Cofilin Changes the Twist of F-Actin: Implications for Actin Filament Dynamics and Cellular Function

doi:10.1083/jcb.138.4.771

This Article

	Full Text
	Full Text (PDF, 713K)
	PPT slides of all figures
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JCB
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by McGough, A.
	Articles by Weeds, A.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by McGough, A.
	Articles by Weeds, A.


Social Bookmarking

	What's this?

© The Rockefeller University Press, 0021-9525/1997//771 $5.00
The Journal of Cell Biology, Volume 138, Number 4, , 1997 771-781

Article

Cofilin Changes the Twist of F-Actin: Implications for Actin Filament Dynamics and Cellular Function

Amy McGough^*, Brian Pope, Wah Chiu^*, and Alan Weeds

^* Verna and Marrs McLean Department of Biochemistry, Baylor College of Medicine, Houston, Texas 77030; and Medical Research Council Laboratory of Molecular Biology, Cambridge, CB2 2QH, United Kingdom

Cofilin is an actin depolymerizing protein found widely distributedin animals and plants. We have used electron cryomicroscopyand helical reconstruction to identify its binding site on actinfilaments. Cofilin binds filamentous (F)-actin cooperativelyby bridging two longitudinally associated actin subunits. The binding site is centered axially at subdomain 2 of thelower actin subunit and radially at the cleft between subdomains1 and 3 of the upper actin subunit. Our work has revealed atotally unexpected (and unique) property of cofilin, namely,its ability to change filament twist. As a consequence of thischange in twist, filaments decorated with cofilin have muchshorter ‘actin crossovers' ( $~$ 75% of those normally observedin F-actin structures). Although their binding sites are distinct, cofilin and phalloidin do not bind simultaneously to F-actin.This is the first demonstration of a protein that excludesanother actin-binding molecule by changing filament twist.Alteration of F-actin structure by cofilin/ADF appears to bea novel mechanism through which the actin cytoskeleton maybe regulated or remodeled.

Abbreviations used in this paper: F-actin, filamentous actin; G-actin, globular actin; l, layerline height; n, Bessel order; R, radial position of the first peak.

Please address all correspondence to Dr. Amy McGough, Vernaand Marrs McLean Department of Biochemistry, Baylor Collegeof Medicine, One Baylor Plaza, Houston, TX 77030. Tel.: (713)798-6989; Fax: (713) 796-9438.

CiteULike

Complore

Connotea

Del.icio.us Add to Digg

Digg

Facebook

Technorati

Twitter What's this?

This article has been cited by other articles:

