A Yeast Mutant Showing Diagnostic Markers of Early and Late Apoptosis

doi:10.1083/jcb.139.3.729

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© The Rockefeller University Press, 0021-9525/1997//729 $5.00
The Journal of Cell Biology, Volume 139, Number 3, , 1997 729-734

Article

A Yeast Mutant Showing Diagnostic Markers of Early and Late Apoptosis

Frank Madeo^*, Eleonore Fröhlich, and Kai-Uwe Fröhlich^*

^* Physiologisch-chemisches Institut, Universität Tübingen, 72076 Tübingen, Germany; and Anatomisches Institut, Universität Tübingen, 72072 Tübingen, Germany

A Saccharomyces cerevisiae mutant in cell division cycle geneCDC48 shows typical markers of apoptosis: membrane stainingwith annexin V, indicating an exposure of phosphatidylserineat the outer layer of the cytoplasmic membrane; intense staining,using the terminal deoxynucleotidyl transferase–mediateddUTP nick end labeling method, indicating DNA fragmentation;and chromatin condensation and fragmentation. The coordinateoccurrence of these events at different locations in the cell,which have no obvious connection except their relation to apoptosis,implies the presence of the molecular machinery performingthe basic steps of apoptosis already in yeast. Saccharomycescerevisiae may prove a suitable model to trace the roots ofapoptosis.

Abbreviations used in this paper: ICE, IL-1β–converting enzyme; TUNEL, terminal deoxynucleotidyl transferase–mediated dUTP nick end labeling.

Address all correspondence to Kai-Uwe Fröhlich, Physiologisch-ChemischesInstitut, Hoppe-Seyler-Strasse 4, Universität Tübingen,72076 Tübingen, Germany. Tel.: 49-7071-297-3360. Fax:49-7071-296-6390. E-mail: kaifr{at}uni-tuebingen.de

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