J. Cell Biol., Volume 139, Number 3, November 3, 1997 639-649

Major Histocompatibility Complex Class II Compartments in Human and Mouse B Lymphoblasts Represent Conventional Endocytic Compartments

Monique J. Kleijmeer,^* Stanislaw Morkowski, Janice M. Griffith,^* Alexander Y. Rudensky,^§ and Hans J. Geuze^*

^* Department of Cell Biology, School of Medicine and Institute of Biomembranes, Utrecht University, 3584 CX Utrecht, The Netherlands; and  Department of Immunology and ^§ Howard Hughes Medical Institute, University of Washington, School of Medicine, Seattle, Washington 98105

In most human and mouse antigen-presenting cells, the majority of intracellular major histocompatibility complex (MHC) class II molecules resides in late endocytic MHC class II compartments (MIICs), thought to function in antigen processing and peptide loading. However, in mouse A20 B cells, early endocytic class II-containing vesicles (CIIVs) have been reported to contain most of the intracellular MHC class II molecules and have also been implicated in formation of MHC class II-peptide complexes. To address this discrepancy, we have studied in great detail the endocytic pathways of both a human (6H5.DM) and a mouse (A20.A^b) B cell line. Using quantitative immunoelectron microscopy on cryosections of cells that had been pulse-chased with transferrin-HRP or BSA-gold as endocytic tracers, we have identified up to six endocytic subcompartments including an early MIIC type enriched in invariant chain, suggesting that it serves as an important entrance to the endocytic pathway for newly synthesized MHC class II/invariant chain complexes. In addition, early MIICs represented the earliest endocytic compartment containing MHC class II- peptide complexes, as shown by using an antibody against an abundant endogenous class II-peptide complex. The early MIIC exhibited several though not all of the characteristics reported for the CIIV and was situated just downstream of early endosomes. We have not encountered any special class II-containing endocytic structures besides those normally present in nonantigen-presenting cells. Our results therefore suggest that B cells use conventional endocytic compartments rather than having developed a unique compartment to accomplish MHC class II presentation.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

• Sadaka, C., Marloie-Provost, M.-A., Soumelis, V., Benaroch, P. (2009). Developmental regulation of MHC II expression and transport in human plasmacytoid-derived dendritic cells. Blood 113: 2127-2135 [Abstract] [Full Text]  
• Berger, A. C., Roche, P. A. (2009). MHC class II transport at a glance. J. Cell Sci. 122: 1-4 [Full Text]  
• Hundorfean, G., Zimmer, K.-P., Strobel, S., Gebert, A., Ludwig, D., Buning, J. (2007). Luminal antigens access late endosomes of intestinal epithelial cells enriched in MHC I and MHC II molecules: in vivo study in Crohn's ileitis. Am. J. Physiol. Gastrointest. Liver Physiol. 293: G798-G808 [Abstract] [Full Text]  
• Mellman, I. (2007). Private Lives: Reflections and Challenges in Understanding the Cell Biology of the Immune System. Science 317: 625-627 [Abstract] [Full Text]  