Papalouka, V., Arvanitis, D. A., Vafiadaki, E., Mavroidis, M., Papadodima, S. A., Spiliopoulou, C. A., Kremastinos, D. T., Kranias, E. G., Sanoudou, D. (2009). Muscle Lim Protein Interacts with Cofilin 2 and Regulates F-Actin Dynamics in Cardiac and Skeletal Muscle. Mol. Cell. Biol. 29: 6046-6058 [Abstract] [Full Text]
Whiteman, I. T., Gervasio, O. L., Cullen, K. M., Guillemin, G. J., Jeong, E. V., Witting, P. K., Antao, S. T., Minamide, L. S., Bamburg, J. R., Goldsbury, C. (2009). Activated Actin-Depolymerizing Factor/Cofilin Sequesters Phosphorylated Microtubule-Associated Protein during the Assembly of Alzheimer-Like Neuritic Cytoskeletal Striations. J. Neurosci. 29: 12994-13005 [Abstract] [Full Text]
Bradley, W. D., Koleske, A. J. (2009). Regulation of cell migration and morphogenesis by Abl-family kinases: emerging mechanisms and physiological contexts. J. Cell Sci. 122: 3441-3454 [Abstract] [Full Text]
Kueh, H. Y., Mitchison, T. J. (2009). Structural Plasticity in Actin and Tubulin Polymer Dynamics. Science 325: 960-963 [Abstract] [Full Text]
Bryan, K. E., Rubenstein, P. A. (2009). Allele-specific Effects of Human Deafness {gamma}-Actin Mutations (DFNA20/26) on the Actin/Cofilin Interaction. J. Biol. Chem. 284: 18260-18269 [Abstract] [Full Text]
Skau, C. T., Neidt, E. M., Kovar, D. R. (2009). Role of Tropomyosin in Formin-mediated Contractile Ring Assembly in Fission Yeast. Mol. Biol. Cell 20: 2160-2173 [Abstract] [Full Text]
Stokasimov, E., McKane, M., Rubenstein, P. A. (2008). Role of Intermonomer Ionic Bridges in the Stabilization of the Actin Filament. J. Biol. Chem. 283: 34844-34854 [Abstract] [Full Text]
Paavilainen, V. O., Oksanen, E., Goldman, A., Lappalainen, P. (2008). Structure of the actin-depolymerizing factor homology domain in complex with actin. JCB 182: 51-59 [Abstract] [Full Text]
Kueh, H. Y., Charras, G. T., Mitchison, T. J., Brieher, W. M. (2008). Actin disassembly by cofilin, coronin, and Aip1 occurs in bursts and is inhibited by barbed-end cappers. JCB 182: 341-353 [Abstract] [Full Text]
Verdoni, A. M., Aoyama, N., Ikeda, A., Ikeda, S. (2008). Effect of destrin mutations on the gene expression profile in vivo. Physiol. Genomics 34: 9-21 [Abstract] [Full Text]
Le Clainche, C., Carlier, M.-F. (2008). Regulation of Actin Assembly Associated With Protrusion and Adhesion in Cell Migration. Physiol. Rev. 88: 489-513 [Abstract] [Full Text]
Gunning, P., O'neill, G., Hardeman, E. (2008). Tropomyosin-Based Regulation of the Actin Cytoskeleton in Time and Space. Physiol. Rev. 88: 1-35 [Abstract] [Full Text]
Mseka, T., Bamburg, J. R., Cramer, L. P. (2007). ADF/cofilin family proteins control formation of oriented actin-filament bundles in the cell body to trigger fibroblast polarization. J. Cell Sci. 120: 4332-4344 [Abstract] [Full Text]
Reisler, E., Egelman, E. H. (2007). Actin Structure and Function: What We Still Do Not Understand. J. Biol. Chem. 282: 36133-36137 [Full Text]
Yamashiro, S., Gimona, M., Ono, S. (2007). UNC-87, a calponin-related protein in C. elegans, antagonizes ADF/cofilin-mediated actin filament dynamics. J. Cell Sci. 120: 3022-3033 [Abstract] [Full Text]
Clark, M. G., Amberg, D. C. (2007). Biochemical and Genetic Analyses Provide Insight Into the Structural and Mechanistic Properties of Actin Filament Disassembly by the Aip1p Cofilin Complex in Saccharomyces cerevisiae. Genetics 176: 1527-1539 [Abstract] [Full Text]
