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© The Rockefeller University Press, 0021-9525/1997//1581 $5.00
The Journal of Cell Biology, Volume 137, Number 7, , 1997 1581-1588

Article

Human Bcl-2 Reverses Survival Defects in Yeast Lacking Superoxide Dismutase and Delays Death of Wild-Type Yeast



Valter D. Longo*, Lisa M. Ellerby{ddagger}, Dale E. Bredesen{ddagger},§, Joan S. Valentine*, and Edith B. Gralla*

* Department of Chemistry and Biochemistry, University of California at Los Angeles, Los Angeles, California 90095-1569; {ddagger} Program on Aging, The Burnham Institute, La Jolla, California 92037; and § Neuroscience Department, University of California at San Diego, La Jolla, California 92093

We expressed the human anti-apoptotic protein, Bcl-2, in Saccharomyces cerevisiae to investigate its effects on antioxidant protection and stationary phase survival. Yeast lacking copper-zinc superoxide dismutase (sod1{Delta}) show a profound defect in entry into and survival during stationary phase even under conditions optimal for survival of wild-type strains (incubation in water after stationary phase is reached). Expression of Bcl-2 in the sod1{Delta} strain caused a large improvement in viability at entry into stationary phase, as well as increased resistance to 100% oxygen and increased catalase activity. In addition, Bcl-2 expression reduced mutation frequency in both wild-type and sod1{Delta} strains. In another set of experiments, wild-type yeast incubated in expired minimal medium instead of water lost viability quickly; expression of Bcl-2 significantly delayed this stationary phase death. Our results demonstrate that Bcl-2 has activities in yeast that are similar to activities it is known to possess in mammalian cells: (a) stimulation of antioxidant protection and (b) delay of processes leading to cell death.

1. Abbreviations used in this paper: SDC, synthetic dextrose complete medium; SOD, superoxide dismutase; YPD, yeast extract/peptone/dextrose.

Please address all correspondence to E.B. Gralla, Department of Chemistry and Biochemistry, University of California at Los Angeles, Box 156905, Los Angeles, CA 90095-1569. Tel.: (310) 825-2807. Fax: (310) 206-7197. e-mail: egralla{at}chem.ucla.edu


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