• Polson, A. G., Yu, S.-F., Elkins, K., Zheng, B., Clark, S., Ingle, G. S., Slaga, D. S., Giere, L., Du, C., Tan, C., Hongo, J.-A., Gogineni, A., Cole, M. J., Vandlen, R., Stephan, J.-P., Young, J., Chang, W., Scales, S. J., Ross, S., Eaton, D., Ebens, A. (2007). Antibody-drug conjugates targeted to CD79 for the treatment of non-Hodgkin lymphoma. Blood 110: 616-623 [Abstract] [Full Text]  
• Majewski, M., Bose, T. O., Sille, F. C. M., Pollington, A. M., Fiebiger, E., Boes, M. (2007). Protein kinase C delta stimulates antigen presentation by Class II MHC in murine dendritic cells. Int Immunol 19: 719-732 [Abstract] [Full Text]  
• Kamsteeg, E.-J., Hendriks, G., Boone, M., Konings, I. B. M., Oorschot, V., van der Sluijs, P., Klumperman, J., Deen, P. M. T. (2006). Short-chain ubiquitination mediates the regulated endocytosis of the aquaporin-2 water channel. Proc. Natl. Acad. Sci. USA 103: 18344-18349 [Abstract] [Full Text]  
• Finzi, A., Brunet, A., Xiao, Y., Thibodeau, J., Cohen, E. A. (2006). Major Histocompatibility Complex Class II Molecules Promote Human Immunodeficiency Virus Type 1 Assembly and Budding to Late Endosomal/Multivesicular Body Compartments. J. Virol. 80: 9789-9797 [Abstract] [Full Text]  
• Potolicchio, I., Chitta, S., Xu, X., Fonseca, D., Crisi, G., Horejsi, V., Strominger, J. L., Stern, L. J., Raposo, G., Santambrogio, L. (2005). Conformational Variation of Surface Class II MHC Proteins during Myeloid Dendritic Cell Differentiation Accompanies Structural Changes in Lysosomal MIIC. J. Immunol. 175: 4935-4947 [Abstract] [Full Text]  
• Singleton, T. E., Massari, P., Wetzler, L. M. (2005). Neisserial Porin-Induced Dendritic Cell Activation Is MyD88 and TLR2 Dependent. J. Immunol. 174: 3545-3550 [Abstract] [Full Text]  
• Smyth, C. M., Logan, G., Boadle, R., Rowe, P. B., Smythe, J. A., Alexander, I. E. (2005). Differential subcellular localization of CD86 in human PBMC-derived macrophages and DCs, and ultrastructural characterization by immuno-electron microscopy. Int Immunol 17: 123-132 [Abstract] [Full Text]  
• Coffey, G. P., Stefanich, E., Palmieri, S., Eckert, R., Padilla-Eagar, J., Fielder, P. J., Pippig, S. (2004). In Vitro Internalization, Intracellular Transport, and Clearance of an Anti-CD11a Antibody (Raptiva) by Human T-Cells. J. Pharmacol. Exp. Ther. 310: 896-904 [Abstract] [Full Text]  
• Muntasell, A., Carrascal, M., Alvarez, I., Serradell, L., van Veelen, P., Verreck, F. A. W., Koning, F., Abian, J., Jaraquemada, D. (2004). Dissection of the HLA-DR4 Peptide Repertoire in Endocrine Epithelial Cells: Strong Influence of Invariant Chain and HLA-DM Expression on the Nature of Ligands. J. Immunol. 173: 1085-1093 [Abstract] [Full Text]  
• Lautwein, A., Kraus, M., Reich, M., Burster, T., Brandenburg, J., Overkleeft, H. S., Schwarz, G., Kammer, W., Weber, E., Kalbacher, H., Nordheim, A., Driessen, C. (2004). Human B lymphoblastoid cells contain distinct patterns of cathepsin activity in endocytic compartments and regulate MHC class II transport in a cathepsin S-independent manner. J. Leukoc. Biol. 75: 844-855 [Abstract] [Full Text]  
