JCB logo
PeproTech: Free Shipping at www.peprotech.com
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
This Article
Right arrow Full Text
Right arrow Full Text (PDF, 3038K)
Right arrow PPT slides of all figures
Right arrow Alert me when this article is cited
Right arrow Citation Map
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new content in the JCB
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by McNagny, K. M.
Right arrow Articles by Graf, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by McNagny, K. M.
Right arrow Articles by Graf, T.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Facebook   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

© The Rockefeller University Press, 0021-9525/1997//1395 $5.00
The Journal of Cell Biology, Volume 138, Number 6, , 1997 1395-1407

Article

Thrombomucin, a Novel Cell Surface Protein that Defines Thrombocytes and Multipotent Hematopoietic Progenitors



Kelly M. McNagny*, Inger Pettersson*, Fabio Rossi*, Ingo Flamme§, Andrej Shevchenko{ddagger}, Matthias Mann{ddagger}, and Thomas Graf*

* Cell Regulation Program, and {ddagger} Protein and Peptide Program, European Molecular Biology Laboratory, Heidelberg, D-69117 Germany; and § Max-Planck Institut for Physiology and Clinical Research, W.G. Kerckhoff-Institute, Department of Molecular Cell Biology, Bad Nauheim, D-61231 Germany

MEP21 is an avian antigen specifically expressed on the surface of Myb-Ets–transformed multipotent hematopoietic precursors (MEPs) and of normal thrombocytes. Using nanoelectrospray tandem mass spectrometry, we have sequenced and subsequently cloned the MEP21 cDNA and named the gene thrombomucin as it encodes a 571–amino acid protein with an extracellular domain typical of the mucin family of proteoglycans. Thrombomucin is distantly related to CD34, the best characterized and most used human hematopoietic stem cell marker. It is also highly homologous in its transmembrane/intracellular domain to podocalyxinlike protein–1, a rabbit cell surface glycoprotein of kidney podocytes.

Single cell analysis of yolk sac cells from 3-d-old chick embryos revealed that thrombomucin is expressed on the surface of both lineage-restricted and multipotent progenitors. In the bone marrow, thrombomucin is also expressed on mono- and multipotent progenitors, showing an overlapping but distinct expression pattern from that of the receptor-type stem cell marker c-kit. These observations strengthen the notion that the Myb-Ets oncoprotein can induce the proliferation of thrombomucin-positive hematopoietic progenitors that have retained the capacity to differentiate along multiple lineages. They also suggest that thrombomucin and CD34 form a family of stem cell–specific proteins with possibly overlapping functions in early hematopoietic progenitors.

Abbreviations used in this paper: cMGF, chicken myelomonocytic growth factor; MEP, Myb-Ets-transformed progenitors; PCLP, podocalyxinlike protein; SCF, stem cell factor.

Please address all correspondence to Thomas Graf, European Molecular Biology Laboratory, D-69117 Heidelberg, Germany. Tel.: 49-62-21-387-411. Fax: 49-62-21-387-516. e-mail: Graf{at}EMBL-Heidelberg.de

Work in M. Mann's laboratory is partially supported by a grant from the German Technology Ministry (BMFT). K.M. McNagny was supported by NRSA Fellowship No. F32 HL0736 from the National Heart, Lung, and Blood Institute, National Institutes of Health, and a grant from the Deutsche Forschungsgemeinschaft (SFB229) to T. Graf.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Facebook Facebook   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:



  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents