J. Cell Biol.
© The Rockefeller University Press
0021-9525/97/09/1379/16 $2.00
Volume 138, Number 6, September 22, 1997 1379-1394

Caspase Cleavage of Keratin 18 and Reorganization of Intermediate Filaments during Epithelial Cell Apoptosis

Carlos Caulín, Guy S. Salvesen, and Robert G. Oshima

The Burnham Institute (formerly the La Jolla Cancer Research Foundation), La Jolla, California 92037

Keratins 8 (K8) and 18 (K18) are major components of intermediate filaments (IFs) of simple epithelial cells and tumors derived from such cells. Structural cell changes during apoptosis are mediated by proteases of the caspase family. During apoptosis, K18 IFs reorganize into granular structures enriched for K18 phosphorylated on serine 53. K18, but not K8, generates a proteolytic fragment during drug- and UV light-induced apoptosis; this fragment comigrates with K18 cleaved in vitro by caspase-6, -3, and -7. K18 is cleaved by caspase-6 into NH₂-terminal, 26-kD and COOH-terminal, 22-kD fragments; caspase-3 and -7 additionally cleave the 22-kD fragment into a 19-kD fragment. The cleavage site common for the three caspases was the sequence VEVD/A, located in the conserved L1-2 linker region of K18. The additional site for caspases-3 and -7 that is not cleaved efficiently by caspase-6 is located in the COOH-terminal tail domain of K18. Expression of K18 with alanine instead of serine at position 53 demonstrated that cleavage during apoptosis does not require phosphorylation of serine 53. However, K18 with a glutamate instead of aspartate at position 238 was resistant to proteolysis during apoptosis. Furthermore, this cleavage site mutant appears to cause keratin filament reorganization in stably transfected clones. The identification of the L1-2 caspase cleavage site, and the conservation of the same or very similar sites in multiple other intermediate filament proteins, suggests that the processing of IFs during apoptosis may be initiated by a similar caspase cleavage.

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• Jain, J. K., Li, A., Nucatola, D. L., Minoo, P., Felix, J. C. (2005). Nonoxynol-9 Induces Apoptosis of Endometrial Explants by Both Caspase-Dependent and -Independent Apoptotic Pathways. Biol. Reprod. 73: 382-388 [Abstract] [Full Text]  
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• Li, A., Felix, J.C., Hao, J., Minoo, P., Jain, J.K. (2005). Menstrual-like breakdown and apoptosis in human endometrial explants. Hum Reprod 20: 1709-1719 [Abstract] [Full Text]  
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• Bao, S., Wang, Y., Sweeney, P., Chaudhuri, A., Doseff, A. I., Marsh, C. B., Knoell, D. L. (2005). Keratinocyte growth factor induces Akt kinase activity and inhibits Fas-mediated apoptosis in A549 lung epithelial cells. Am. J. Physiol. Lung Cell. Mol. Physiol. 288: L36-L42 [Abstract] [Full Text]  
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• Jarry, A., Charrier, L., Bou-Hanna, C., Devilder, M.-C., Crussaire, V., Denis, M. G., Vallette, G., Laboisse, C. L. (2004). Position in Cell Cycle Controls the Sensitivity of Colon Cancer Cells to Nitric Oxide-Dependent Programmed Cell Death. Cancer Res. 64: 4227-4234 [Abstract] [Full Text]  
• Sun, S.-Y., Hail, N. Jr, Lotan, R. (2004). Apoptosis as a Novel Target for Cancer Chemoprevention. JNCI J Natl Cancer Inst 96: 662-672 [Abstract] [Full Text]  