• Lambert, O., Cavusoglu, N., Gallay, J., Vincent, M., Rigaud, J. L., Henry, J.-P., Ayala-Sanmartin, J. (2004). Novel Organization and Properties of Annexin 2-Membrane Complexes. J. Biol. Chem. 279: 10872-10882 [Abstract] [Full Text]  
• Lin, S. X., Mallet, W. G., Huang, A. Y., Maxfield, F. R. (2004). Endocytosed Cation-Independent Mannose 6-Phosphate Receptor Traffics via the Endocytic Recycling Compartment en Route to the trans-Golgi Network and a Subpopulation of Late Endosomes. Mol. Biol. Cell 15: 721-733 [Abstract] [Full Text]  
• Murk, J. L. A. N., Humbel, B. M., Ziese, U., Griffith, J. M., Posthuma, G., Slot, J. W., Koster, A. J., Verkleij, A. J., Geuze, H. J., Kleijmeer, M. J. (2003). Endosomal compartmentalization in three dimensions: Implications for membrane fusion. Proc. Natl. Acad. Sci. USA 100: 13332-13337 [Abstract] [Full Text]  
• Boes, M., Bertho, N., Cerny, J., Op den Brouw, M., Kirchhausen, T., Ploegh, H. (2003). T Cells Induce Extended Class II MHC Compartments in Dendritic Cells in a Toll-Like Receptor-Dependent Manner. J. Immunol. 171: 4081-4088 [Abstract] [Full Text]  
• Marques, E. T. A. Jr., Chikhlikar, P., de Arruda, L. B., Leao, I. C., Lu, Y., Wong, J., Chen, J.-S., Byrne, B., August, J. T. (2003). HIV-1 p55Gag Encoded in the Lysosome-associated Membrane Protein-1 as a DNA Plasmid Vaccine Chimera Is Highly Expressed, Traffics to the Major Histocompatibility Class II Compartment, and Elicits Enhanced Immune Responses. J. Biol. Chem. 278: 37926-37936 [Abstract] [Full Text]  
• Chalouni, C., Banchereau, J., Vogt, A. B., Pascual, V., Davoust, J. (2003). Human germinal center B cells differ from naive and memory B cells by their aggregated MHC class II-rich compartments lacking HLA-DO. Int Immunol 15: 457-466 [Abstract] [Full Text]  
• Wubbolts, R., Leckie, R. S., Veenhuizen, P. T. M., Schwarzmann, G., Mobius, W., Hoernschemeyer, J., Slot, J.-W., Geuze, H. J., Stoorvogel, W. (2003). Proteomic and Biochemical Analyses of Human B Cell-derived Exosomes. POTENTIAL IMPLICATIONS FOR THEIR FUNCTION AND MULTIVESICULAR BODY FORMATION. J. Biol. Chem. 278: 10963-10972 [Abstract] [Full Text]  
• Greiner, A., Lautwein, A., Overkleeft, H. S., Weber, E., Driessen, C. (2003). Activity and subcellular distribution of cathepsins in primary human monocytes. J. Leukoc. Biol. 73: 235-242 [Abstract] [Full Text]  
• Granboulan, M., Lankar, D., Raposo, G., Bonnerot, C., Hivroz, C. (2003). Phosphoinositide 3-Kinase Activation by Igbeta Controls de Novo Formation of an Antigen-processing Compartment. J. Biol. Chem. 278: 4331-4338 [Abstract] [Full Text]  
• Muntasell, A., Carrascal, M., Serradell, L., Veelen, P. v., Verreck, F., Koning, F., Raposo, G., Abian, J., Jaraquemada, D. (2002). HLA-DR4 Molecules in Neuroendocrine Epithelial Cells Associate to a Heterogeneous Repertoire of Cytoplasmic and Surface Self Peptides. J. Immunol. 169: 5052-5060 [Abstract] [Full Text]  
• Li, C., Siemasko, K., Clark, M. R., Song, W. (2002). Cooperative interaction of Ig{alpha} and Ig{beta} of the BCR regulates the kinetics and specificity of antigen targeting. Int Immunol 14: 1179-1191 [Abstract] [Full Text]  
