing shears, the
density of tenascin was 8.5-fold greater than that of
E-selectin. Binding to tenascin was not dependent on
any molecules previously identified as tenascin receptors and is likely to involve a novel tenascin receptor on lymphocytes. We postulate that the ability of tenascin
to support lymphocyte rolling may reflect its ability to
support cell migration and that this interaction may be
used by lymphocytes migrating through secondary lymphoid organs.
Department of Cell Biology, Duke University Medical Center, Durham, North Carolina 27710
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