Kamal, J. K. A., Benchaar, S. A., Takamoto, K., Reisler, E., Chance, M. R. (2007). Three-dimensional structure of cofilin bound to monomeric actin derived by structural mass spectrometry data. Proc. Natl. Acad. Sci. USA 104: 7910-7915 [Abstract] [Full Text]
Chaturvedi, P., Singh, A. P., Moniaux, N., Senapati, S., Chakraborty, S., Meza, J. L., Batra, S. K. (2007). MUC4 Mucin Potentiates Pancreatic Tumor Cell Proliferation, Survival, and Invasive Properties and Interferes with Its Interaction to Extracellular Matrix Proteins. Mol Cancer Res 5: 309-320 [Abstract] [Full Text]
Huang, Y., Burkhardt, J. K. (2007). T-cell-receptor-dependent actin regulatory mechanisms. J. Cell Sci. 120: 723-730 [Abstract] [Full Text]
Srivastava, J., Barber, D. L., Jacobson, M. P. (2007). Intracellular pH Sensors: Design Principles and Functional Significance. Physiology 22: 30-39 [Abstract] [Full Text]
Schubert, V., Dotti, C. G. (2007). Transmitting on actin: synaptic control of dendritic architecture. J. Cell Sci. 120: 205-212 [Abstract] [Full Text]
Moseley, J. B., Goode, B. L. (2006). The Yeast Actin Cytoskeleton: from Cellular Function to Biochemical Mechanism. Microbiol. Mol. Biol. Rev. 70: 605-645 [Abstract] [Full Text]
Pelikan Conchaudron, A., Didry, D., Le, K. H. D., Larquet, E., Boisset, N., Pantaloni, D., Carlier, M.-F. (2006). Analysis of Tetramethylrhodamine-labeled Actin Polymerization and Interaction with Actin Regulatory Proteins. J. Biol. Chem. 281: 24036-24047 [Abstract] [Full Text]
Okada, K., Ravi, H., Smith, E. M., Goode, B. L. (2006). Aip1 and Cofilin Promote Rapid Turnover of Yeast Actin Patches and Cables: A Coordinated Mechanism for Severing and Capping Filaments. Mol. Biol. Cell 17: 2855-2868 [Abstract] [Full Text]
Clark, M. G., Teply, J., Haarer, B. K., Viggiano, S. C., Sept, D., Amberg, D. C. (2006). A Genetic Dissection of Aip1p's Interactions Leads to a Model for Aip1p-Cofilin Cooperative Activities. Mol. Biol. Cell 17: 1971-1984 [Abstract] [Full Text]
Pandey, D., Goyal, P., Bamburg, J. R., Siess, W. (2006). Regulation of LIM-kinase 1 and cofilin in thrombin-stimulated platelets. Blood 107: 575-583 [Abstract] [Full Text]
Jang, D.-H., Han, J.-H., Lee, S.-H., Lee, Y.-S., Park, H., Lee, S.-H., Kim, H., Kaang, B.-K. (2005). Cofilin expression induces cofilin-actin rod formation and disrupts synaptic structure and function in Aplysia synapses. Proc. Natl. Acad. Sci. USA 102: 16072-16077 [Abstract] [Full Text]
Nakashima, K., Sato, N., Nakagaki, T., Abe, H., Ono, S., Obinata, T. (2005). Two Mouse Cofilin Isoforms, Muscle-Type (MCF) and Non-Muscle Type (NMCF), Interact with F-Actin with Different Efficiencies. J Biochem 138: 519-526 [Abstract] [Full Text]
Schuler, H., Mueller, A.-K., Matuschewski, K. (2005). A Plasmodium Actin-depolymerizing Factor That Binds Exclusively to Actin Monomers. Mol. Biol. Cell 16: 4013-4023 [Abstract] [Full Text]
Giganti, A., Plastino, J., Janji, B., Van Troys, M., Lentz, D., Ampe, C., Sykes, C., Friederich, E. (2005). Actin-filament cross-linking protein T-plastin increases Arp2/3-mediated actin-based movement. J. Cell Sci. 118: 1255-1265 [Abstract] [Full Text]
Bryan, K. E., Rubenstein, P. A. (2005). An Intermediate Form of ADP-F-actin. J. Biol. Chem. 280: 1696-1703 [Abstract] [Full Text]
DesMarais, V., Ghosh, M., Eddy, R., Condeelis, J. (2005). Cofilin takes the lead. J. Cell Sci. 118: 19-26 [Abstract] [Full Text]
Orlova, A., Shvetsov, A., Galkin, V. E., Kudryashov, D. S., Rubenstein, P. A., Egelman, E. H., Reisler, E. (2004). Actin-destabilizing factors disrupt filaments by means of a time reversal of polymerization. Proc. Natl. Acad. Sci. USA 101: 17664-17668 [Abstract] [Full Text]
