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Department of Biology, Yonsei University, Seoul, Korea 120-749
We have examined the distribution of four
mRNAs
-tubulin, 
-tubulin, flagellar calmodulin,
and Class I mRNAduring differentiation of Naegleria
gruberi amebas into flagellates by in situ hybridization. Three of the four mRNAs-tubulin, -tubulin, and
Class I mRNAbegan to be colocalized at the periphery of the cells as soon as transcription of the respective
genes was activated and before any microtubular structures were observable. At 70 min after the initiation of differentiation, these mRNAs were relocalized to the
base of the growing flagella, adjacent to the basal bodies and microtubule organizing center for the cytoskeletal microtubules. Within an additional 15 min, the
mRNAs were translocated to the posterior of the flagellated cells, and by the end of differentiation (120 min), very low levels of the mRNAs were observed.
Cytochalasin D inhibited stage-specific localization of
the mRNAs, demonstrating that RNA localization was
actin dependent. Since cytochalasin D also blocked differentiation, this raises the possibility that actin-dependent RNA movement is an essential process for differentiation.
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