Human Cytomegalovirus Immediate Early Interaction with Host Nuclear Structures: Definition of an Immediate Transcript Environment

doi:10.1083/jcb.138.1.5

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© The Rockefeller University Press, 0021-9525/1997//5 $5.00
The Journal of Cell Biology, Volume 138, Number 1, , 1997 5-16

Article

Human Cytomegalovirus Immediate Early Interaction with Host Nuclear Structures: Definition of an Immediate Transcript Environment

Alexander M. Ishov^*, Richard M. Stenberg, and Gerd G. Maul^*

^* The Wistar Institute, Philadelphia, Pennsylvania 19104; and Department of Microbiology and Immunology, Eastern Virginia Medical School, Norfolk, Virginia 23501

The development of an induced transcript environment was investigatedat the supramolecular level through comparative localizationof the human cytomegalovirus immediate early (IE) transcriptsand specific nuclear domains shortly after infection. Compactaggregates of IE transcripts form only adjacent to nucleardomain 10 (ND10), and the viral protein IE86 accumulates exclusivelyjuxtaposed to the subpopulation of ND10 with transcripts. Thestream of transcripts is funneled from ND10 into the spliceosomeassembly factor SC35 domain through the accumulation of IE86 protein, which recruits some components of the basal transcriptionmachinery. Concomitantly the IE72 protein binds to ND10 andlater disperses them. The domain containing the zinc fingerregion of IE72 is essential for this dispersal. Positional analysisof proteins IE86 and IE72, IE transcripts, ND10, the spliceosome assembly factor SC35, and basal transcription factors definesspatially and temporally an immediate transcript environment,the basic components of which exist in the cell before viralinfection, providing the structural environment for the virusto usurp.

Abbreviations used in this paper: IE, immediate early; HCMV, human cytomegalovirus; PML, promyelocytic leukemia protein; TBP, TATA-binding protein.

Please address all correspondence to Gerd G. Maul, The WistarInstitute, 3601 Spruce Street, Philadelphia, PA 19104. Tel.:(215) 898-3817; Fax: (215) 898-3868; E-mail: Maul{at}wista.wistar.upenn.edu

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