Gehler, S., Shaw, A. E., Sarmiere, P. D., Bamburg, J. R., Letourneau, P. C. (2004). Brain-Derived Neurotrophic Factor Regulation of Retinal Growth Cone Filopodial Dynamics Is Mediated through Actin Depolymerizing Factor/Cofilin. J. Neurosci. 24: 10741-10749 [Abstract] [Full Text]
Ruegg, J., Holsboer, F., Turck, C., Rein, T. (2004). Cofilin 1 Is Revealed as an Inhibitor of Glucocorticoid Receptor by Analysis of Hormone-Resistant Cells. Mol. Cell. Biol. 24: 9371-9382 [Abstract] [Full Text]
Huff, T., Rosorius, O., Otto, A. M., Muller, C. S. G., Ballweber, E., Hannappel, E., Mannherz, H. G. (2004). Nuclear localisation of the G-actin sequestering peptide thymosin {beta}4. J. Cell Sci. 117: 5333-5341 [Abstract] [Full Text]
Mohri, K., Vorobiev, S., Fedorov, A. A., Almo, S. C., Ono, S. (2004). Identification of Functional Residues on Caenorhabditis elegans Actin-interacting Protein 1 (UNC-78) for Disassembly of Actin Depolymerizing Factor/Cofilin-bound Actin Filaments. J. Biol. Chem. 279: 31697-31707 [Abstract] [Full Text]
Grimme, S. J., Gao, X.-D., Martin, P. S., Tu, K., Tcheperegine, S. E., Corrado, K., Farewell, A. E., Orlean, P., Bi, E. (2004). Deficiencies in the Endoplasmic Reticulum (ER)-Membrane Protein Gab1p Perturb Transfer of Glycosylphosphatidylinositol to Proteins and Cause Perinuclear ER-associated Actin Bar Formation. Mol. Biol. Cell 15: 2758-2770 [Abstract] [Full Text]
Huang, S., Blanchoin, L., Chaudhry, F., Franklin-Tong, V. E., Staiger, C. J. (2004). A Gelsolin-like Protein from Papaver rhoeas Pollen (PrABP80) Stimulates Calcium-regulated Severing and Depolymerization of Actin Filaments. J. Biol. Chem. 279: 23364-23375 [Abstract] [Full Text]
Pope, B. J., Zierler-Gould, K. M., Kuhne, R., Weeds, A. G., Ball, L. J. (2004). Solution Structure of Human Cofilin: ACTIN BINDING, pH SENSITIVITY, AND RELATIONSHIP TO ACTIN-DEPOLYMERIZING FACTOR. J. Biol. Chem. 279: 4840-4848 [Abstract] [Full Text]
Kumar, N., Zhao, P., Tomar, A., Galea, C. A., Khurana, S. (2004). Association of Villin with Phosphatidylinositol 4,5-Bisphosphate Regulates the Actin Cytoskeleton. J. Biol. Chem. 279: 3096-3110 [Abstract] [Full Text]
Galkin, V. E., Orlova, A., VanLoock, M. S., Shvetsov, A., Reisler, E., Egelman, E. H. (2003). ADF/cofilin use an intrinsic mode of F-actin instability to disrupt actin filaments. JCB 163: 1057-1066 [Abstract] [Full Text]
Mohri, K., Ono, S. (2003). Actin filament disassembling activity of Caenorhabditis elegans actin-interacting protein 1 (UNC-78) is dependent on filament binding by a specific ADF/cofilin isoform. J. Cell Sci. 116: 4107-4118 [Abstract] [Full Text]
Voegtli, W. C., Madrona, A. Y., Wilson, D. K. (2003). The Structure of Aip1p, a WD Repeat Protein That Regulates Cofilin-mediated Actin Depolymerization. J. Biol. Chem. 278: 34373-34379 [Abstract] [Full Text]
Angelini, T. E., Liang, H., Wriggers, W., Wong, G. C. L. (2003). Like-charge attraction between polyelectrolytes induced by counterion charge density waves. Proc. Natl. Acad. Sci. USA 100: 8634-8637 [Abstract] [Full Text]
Ono, K., Parast, M., Alberico, C., Benian, G. M., Ono, S. (2003). Specific requirement for two ADF/cofilin isoforms in distinct actin-dependent processes in Caenorhabditis elegans. J. Cell Sci. 116: 2073-2085 [Abstract] [Full Text]