• Zaliauskiene, L., Kang, S., Sparks, K., Zinn, K. R., Schwiebert, L. M., Weaver, C. T., Collawn, J. F. (2002). Enhancement of MHC Class II-Restricted Responses by Receptor-Mediated Uptake of Peptide Antigens. J. Immunol. 169: 2337-2345 [Abstract] [Full Text]  
• Siemasko, K., Skaggs, B. J., Kabak, S., Williamson, E., Brown, B. K., Song, W., Clark, M. R. (2002). Receptor-Facilitated Antigen Presentation Requires the Recruitment of B Cell Linker Protein to Ig{alpha}. J. Immunol. 168: 2127-2138 [Abstract] [Full Text]  
• Kolesnikova, L., Bugany, H., Klenk, H.-D., Becker, S. (2002). VP40, the Matrix Protein of Marburg Virus, Is Associated with Membranes of the Late Endosomal Compartment. J. Virol. 76: 1825-1838 [Abstract] [Full Text]  
• Mobius, W., Ohno-Iwashita, Y., van Donselaar, E. G., Oorschot, V. M.J., Shimada, Y., Fujimoto, T., Heijnen, H. F.G., Geuze, H. J., Slot, J. W. (2002). Immunoelectron Microscopic Localization of Cholesterol Using Biotinylated and Non-cytolytic Perfringolysin O. J. Histochem. Cytochem. 50: 43-56 [Abstract] [Full Text]  
• Valladeau, J., Duvert-Frances, V., Pin, J.-J., Kleijmeer, M. J., Ait-Yahia, S., Ravel, O., Vincent, C., Vega, F. Jr., Helms, A., Gorman, D., Zurawski, S. M., Zurawski, G., Ford, J., Saeland, S. (2001). Immature Human Dendritic Cells Express Asialoglycoprotein Receptor Isoforms for Efficient Receptor-Mediated Endocytosis. J. Immunol. 167: 5767-5774 [Abstract] [Full Text]  
• Ramachandra, L., Noss, E., Boom, W. H., Harding, C. V. (2001). Processing of Mycobacterium tuberculosis Antigen 85B Involves Intraphagosomal Formation of Peptide-Major Histocompatibility Complex II Complexes and Is Inhibited by Live Bacilli that Decrease Phagosome Maturation. JEM 194: 1421-1432 [Abstract] [Full Text]  
• Kleijmeer, M., Ramm, G., Schuurhuis, D., Griffith, J., Rescigno, M., Ricciardi-Castagnoli, P., Rudensky, A. Y., Ossendorp, F., Melief, C. J.M., Stoorvogel, W., Geuze, H. J. (2001). Reorganization of multivesicular bodies regulates MHC class II antigen presentation by dendritic cells. JCB 155: 53-64 [Abstract] [Full Text]  
• van Kerkhof, P., dos Santos, C. M. A., Sachse, M., Klumperman, J., Bu, G., Strous, G. J. (2001). Proteasome Inhibitors Block a Late Step in Lysosomal Transport of Selected Membrane but not Soluble Proteins. Mol. Biol. Cell 12: 2556-2566 [Abstract] [Full Text]  
• Kujala, P., Ikäheimonen, A., Ehsani, N., Vihinen, H., Auvinen, P., Kääriäinen, L. (2001). Biogenesis of the Semliki Forest Virus RNA Replication Complex. J. Virol. 75: 3873-3884 [Abstract] [Full Text]  
• Baron, C, Raposo, G, Scholl, S., Bausinger, H, Tenza, D, Bohbot, A, Pouillart, P, Goud, B, Hanau, D, Salamero, J (2001). Modulation of MHC class II transport and lysosome distribution by macrophage-colony stimulating factor in human dendritic cells derived from monocytes. J. Cell Sci. 114: 999-1010 [Abstract]  
• Forestier, C., Deleuil, F., Lapaque, N., Moreno, E., Gorvel, J.-P. (2000). Brucella abortus Lipopolysaccharide in Murine Peritoneal Macrophages Acts as a Down-Regulator of T Cell Activation. J. Immunol. 165: 5202-5210 [Abstract] [Full Text]  
