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© The Rockefeller University Press, 0021-9525/1997//1 $5.00
The Journal of Cell Biology, Volume 139, Number 1, , 1997 1-12

Elasticity and Structure of Eukaryote Chromosomes Studied by Micromanipulation and Micropipette Aspiration

Bahram Houchmandzadeh^*,, John F. Marko^,, Didier Chatenay^,||, and Albert Libchaber^,¶

^* Centre National de la Recherche Scientifique (CNRS), Laboratoire de Spectrométrie Physique, 38402 Saint-Martin-d'Hères Cedex, France; Center for Studies in Physics and Biology, The Rockefeller University, New York 10021-6399; Department of Physics, The University of Illinois at Chicago, Chicago, Illinois 60607-7059; ^|| Université Louis Pasteur, CNRS, Institut de Physique, 67000 Strasbourg, France; and ^¶ NEC Research Institute, Princeton, New Jersey 08540

The structure of mitotic chromosomes in cultured newt lung cells was investigated by a quantitative study of their deformability, using micropipettes. Metaphase chromosomes are highly extensible objects that return to their native shape after being stretched up to 10 times their normal length. Larger deformations of 10 to 100 times irreversibly and progressively transform the chromosomes into a "thin filament," parts of which display a helical organization. Chromosomes break for elongations of the order of 100 times, at which time the applied force is around 100 nanonewtons. We have also observed that as mitosis proceeds from nuclear envelope breakdown to metaphase, the native chromosomes progressively become more flexible. (The elastic Young modulus drops from 5,000 ± 1,000 to 1,000 ± 200 Pa.) These observations and measurements are in agreement with a helix-hierarchy model of chromosome structure. Knowing the Young modulus allows us to estimate that the force exerted by the spindle on a newt chromosome at anaphase is roughly one nanonewton.

Address all correspondence to Bahram Houchmandzadeh, CNRS, Laboratorie de Spectrométrie Physique, BP 57, 38402 Saint-Martin-d'Hères Cedex, France. Tel.: (33) 476 51 44 27. Fax: (33) 476 51 45 44. E-mail: bahram{at}coucou.ujf-grenoble.fr

We warmly thank M. Elbaum who developed the micromanipulation set-up, and S. Childress, M. Goulian, T. Hirano, H. Macgregor, W. Marshall, P. Moens, Y. Rabin, E. Siggia, and J. Swedlow for many helpful discussions.

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