Vorobiev, S., Strokopytov, B., Drubin, D. G., Frieden, C., Ono, S., Condeelis, J., Rubenstein, P. A., Almo, S. C. (2003). The structure of nonvertebrate actin: Implications for the ATP hydrolytic mechanism. Proc. Natl. Acad. Sci. USA 100: 5760-5765 [Abstract] [Full Text]
Pendleton, A., Pope, B., Weeds, A., Koffer, A. (2003). Latrunculin B or ATP Depletion Induces Cofilin-dependent Translocation of Actin into Nuclei of Mast Cells. J. Biol. Chem. 278: 14394-14400 [Abstract] [Full Text]
Dos Remedios, C. G., Chhabra, D., Kekic, M., Dedova, I. V., Tsubakihara, M., Berry, D. A., Nosworthy, N. J. (2003). Actin Binding Proteins: Regulation of Cytoskeletal Microfilaments. Physiol. Rev. 83: 433-473 [Abstract] [Full Text]
Ashworth, S. L., Southgate, E. L., Sandoval, R. M., Meberg, P. J., Bamburg, J. R., Molitoris, B. A. (2003). ADF/cofilin mediates actin cytoskeletal alterations in LLC-PK cells during ATP depletion. Am. J. Physiol. Renal Physiol. 284: F852-F862 [Abstract] [Full Text]
Bryce, N. S., Schevzov, G., Ferguson, V., Percival, J. M., Lin, J. J.-C., Matsumura, F., Bamburg, J. R., Jeffrey, P. L., Hardeman, E. C., Gunning, P., Weinberger, R. P. (2003). Specification of Actin Filament Function and Molecular Composition by Tropomyosin Isoforms. Mol. Biol. Cell 14: 1002-1016 [Abstract] [Full Text]
Dawson, J. F., Sablin, E. P., Spudich, J. A., Fletterick, R. J. (2003). Structure of an F-actin Trimer Disrupted by Gelsolin and Implications for the Mechanism of Severing. J. Biol. Chem. 278: 1229-1238 [Abstract] [Full Text]
Samstag, Y., Eibert, S. M., Klemke, M., Wabnitz, G. H. (2003). Actin cytoskeletal dynamics in T lymphocyte activation and migration. J. Leukoc. Biol. 73: 30-48 [Abstract] [Full Text]
Chen, C. Y.-h., Cheung, A. Y., Wu, H.-m. (2003). Actin-Depolymerizing Factor Mediates Rac/Rop GTPase-Regulated Pollen Tube Growth. Plant Cell 15: 237-249 [Abstract] [Full Text]
Chen, C. Y., Wong, E. I., Vidali, L., Estavillo, A., Hepler, P. K., Wu, H.-m., Cheung, A. Y. (2002). The Regulation of Actin Organization by Actin-Depolymerizing Factor in Elongating Pollen Tubes. Plant Cell 14: 2175-2190 [Abstract] [Full Text]
Nyakern-Meazza, M., Narayan, K., Schutt, C. E., Lindberg, U. (2002). Tropomyosin and Gelsolin Cooperate in Controlling the Microfilament System. J. Biol. Chem. 277: 28774-28779 [Abstract] [Full Text]
Moriyama, K., Yahara, I. (2002). Human CAP1 is a key factor in the recycling of cofilin and actin for rapid actin turnover. J. Cell Sci. 115: 1591-1601 [Abstract] [Full Text]
Ono, S., Ono, K. (2002). Tropomyosin inhibits ADF/cofilin-dependent actin filament dynamics. JCB 156: 1065-1076 [Abstract] [Full Text]
Woodring, P. J., Litwack, E. D., O'Leary, D. D.M., Lucero, G. R., Wang, J. Y.J., Hunter, T. (2002). Modulation of the F-actin cytoskeleton by c-Abl tyrosine kinase in cell spreading and neurite extension. JCB 156: 879-892 [Abstract] [Full Text]
Maselli, A. G., Davis, R., Furukawa, R., Fechheimer, M. (2002). Formation of Hirano bodies in Dictyostelium and mammalian cells induced by expression of a modified form of an actin-crosslinking protein. J. Cell Sci. 115: 1939-1949 [Abstract] [Full Text]
Rueckschloss, U, Isenberg, G (2001). Cytochalasin D reduces Ca2+ currents via cofilin-activated depolymerization of F-actin in guinea-pig cardiomyocytes. J. Physiol. 537: 363-370 [Abstract] [Full Text]
Dong, C.-H., Xia, G.-X., Hong, Y., Ramachandran, S., Kost, B., Chua, N.-H. (2001). ADF Proteins Are Involved in the Control of Flowering and Regulate F-Actin Organization, Cell Expansion, and Organ Growth in Arabidopsis. Plant Cell 13: 1333-1346 [Abstract] [Full Text]
Ashworth, S. L., Sandoval, R. M., Hosford, M., Bamburg, J. R., Molitoris, B. A. (2001). Ischemic injury induces ADF relocalization to the apical domain of rat proximal tubule cells. Am. J. Physiol. Renal Physiol. 280: F886-F894 [Abstract] [Full Text]
Galkin, V. E., Orlova, A., Lukoyanova, N., Wriggers, W., Egelman, E. H. (2001). Actin Depolymerizing Factor Stabilizes an Existing State of F-Actin and Can Change the Tilt of F-Actin Subunits. JCB 153: 75-86 [Abstract] [Full Text]
Ono, S. (2001). The Caenorhabditis elegans unc-78 Gene Encodes a Homologue of Actin-Interacting Protein 1 Required for Organized Assembly of Muscle Actin Filaments. JCB 152: 1313-1320 [Abstract] [Full Text]
Nogales, E., Grigorieff, N. (2001). Molecular Machines: Putting the Pieces Together. JCB 152: f1-f10 [Full Text]
Zebda, N., Bernard, O., Bailly, M., Welti, S., Lawrence, D. S., Condeelis, J. S. (2000). Phosphorylation of Adf/Cofilin Abolishes Egf-Induced Actin Nucleation at the Leading Edge and Subsequent Lamellipod Extension. JCB 151: 1119-1128 [Abstract] [Full Text]
Ojima, K., Lin, Z.X., Bang, M.-L., Holtzer, S., Matsuda, R., Labeit, S., Sweeney, H.L., Holtzer, H. (2000). Distinct Families of Z-Line Targeting Modules in the Cooh-Terminal Region of Nebulin. JCB 150: 553-566 [Abstract] [Full Text]
Southwick, F. S. (2000). Gelsolin and ADF/cofilin enhance the actin dynamics of motile cells. Proc. Natl. Acad. Sci. USA 97: 6936-6938 [Full Text]
Meberg, P. J., Bamburg, J. R. (2000). Increase in Neurite Outgrowth Mediated by Overexpression of Actin Depolymerizing Factor. J. Neurosci. 20: 2459-2469 [Abstract] [Full Text]
Chan, A. Y., Bailly, M., Zebda, N., Segall, J. E., Condeelis, J. S. (2000). Role of Cofilin in Epidermal Growth Factor-Stimulated Actin Polymerization and Lamellipod Protrusion. JCB 148: 531-542 [Abstract] [Full Text]
Carlier, M.-F., Ressad, F., Pantaloni, D. (1999). Control of Actin Dynamics in Cell Motility. ROLE OF ADF/COFILIN. J. Biol. Chem. 274: 33827-33830 [Full Text]
Renoult, C., Ternent, D., Maciver, S. K., Fattoum, A., Astier, C., Benyamin, Y., Roustan, C. (1999). The Identification of a Second Cofilin Binding Site on Actin Suggests a Novel, Intercalated Arrangement of F-actin Binding. J. Biol. Chem. 274: 28893-28899 [Abstract] [Full Text]
Ressad, F., Didry, D., Egile, C., Pantaloni, D., Carlier, M.-F. (1999). Control of Actin Filament Length and Turnover by Actin Depolymerizing Factor (ADF/Cofilin) in the Presence of Capping Proteins and ARP2/3 Complex. J. Biol. Chem. 274: 20970-20976 [Abstract] [Full Text]
Rodal, A. A., Tetreault, J. W., Lappalainen, P., Drubin, D. G., Amberg, D. C. (1999). Aip1p Interacts with Cofilin to Disassemble Actin Filaments. JCB 145: 1251-1264 [Abstract] [Full Text]
Blanchoin, L., Pollard, T. D. (1999). Mechanism of Interaction of Acanthamoeba Actophorin (ADF/Cofilin) with Actin Filaments. J. Biol. Chem. 274: 15538-15546 [Abstract] [Full Text]
Ono, S., Baillie, D. L., Benian, G. M. (1999). UNC-60B, an ADF/Cofilin Family Protein, Is Required for Proper Assembly of Actin into Myofibrils in Caenorhabditis elegans Body Wall Muscle. JCB 145: 491-502 [Abstract] [Full Text]
Schwartz, N., Hosford, M., Sandoval, R. M., Wagner, M. C., Atkinson, S. J., Bamburg, J., Molitoris, B. A. (1999). Ischemia activates actin depolymerizing factor: role in proximal tubule microvillar actin alterations. Am. J. Physiol. Renal Physiol. 276: F544-F551 [Abstract] [Full Text]