• Denzer, K., van Eijk, M., Kleijmeer, M. J., Jakobson, E., de Groot, C., J. Geuze, H. (2000). Follicular Dendritic Cells Carry MHC Class II-Expressing Microvesicles at Their Surface. J. Immunol. 165: 1259-1265 [Abstract] [Full Text]  
• Chames, P., Hufton, S. E., Coulie, P. G., Uchanska-Ziegler, B., Hoogenboom, H. R. (2000). Direct selection of a human antibody fragment directed against the tumor T-cell epitope HLA-A1-MAGE-A1 from a nonimmunized phage-Fab library. Proc. Natl. Acad. Sci. USA 97: 7969-7974 [Abstract] [Full Text]  
• DELL’ANGELICA, E. C., MULLINS, C., CAPLAN, S., BONIFACINO, J. S. (2000). Lysosome-related organelles. FASEB J. 14: 1265-1278 [Abstract] [Full Text]  
• Ramachandra, L., Harding, C. V. (2000). Phagosomes Acquire Nascent and Recycling Class II MHC Molecules but Primarily Use Nascent Molecules in Phagocytic Antigen Processing. J. Immunol. 164: 5103-5112 [Abstract] [Full Text]  
• Hausmann, G., O'Reilly, L. A., van Driel, R., Beaumont, J. G., Strasser, A., Adams, J. M., Huang, D. C.S. (2000). Pro-apoptotic Apoptosis Protease-activating Factor 1 (Apaf-1) Has a Cytoplasmic Localization Distinct from Bcl-2 or Bcl-xL. JCB 149: 623-634 [Abstract] [Full Text]  
• Turley, S. J., Inaba, K., Garrett, W. S., Ebersold, M., Unternaehrer, J., Steinman, R. M., Mellman, I. (2000). Transport of Peptide-MHC Class II Complexes in Developing Dendritic Cells. Science 288: 522-527 [Abstract] [Full Text]  
• Hess, M. W., Schwendinger, M. G., Eskelinen, E.-L., Pfaller, K., Pavelka, M., Dierich, M. P., Prodinger, W. M. (2000). Tracing uptake of C3dg-conjugated antigen into B cells via complement receptor type 2 (CR2, CD21). Blood 95: 2617-2623 [Abstract] [Full Text]  
• Arunachalam, B., Phan, U. T., Geuze, H. J., Cresswell, P. (2000). Enzymatic reduction of disulfide bonds in lysosomes: Characterization of a Gamma-interferon-inducible lysosomal thiol reductase (GILT). Proc. Natl. Acad. Sci. USA 97: 745-750 [Abstract] [Full Text]  
• Reaves, B., Row, P., Bright, N., Luzio, J., Davidson, H. (2000). Loss of cation-independent mannose 6-phosphate receptor expression promotes the accumulation of lysobisphosphatidic acid in multilamellar bodies. J. Cell Sci. 113: 4099-4108 [Abstract]  
• Denzer, K, Kleijmeer, M., Heijnen, H., Stoorvogel, W, Geuze, H. (2000). Exosome: from internal vesicle of the multivesicular body to intercellular signaling device. J. Cell Sci. 113: 3365-3374 [Abstract]  
• Ramm, G, Pond, L, Watts, C, Stoorvogel, W (2000). Clathrin-coated lattices and buds on MHC class II compartments do not selectively recruit mature MHC-II. J. Cell Sci. 113: 303-313 [Abstract]  
• de Wit, H., Lichtenstein, Y., Geuze, H. J., Kelly, R. B., van der Sluijs, P., Klumperman, J. (1999). Synaptic Vesicles Form by Budding from Tubular Extensions of Sorting Endosomes in PC12 Cells. Mol. Biol. Cell 10: 4163-4176 [Abstract] [Full Text]  
• Driessen, C., Bryant, R. A.R., Lennon-Dumenil, A.-M., Villadangos, J. A., Bryant, P. W., Shi, G.-P., Chapman, H. A., Ploegh, H. L. (1999). Cathepsin S Controls the Trafficking and Maturation of MHC Class II Molecules in Dendritic Cells. JCB 147: 775-790 [Abstract] [Full Text]  