• Kramer, G., Erdal, H., Mertens, H. J. M. M., Nap, M., Mauermann, J., Steiner, G., Marberger, M., Biven, K., Shoshan, M. C., Linder, S. (2004). Differentiation between Cell Death Modes Using Measurements of Different Soluble Forms of Extracellular Cytokeratin 18. Cancer Res. 64: 1751-1756 [Abstract] [Full Text]  
• Dinsdale, D., Lee, J. C., Dewson, G., Cohen, G. M., Peter, M. E. (2004). Intermediate Filaments Control the Intracellular Distribution of Caspases During Apoptosis. Am. J. Pathol. 164: 395-407 [Abstract] [Full Text]  
• Bruewer, M., Luegering, A., Kucharzik, T., Parkos, C. A., Madara, J. L., Hopkins, A. M., Nusrat, A. (2003). Proinflammatory Cytokines Disrupt Epithelial Barrier Function by Apoptosis-Independent Mechanisms. J. Immunol. 171: 6164-6172 [Abstract] [Full Text]  
• Ferrandina, G., Ranelletti, F. O., Legge, F., Lauriola, L., Salutari, V., Gessi, M., Testa, A. C., Werner, U., Navarra, P., Tringali, G., Battaglia, A., Scambia, G. (2003). Celecoxib Modulates the Expression of Cyclooxygenase-2, Ki67, Apoptosis-Related Marker, and Microvessel Density in Human Cervical Cancer: A Pilot Study. Clin. Cancer Res. 9: 4324-4331 [Abstract] [Full Text]  
• Kazerounian, S., Aho, S. (2003). Characterization of Periphilin, a Widespread, Highly Insoluble Nuclear Protein and Potential Constituent of the Keratinocyte Cornified Envelope. J. Biol. Chem. 278: 36707-36717 [Abstract] [Full Text]  
• Al-Molawi, N., Beardmore, V. A., Carter, M. J., Kass, G. E. N., Roberts, L. O. (2003). Caspase-mediated cleavage of the feline calicivirus capsid protein. J. Gen. Virol. 84: 1237-1244 [Abstract] [Full Text]  
• Maxwell, S. A., Rivera, A. (2003). Proline Oxidase Induces Apoptosis in Tumor Cells, and Its Expression Is Frequently Absent or Reduced in Renal Carcinomas. J. Biol. Chem. 278: 9784-9789 [Abstract] [Full Text]  
• Chen, F., Chang, R., Trivedi, M., Capetanaki, Y., Cryns, V. L. (2003). Caspase Proteolysis of Desmin Produces a Dominant-negative Inhibitor of Intermediate Filaments and Promotes Apoptosis. J. Biol. Chem. 278: 6848-6853 [Abstract] [Full Text]  
• Kirshner, J., Chen, C.-J., Liu, P., Huang, J., Shively, J. E. (2003). CEACAM1-4S, a cell-cell adhesion molecule, mediates apoptosis and reverts mammary carcinoma cells to a normal morphogenic phenotype in a 3D culture. Proc. Natl. Acad. Sci. USA 100: 521-526 [Abstract] [Full Text]  
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• Thorburn, J., Bender, L. M., Morgan, M. J., Thorburn, A. (2003). Caspase- and Serine Protease-dependent Apoptosis by the Death Domain of FADD in Normal Epithelial Cells. Mol. Biol. Cell 14: 67-77 [Abstract] [Full Text]  
• Tesfaigzi, Y., Fischer, M. J., Daheshia, M., Green, F. H. Y., De Sanctis, G. T., Wilder, J. A. (2002). Bax is Crucial for IFN-{gamma}-Induced Resolution of Allergen- Induced Mucus Cell Metaplasia. J. Immunol. 169: 5919-5925 [Abstract] [Full Text]  
• Lee, J. C., Schickling, O., Stegh, A. H., Oshima, R. G., Dinsdale, D., Cohen, G. M., Peter, M. E. (2002). DEDD regulates degradation of intermediate filaments during apoptosis. JCB 158: 1051-1066 [Abstract] [Full Text]  
• Bucchieri, F., Puddicombe, S. M., Lordan, J. L., Richter, A., Buchanan, D., Wilson, S. J., Ward, J., Zummo, G., Howarth, P. H., Djukanovic, R., Holgate, S. T., Davies, D. E. (2002). Asthmatic Bronchial Epithelium Is More Susceptible to Oxidant-Induced Apoptosis. Am. J. Respir. Cell Mol. Bio. 27: 179-185 [Abstract] [Full Text]  