Brault, V, Reedy, M., Sauder, U, Kammerer, R., Aebi, U, Schoenenberger, C (1999). Substitution of flight muscle-specific actin by human (beta)-cytoplasmic actin in the indirect flight muscle of Drosophila. J. Cell Sci. 112: 3627-3639 [Abstract]
Okada, K, Obinata, T, Abe, H (1999). XAIP1: a Xenopus homologue of yeast actin interacting protein 1 (AIP1), which induces disassembly of actin filaments cooperatively with ADF/cofilin family proteins. J. Cell Sci. 112: 1553-1565 [Abstract]
Gonsior, S., Platz, S, Buchmeier, S, Scheer, U, Jockusch, B., Hinssen, H (1999). Conformational difference between nuclear and cytoplasmic actin as detected by a monoclonal antibody. J. Cell Sci. 112: 797-809 [Abstract]
Brault, V., Sauder, U., Reedy, M. C., Aebi, U., Schoenenberger, C.-A. (1999). Differential Epitope Tagging of Actin in Transformed Drosophila Produces Distinct Effects on Myofibril Assembly and Function of the Indirect Flight Muscle. Mol. Biol. Cell 10: 135-149 [Abstract] [Full Text]
Didry, D., Carlier, M.-F., Pantaloni, D. (1998). Synergy between Actin Depolymerizing Factor/Cofilin and Profilin in Increasing Actin Filament Turnover. J. Biol. Chem. 273: 25602-25611 [Abstract] [Full Text]
Blanchoin, L., Pollard, T. D. (1998). Interaction of Actin Monomers with Acanthamoeba Actophorin (ADF/Cofilin) and Profilin. J. Biol. Chem. 273: 25106-25111 [Abstract] [Full Text]
Ressad, F., Didry, D., Xia, G.-X., Hong, Y., Chua, N.-H., Pantaloni, D., Carlier, M.-F. (1998). Kinetic Analysis of the Interaction of Actin-depolymerizing Factor (ADF)/Cofilin with G- and F-Actins. COMPARISON OF PLANT AND HUMAN ADFs AND EFFECT OF PHOSPHORYLATION. J. Biol. Chem. 273: 20894-20902 [Abstract] [Full Text]
Lappalainen, P., Kessels, M. M., Cope, M. J. T.V., Drubin, D. G. (1998). The ADF Homology (ADF-H) Domain: A Highly Exploited Actin-binding Module. Mol. Biol. Cell 9: 1951-1959 [Full Text]
Ono, S., Benian, G. M. (1998). Two Caenorhabditis elegans Actin Depolymerizing Factor/Cofilin Proteins, Encoded by the unc-60 Gene, Differentially Regulate Actin Filament Dynamics. J. Biol. Chem. 273: 3778-3783 [Abstract] [Full Text]
Van Troys, M., Dewitte, D., Verschelde, J.-L., Goethals, M., Vandekerckhove, J., Ampe, C. (1997). Analogous F-actin Binding by Cofilin and Gelsolin Segment 2�Substantiates Their Structural Relationship. J. Biol. Chem. 272: 32750-32758 [Abstract] [Full Text]
Ono, S., McGough, A., Pope, B. J., Tolbert, V. T., Bui, A., Pohl, J., Benian, G. M., Gernert, K. M., Weeds, A. G. (2001). The C-terminal Tail of UNC-60B (Actin Depolymerizing Factor/Cofilin) Is Critical for Maintaining Its Stable Association with F-actin and Is Implicated in the Second Actin-binding Site. J. Biol. Chem. 276: 5952-5958 [Abstract] [Full Text]
Pfannstiel, J., Cyrklaff, M., Habermann, A., Stoeva, S., Griffiths, G., Shoeman, R., Faulstich, H. (2001). Human Cofilin Forms Oligomers Exhibiting Actin Bundling Activity. J. Biol. Chem. 276: 49476-49484 [Abstract] [Full Text]
Woodring, P. J., Litwack, E. D., O'Leary, D. D.M., Lucero, G. R., Wang, J. Y.J., Hunter, T. (2002). Modulation of the F-actin cytoskeleton by c-Abl tyrosine kinase in cell spreading and neurite extension. JCB 156: 879-892 [Abstract] [Full Text]
Vartiainen, M. K., Mustonen, T., Mattila, P. K., Ojala, P. J., Thesleff, I., Partanen, J., Lappalainen, P. (2002). The Three Mouse Actin-depolymerizing Factor/Cofilins Evolved to Fulfill Cell-Type-specific Requirements for Actin Dynamics. Mol. Biol. Cell 13: 183-194 [Abstract] [Full Text]