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© The Rockefeller University Press, 0021-9525/1997//1001 $5.00
The Journal of Cell Biology, Volume 137, Number 5, , 1997 1001-1016

Article

Insertional Mutation of the Drosophila Nuclear Lamin Dm0 Gene Results in Defective Nuclear Envelopes, Clustering of Nuclear Pore Complexes, and Accumulation of Annulate Lamellae



Bettina Lenz-Böhme*, Jasmine Wismar*, Silke Fuchs*, Rita Reifegerste{ddagger}, Erich Buchner{ddagger}, Heinrich Betz*, and Bertram Schmitt*

* Max-Planck-Institut für Hirnforschung, Abteilung Neurochemie, D-60528 Frankfurt am Main, Germany; and {ddagger} Theodor-Bovery-Institut, Lehrstuhl für Genetik der Universität Würzburg, D-97074 Würzburg, Germany

Nuclear lamins are thought to play an important role in disassembly and reassembly of the nucleus during mitosis. Here, we describe a Drosophila lamin Dm0 mutant resulting from a P element insertion into the first intron of the Dm0 gene. Homozygous mutant animals showed a severe phenotype including retardation in development, reduced viability, sterility, and impaired locomotion. Immunocytochemical and ultrastructural analysis revealed that reduced lamin Dm0 expression caused an enrichment of nuclear pore complexes in cytoplasmic annulate lamellae and in nuclear envelope clusters. In several cells, particularly the densely packed somata of the central nervous system, defective nuclear envelopes were observed in addition. All aspects of the mutant phenotype were rescued upon P element-mediated germline transformation with a lamin Dm0 transgene. These data constitute the first genetic proof that lamins are essential for the structural organization of the cell nucleus.

1. Abbreviations used in this paper: LAP, lamina-associated proteins; NPC, nuclear pore complex; wt, wild type.

Please address all correspondence to B. Schmitt, Max-Planck-Institut für Hirnforschung, Abteilung Neurochemie, Deutschordenstrasse 46, D-60528 Frankfurt am Main, Germany. Tel.: (49) 69-96769-261; Fax: (49) 69-96769-441.

R. Reifegerste's current address is the Department of Biological Sciences, University of Southern California, Los Angeles, CA 90084-7340.


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