• Chin, A. C., Teoh, D. A., Scott, K. G.-E., Meddings, J. B., Macnaughton, W. K., Buret, A. G. (2002). Strain-Dependent Induction of Enterocyte Apoptosis by Giardia lamblia Disrupts Epithelial Barrier Function in a Caspase-3-Dependent Manner. Infect. Immun. 70: 3673-3680 [Abstract] [Full Text]  
• Ditzel, H. J., Strik, M. C. M., Larsen, M. K., Willis, A. C., Waseem, A., Kejling, K., Jensenius, J. C. (2002). Cancer-associated Cleavage of Cytokeratin 8/18 Heterotypic Complexes Exposes a Neoepitope in Human Adenocarcinomas. J. Biol. Chem. 277: 21712-21722 [Abstract] [Full Text]  
• Gown, A. M., Willingham, M. C. (2002). Improved Detection of Apoptotic Cells in Archival Paraffin Sections: Immunohistochemistry Using Antibodies to Cleaved Caspase 3. J. Histochem. Cytochem. 50: 449-454 [Abstract] [Full Text]  
• Charrier, L., Jarry, A., Toquet, C., Bou-Hanna, C., Chedorge, M., Denis, M., Vallette, G., Laboisse, C. L. (2002). Growth Phase-dependent Expression of ICAD-L/DFF45 Modulates the Pattern of Apoptosis in Human Colonic Cancer Cells. Cancer Res. 62: 2169-2174 [Abstract] [Full Text]  
• He, T., Stepulak, A., Holmstrom, T. H., Omary, M. B., Eriksson, J. E. (2002). The Intermediate Filament Protein Keratin 8 Is a Novel Cytoplasmic Substrate for c-Jun N-terminal Kinase. J. Biol. Chem. 277: 10767-10774 [Abstract] [Full Text]  
• Lavastre, V., Pelletier, M., Saller, R., Hostanska, K., Girard, D. (2002). Mechanisms Involved in Spontaneous and Viscum album Agglutinin-I-Induced Human Neutrophil Apoptosis: Viscum album Agglutinin-I Accelerates the Loss of Antiapoptotic Mcl-1 Expression and the Degradation of Cytoskeletal Paxillin and Vimentin Proteins Via Caspases. J. Immunol. 168: 1419-1427 [Abstract] [Full Text]  
• Coulter, K. R., Doseff, A., Sweeney, P., Wang, Y., Marsh, C. B., Wewers, M. D., Knoell, D. L. (2002). Opposing Effect by Cytokines on Fas-Mediated Apoptosis in A549 Lung Epithelial Cells. Am. J. Respir. Cell Mol. Bio. 26: 58-66 [Abstract] [Full Text]  
• Abreu, M. T., Arnold, E. T., Chow, J. Y. C., Barrett, K. E. (2001). Phosphatidylinositol 3-Kinase-dependent Pathways Oppose Fas-induced Apoptosis and Limit Chloride Secretion in Human Intestinal Epithelial Cells. IMPLICATIONS FOR INFLAMMATORY DIARRHEAL STATES. J. Biol. Chem. 276: 47563-47574 [Abstract] [Full Text]  
• Kim, J.-M., Ghosh, S. R., Weil, A. C. P., Zirkin, B. R. (2001). Caspase-3 and Caspase-Activated Deoxyribonuclease Are Associated with Testicular Germ Cell Apoptosis Resulting from Reduced Intratesticular Testosterone. Endocrinology 142: 3809-3816 [Abstract] [Full Text]  
• Ferrandina, G., Ranelletti, F. O., Larocca, L. M., Maggiano, N., Fruscella, E., Legge, F., Santeusanio, G., Bombonati, A., Mancuso, S., Scambia, G. (2001). Tamoxifen Modulates the Expression of Ki67, Apoptosis, and Microvessel Density in Cervical Cancer. Clin. Cancer Res. 7: 2656-2661 [Abstract] [Full Text]  
• Gilbert, S., Loranger, A., Daigle, N., Marceau, N. (2001). Simple epithelium keratins 8 and 18 provide resistance to Fas-mediated apoptosis. The protection occurs through a receptor-targeting modulation. JCB 154: 763-774 [Abstract] [Full Text]  
• Galande, S., Dickinson, L. A., Mian, I. S., Sikorska, M., Kohwi-Shigematsu, T. (2001). SATB1 Cleavage by Caspase 6 Disrupts PDZ Domain-Mediated Dimerization, Causing Detachment from Chromatin Early in T-Cell Apoptosis. Mol. Cell. Biol. 21: 5591-5604 [Abstract] [Full Text]  