• Davidson, H. W. (1999). Direct Transport of Newly Synthesized HLA-DR from the trans-Golgi Network to Major Histocompatibility Complex Class II Containing Compartments (MIICS) Demonstrated Using a Novel Tyrosine-sulfated Chimera. J. Biol. Chem. 274: 27315-27322 [Abstract] [Full Text]  
• van der Meer, Y., Snijder, E. J., Dobbe, J. C., Schleich, S., Denison, M. R., Spaan, W. J. M., Locker, J. K. (1999). Localization of Mouse Hepatitis Virus Nonstructural Proteins and RNA Synthesis Indicates a Role for Late Endosomes in Viral Replication. J. Virol. 73: 7641-7657 [Abstract] [Full Text]  
• Simonsen, A., Pedersen, K. W., Nordeng, T. W., von der Lippe, A., Stang, E., Long, E. O., Bakke, O. (1999). Polarized Transport of MHC Class II Molecules in Madin-Darby Canine Kidney Cells Is Directed by a Leucine-Based Signal in the Cytoplasmic Tail of the {beta}-Chain. J. Immunol. 163: 2540-2548 [Abstract] [Full Text]  
• Brachet, V., Péhau-Arnaudet, G., Desaymard, C., Raposo, G., Amigorena, S. (1999). Early Endosomes Are Required for Major Histocompatiblity Complex Class II Transport to Peptide-loading Compartments. Mol. Biol. Cell 10: 2891-2904 [Abstract] [Full Text]  
• Advani, R. J., Yang, B., Prekeris, R., Lee, K. C., Klumperman, J., Scheller, R. H. (1999). VAMP-7 Mediates Vesicular Transport from Endosomes to Lysosomes. JCB 146: 765-776 [Abstract] [Full Text]  
• Barrat, F. J., Le Deist, F., Benkerrou, M., Bousso, P., Feldmann, J., Fischer, A., de Saint Basile, G. (1999). Defective CTLA-4 cycling pathway in Chediak-Higashi syndrome: A possible mechanism for deregulation of T lymphocyte activation. Proc. Natl. Acad. Sci. USA 96: 8645-8650 [Abstract] [Full Text]  
• van Vugt, M. J., Kleijmeer, M. J., Keler, T., Zeelenberg, I., van Dijk, M. A., Leusen, J. H.W., Geuze, H. J., van de Winkel, J. G.J. (1999). The Fcgamma RIa (CD64) Ligand Binding Chain Triggers Major Histocompatibility Complex Class II Antigen Presentation Independently of Its Associated FcR gamma -Chain. Blood 94: 808-817 [Abstract] [Full Text]  
• Pond, L., Watts, C. (1999). Functional Early Endosomes Are Required for Maturation of Major Histocompatibility Complex Class II Molecules in Human B Lymphoblastoid Cells. J. Biol. Chem. 274: 18049-18054 [Abstract] [Full Text]  
• Beatty, W. L., Meresse, S., Gounon, P., Davoust, J., Mounier, J., Sansonetti, P. J., Gorvel, J.-P. (1999). Trafficking of Shigella Lipopolysaccharide in Polarized Intestinal Epithelial Cells. JCB 145: 689-698 [Abstract] [Full Text]  
• Brown, B. K., Li, C., Cheng, P. C., Song, W. (1999). Trafficking of the Igalpha /Igbeta Heterodimer with Membrane Ig and Bound Antigen to the Major Histocompatibility Complex Class II Peptide-loading Compartment. J. Biol. Chem. 274: 11439-11446 [Abstract] [Full Text]  
• Ramachandra, L., Song, R., Harding, C. V. (1999). Phagosomes Are Fully Competent Antigen-Processing Organelles That Mediate the Formation of Peptide:Class II MHC Complexes. J. Immunol. 162: 3263-3272 [Abstract] [Full Text]  
• Drake, J. R., Lewis, T. A., Condon, K. B., Mitchell, R. N., Webster, P. (1999). Involvement of MIIC-Like Late Endosomes in B Cell Receptor-Mediated Antigen Processing in Murine B Cells. J. Immunol. 162: 1150-1155 [Abstract] [Full Text]  
