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© The Rockefeller University Press, 0021-9525/1997//395 $5.00
The Journal of Cell Biology, Volume 138, Number 2, , 1997 395-409

Article

KLP38B: A Mitotic Kinesin-related Protein That Binds PP1



Luke Alphey*, Louise Parker*, Gillian Hawcroft*, Yiquan Guo{ddagger}, Kim Kaiser{ddagger}, and Gareth Morgan*

* School of Biological Sciences, University of Manchester, Manchester M13 9PT; and {ddagger} Division of Molecular Genetics, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow G11 6NU, United Kingdom

We have identified a new member of the kinesin superfamily in Drosophila, KLP38B (kinesin-like protein at 38B). KLP38B was isolated through its two-hybrid interaction with the catalytic subunit of type 1 serine/threonine phosphoprotein phosphatase (PP1). We demonstrate that recombinant KLP38B and PP1 associate in vitro. This is the first demonstration of direct binding of a kinesin-related protein to a regulatory enzyme.

Though most closely related to the Unc-104 subfamily of kinesin-related proteins, KLP38B is expressed only in proliferating cells. KLP38B mutants show cell proliferation defects in many tissues. KLP38B is required for normal chromatin condensation as embryos from KLP38B mutant mothers have undercondensed chromatin at metaphase and anaphase. This is the first time that a kinesin-related protein has been shown to have such a role. Incomplete lethality of a strong KLP38B allele suggests partial redundancy with one or more additional kinesin-related proteins.

1. Abbreviations used in this paper: cdk, cyclin-dependent kinase; DAPI, 4',6-diamidino-2-phenylindole; KLP, kinesin-like protein; KRP, kinesin-related protein; ORF, open reading frame.

This work was principally supported by grant SP2290/0101 from the Cancer Research Campaign to L. Alphey, with additional funding from the Royal Society (to L. Alphey) and United Kingdom Biotechnology and Biological Sciences Research Council (to K. Kaiser). Confocal and fluorescence microscopes and semiautomated sequencing facilities were available through the generosity of the Wellcome Trust (grants 045183/Z/95/Z and 044327/Z/95/Z, respectively).

Please address all correspondence to Luke Alphey, School of Biological Sciences, University of Manchester, 2.205 Stopford, Oxford Road, Manchester M13 9PT, United Kingdom. Tel.: (44) 161-275-5111. Fax: (44) 161-275-5082. e-mail: Luke.Alphey{at}man.ac.uk


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