• Nyormoi, O., Wang, Z., Doan, D., Ruiz, M., McConkey, D., Bar-Eli, M. (2001). Transcription Factor AP-2{alpha} Is Preferentially Cleaved by Caspase 6 and Degraded by Proteasome during Tumor Necrosis Factor Alpha-Induced Apoptosis in Breast Cancer Cells. Mol. Cell. Biol. 21: 4856-4867 [Abstract] [Full Text]  
• Barrett, K. L., Willingham, J. M., Garvin, A. J., Willingham, M. C. (2001). Advances in Cytochemical Methods for Detection of Apoptosis. J. Histochem. Cytochem. 49: 821-832 [Abstract] [Full Text]  
• Mehes, G., Witt, A., Kubista, E., Ambros, P. F. (2001). Circulating Breast Cancer Cells Are Frequently Apoptotic. Am. J. Pathol. 159: 17-20 [Abstract] [Full Text]  
• Lane, J. D., Vergnolle, M. A.S., Woodman, P. G., Allan, V. J. (2001). Apoptotic Cleavage of Cytoplasmic Dynein Intermediate Chain and p150GluedStops Dynein-dependent Membrane Motility. JCB 153: 1415-1426 [Abstract] [Full Text]  
• Mandic, A., Viktorsson, K., Molin, M., Akusjärvi, G., Eguchi, H., Hayashi, S.-I., Toi, M., Hansson, J., Linder, S., Shoshan, M. C. (2001). Cisplatin Induces the Proapoptotic Conformation of Bak in a {Delta}MEKK1-Dependent Manner. Mol. Cell. Biol. 21: 3684-3691 [Abstract] [Full Text]  
• Reichelt, J., Bussow, H., Grund, C., Magin, T. M. (2001). Formation of a Normal Epidermis Supported by Increased Stability of Keratins 5 and 14 in Keratin 10 Null Mice. Mol. Biol. Cell 12: 1557-1568 [Abstract] [Full Text]  
• Piazza, G. A., Thompson, W. J., Pamukcu, R., Alila, H. W., Whitehead, C. M., Liu, L., Fetter, J. R., Gresh, W. E. Jr., Klein-Szanto, A. J., Farnell, D. R., Eto, I., Grubbs, C. J. (2001). Exisulind, a Novel Proapoptotic Drug, Inhibits Rat Urinary Bladder Tumorigenesis. Cancer Res. 61: 3961-3968 [Abstract] [Full Text]  
• Stegh, A. H., Herrmann, H., Lampel, S., Weisenberger, D., Andrä, K., Seper, M., Wiche, G., Krammer, P. H., Peter, M. E. (2000). Identification of the Cytolinker Plectin as a Major Early In Vivo Substrate for Caspase 8 during CD95- and Tumor Necrosis Factor Receptor-Mediated Apoptosis. Mol. Cell. Biol. 20: 5665-5679 [Abstract] [Full Text]  
• Ku, N.-O., Omary, M. B. (2000). Keratins Turn Over by Ubiquitination in a Phosphorylation-modulated Fashion. JCB 149: 547-552 [Abstract] [Full Text]  
• Caulin, C., Ware, C. F., Magin, T. M., Oshima, R. G. (2000). Keratin-dependent, Epithelial Resistance to Tumor Necrosis Factor-induced Apoptosis. JCB 149: 17-22 [Abstract] [Full Text]  
• Subauste, M. C., Von Herrath, M., Benard, V., Chamberlain, C. E., Chuang, T.-H., Chu, K., Bokoch, G. M., Hahn, K. M. (2000). Rho Family Proteins Modulate Rapid Apoptosis Induced by Cytotoxic T Lymphocytes and Fas. J. Biol. Chem. 275: 9725-9733 [Abstract] [Full Text]  
• MacFarlane, M., Merrison, W., Dinsdale, D., Cohen, G. M. (2000). Active Caspases and Cleaved Cytokeratins Are Sequestered into Cytoplasmic Inclusions in TRAIL-induced Apoptosis. JCB 148: 1239-1254 [Abstract] [Full Text]  
• McCole, D. F., Eckmann, L., Laurent, F., Kagnoff, M. F. (2000). Intestinal Epithelial Cell Apoptosis following Cryptosporidium parvum Infection. Infect. Immun. 68: 1710-1713 [Abstract] [Full Text]  
• Marchi, E., Liu, W., Broaddus, V. C. (2000). Mesothelial cell apoptosis is confirmed in vivo by morphological change in cytokeratin distribution. Am. J. Physiol. Lung Cell. Mol. Physiol. 278: L528-L535 [Abstract] [Full Text]  
• Saraste, A., Pulkki, K. (2000). Morphologic and biochemical hallmarks of apoptosis. Cardiovasc Res 45: 528-537 [Abstract] [Full Text]  