• Antoine, J., Lang, T, Prina, E, Courret, N, Hellio, R (1999). H-2M molecules, like MHC class II molecules, are targeted to parasitophorous vacuoles of Leishmania-infected macrophages and internalized by amastigotes of L. amazonensis and L. mexicana. J. Cell Sci. 112: 2559-2570 [Abstract]  
• Mallet, W. G., Maxfield, F. R. (1999). Chimeric Forms of Furin and TGN38 Are Transported from the Plasma Membrane to the Trans-Golgi Network via Distinct Endosomal Pathways. JCB 146: 345-360 [Abstract] [Full Text]  
• Lem, L., Riethof, D. A., Scidmore-Carlson, M., Griffiths, G. M., Hackstadt, T., Brodsky, F. M. (1999). Enhanced Interaction of HLA-DM with HLA-DR in Enlarged Vacuoles of Hereditary and Infectious Lysosomal Diseases. J. Immunol. 162: 523-532 [Abstract] [Full Text]  
• Hammond, C., Denzin, L. K., Pan, M., Griffith, J. M., Geuze, H. J., Cresswell, P. (1998). The Tetraspan Protein CD82 Is a Resident of MHC Class II Compartments Where It Associates with HLA-DR, -DM, and -DO Molecules. J. Immunol. 161: 3282-3291 [Abstract] [Full Text]  
• Maurer, D., Fiebiger, E., Reininger, B., Ebner, C., Petzelbauer, P., Shi, G.-P., Chapman, H. A., Stingl, G. (1998). Fc{epsilon} Receptor I on Dendritic Cells Delivers IgE-Bound Multivalent Antigens into a Cathepsin S-Dependent Pathway of MHC Class II Presentation. J. Immunol. 161: 2731-2739 [Abstract] [Full Text]  
• Thery, C., Brachet, V., Regnault, A., Rescigno, M., Ricciardi-Castagnoli, P., Bonnerot, C., Amigorena, S. (1998). MHC Class II Transport from Lysosomal Compartments to the Cell Surface Is Determined by Stable Peptide Binding, But Not by the Cytosolic Domains of the {alpha}- and {beta}-Chains. J. Immunol. 161: 2106-2113 [Abstract] [Full Text]  
• Escola, J.-M., Kleijmeer, M. J., Stoorvogel, W., Griffith, J. M., Yoshie, O., Geuze, H. J. (1998). Selective Enrichment of Tetraspan Proteins on the Internal Vesicles of Multivesicular Endosomes and on Exosomes Secreted by Human B-lymphocytes. J. Biol. Chem. 273: 20121-20127 [Abstract] [Full Text]  
• Faigle, W., Raposo, G., Tenza, D., Pinet, V., Vogt, A. B., Kropshofer, H., Fischer, A., de Saint-Basile, G., Amigorena, S. (1998). Deficient Peptide Loading and MHC Class II Endosomal Sorting in a Human Genetic Immunodeficiency Disease: the Chediak-Higashi Syndrome. JCB 141: 1121-1134 [Abstract] [Full Text]  
• Guerra, C. B., Busch, R., Doebele, R. C., Liu, W., Sawada, T., Kwok, W. W., Chang, M.-d. Y., Mellins, E. D. (1998). Novel Glycosylation of HLA-DR{alpha} Disrupts Antigen Presentation Without Altering Endosomal Localization. J. Immunol. 160: 4289-4297 [Abstract] [Full Text]  
• Harsay, E., Schekman, R. (2002). A subset of yeast vacuolar protein sorting mutants is blocked in one branch of the exocytic pathway. JCB 156: 271-286 [Abstract] [Full Text]  
• Sachse, M., Urbe, S., Oorschot, V., Strous, G. J., Klumperman, J. (2002). Bilayered Clathrin Coats on Endosomal Vacuoles Are Involved in Protein Sorting toward Lysosomes. Mol. Biol. Cell 13: 1313-1328 [Abstract] [Full Text]  

Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents