The Microtubule-dependent Motor Centromere-associated Protein E (CENP-E) Is an Integral Component of Kinetochore Corona Fibers That Link Centromeres to Spindle Microtubules

doi:10.1083/jcb.139.2.435

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© The Rockefeller University Press, 0021-9525/1997//435 $5.00
The Journal of Cell Biology, Volume 139, Number 2, , 1997 435-447

Article

The Microtubule-dependent Motor Centromere–associated Protein E (CENP-E) Is an Integral Component of Kinetochore Corona Fibers That Link Centromeres to Spindle Microtubules

Xuebiao Yao^*, Karen L. Anderson^*, and Don W. Cleveland^*^,

^* Laboratory of Cell Biology, Ludwig Institute for Cancer Research, Division of Cellular and Molecular Medicine, School of Medicine, University of California, San Diego, La Jolla, California 92093-0660

Centromere-associated protein E (CENP-E) is a kinesin-relatedmicrotubule motor protein that is essential for chromosomecongression during mitosis. Using immunoelectron microscopy,CENP-E is shown to be an integral component of the kinetochorecorona fibers that tether centromeres to the spindle. Immediatelyupon nuclear envelope fragmentation, an associated plus endmotor trafficks cytoplasmic CENP-E toward chromosomes alongastral microtubules that enter the nuclear volume. Before orconcurrently with initial lateral attachment of spindle microtubules, CENP-E targets to the outermost region of the developing kinetochores.After stable attachment, throughout chromosome congression,at metaphase, and throughout anaphase A, CENP-E is a constituentof the corona fibers, extending at least 50 nm away from thekinetochore outer plate and intertwining with spindle microtubules.In congressing chromosomes, CENP-E is preferentially associatedwith (or accessible at) the stretched, leading kinetochoreknown to provide the primary power for chromosome movement.Taken together, this evidence strongly supports a model in which CENP-E functions in congression to tether kinetochoresto the disassembling microtubule plus ends.

Address all correspondence to Dr. Don W. Cleveland, 3080 CMM-East, University of California, 9500 Gilman Drive, La Jolla, CA 92093-0660. Tel.: (619) 534-7811. Fax: (619) 534-7659.

1. Abbreviation used in this paper: CENP, centromere-associatedprotein.

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