• Bursch, W, Hochegger, K, Torok, L, Marian, B, Ellinger, A, Hermann, R. (2000). Autophagic and apoptotic types of programmed cell death exhibit different fates of cytoskeletal filaments. J. Cell Sci. 113: 1189-1198 [Abstract]  
• Farina, F., Cappello, F., Todaro, M., Bucchieri, F., Peri, G., Zummo, G., Stassi, G. (2000). Involvement of Caspase-3 and GD3 Ganglioside in Ceramide-induced Apoptosis in Farber Disease. J. Histochem. Cytochem. 48: 57-62 [Abstract] [Full Text]  
• Ku, N.-O., Zhou, X., Toivola, D. M., Omary, M. B. (1999). The cytoskeleton of digestive epithelia in health and disease. Am. J. Physiol. Gastrointest. Liver Physiol. 277: G1108-G1137 [Abstract] [Full Text]  
• Ebert, A. D., Wechselberger, C., Frank, S., Wallace-Jones, B., Seno, M., Martinez-Lacaci, I., Bianco, C., De Santis, M., Weitzel, H. K., Salomon, D. S. (1999). Cripto-1 Induces Phosphatidylinositol 3'-Kinase-dependent Phosphorylation of AKT and Glycogen Synthase Kinase 3{beta} in Human Cervical Carcinoma Cells. Cancer Res. 59: 4502-4505 [Abstract] [Full Text]  
• Stabler, S. M., Ostrowski, L. L., Janicki, S. M., Monteiro, M. J. (1999). A Myristoylated Calcium-binding Protein that Preferentially Interacts with the Alzheimer's Disease Presenilin 2 Protein. JCB 145: 1277-1292 [Abstract] [Full Text]  
• Ku, N.-O., Michie, S. A., Soetikno, R. M., Resurreccion, E. Z., Broome, R. L., Omary, M. B. (1998). Mutation of a Major Keratin Phosphorylation Site Predisposes to Hepatotoxic Injury in Transgenic Mice. JCB 143: 2023-2032 [Abstract] [Full Text]  
• Fradette, J., Germain, L., Seshaiah, P., Coulombe, P. A. (1998). The Type I Keratin 19 Possesses Distinct and Context-dependent Assembly Properties. J. Biol. Chem. 273: 35176-35184 [Abstract] [Full Text]  
• Soini, Y., Paakko, P., Lehto, V-P. (1998). Histopathological Evaluation of Apoptosis in Cancer. Am. J. Pathol. 153: 1041-1053 [Full Text]  
• Dittadi, R., Coradini, D., Meo, S., Pirronello, E., Daidone, M. G., Gion, M. (1998). Tissue Polypeptide Antigen as a Putative Indicator of Apoptosis. Clin. Chem. 44: 2002-2003 [Full Text]  
• Humke, E. W., Ni, J., Dixit, V. M. (1998). ERICE, a Novel FLICE-activatable Caspase. J. Biol. Chem. 273: 15702-15707 [Abstract] [Full Text]  
• Cryns, V., Yuan, J. (1998). Proteases to die for. Genes Dev. 12: 1551-1570 [Full Text]  
• Zapata, J. M., Takahashi, R., Salvesen, G. S., Reed, J. C. (1998). Granzyme Release and Caspase Activation in Activated Human T-Lymphocytes. J. Biol. Chem. 273: 6916-6920 [Abstract] [Full Text]  
• Ku, N.-O., Liao, J., Omary, M. B. (1997). Apoptosis Generates Stable Fragments of Human Type I Keratins. J. Biol. Chem. 272: 33197-33203 [Abstract] [Full Text]  
• Steinhusen, U., Badock, V., Bauer, A., Behrens, J., Wittman-Liebold, B., Dorken, B., Bommert, K. (2000). Apoptosis-induced Cleavage of beta -Catenin by Caspase-3 Results in Proteolytic Fragments with Reduced Transactivation Potential. J. Biol. Chem. 275: 16345-16353 [Abstract] [Full Text]  
• Steinhusen, U., Weiske, J., Badock, V., Tauber, R., Bommert, K., Huber, O. (2001). Cleavage and Shedding of E-cadherin after Induction of Apoptosis. J. Biol. Chem. 276: 4972-4980 [Abstract] [Full Text]  
• Ku, N.-O., Omary, M. B. (2001). Effect of Mutation and Phosphorylation of Type I Keratins on Their Caspase-mediated Degradation. J. Biol. Chem. 276: 26792-26798 [Abstract] [Full Text]  

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