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© The Rockefeller University Press, 0021-9525/1997//951 $5.00
The Journal of Cell Biology, Volume 139, Number 4, , 1997 951-961

Article

Neurabin: A Novel Neural Tissue–specific Actin Filament–binding Protein Involved in Neurite Formation



Hiroyuki Nakanishi*, Hiroshi Obaishi*, Ayako Satoh*, Manabu Wada*, Kenji Mandai*, Keiko Satoh*, Hideo Nishioka*, Yoshiharu Matsuura§, Akira Mizoguchi||, and Yoshimi Takai*,{ddagger}

* Takai Biotimer Project, ERATO, Japan Science and Technology Corporation, c/o JCR Pharmaceuticals Co., Ltd., Nishi-ku, Kobe 651-22, Japan; {ddagger} Department of Molecular Biology and Biochemistry, Osaka University Medical School, Suita 565, Japan; § Department of Virology II, National Institutes of Health, Tokyo 162, Japan; || Department of Anatomy and Neurobiology, Graduate School, Kyoto University, Kyoto 606-01, Japan

We purified from rat brain a novel actin filament (F-actin)–binding protein of ~180 kD (p180), which was specifically expressed in neural tissue. We named p180 neurabin (neural tissue–specific F-actin– binding protein). We moreover cloned the cDNA of neurabin from a rat brain cDNA library and characterized native and recombinant proteins. Neurabin was a protein of 1,095 amino acids with a calculated molecular mass of 122,729. Neurabin had one F-actin–binding domain at the NH2-terminal region, one PSD-95, DlgA, ZO-1–like domain at the middle region, a domain known to interact with transmembrane proteins, and domains predicted to form coiled-coil structures at the COOH-terminal region. Neurabin bound along the sides of F-actin and showed F-actin–cross-linking activity. Immunofluorescence microscopic analysis revealed that neurabin was highly concentrated in the synapse of the developed neurons. Neurabin was also concentrated in the lamellipodia of the growth cone during the development of neurons. Moreover, a study on suppression of endogenous neurabin in primary cultured rat hippocampal neurons by treatment with an antisense oligonucleotide showed that neurabin was involved in the neurite formation. Neurabin is a candidate for key molecules in the synapse formation and function.

Abbreviations used in this paper: aa, amino acid; F-actin, actin filament; G-actin, actin monomer; GST, glutathione-S-transferase; neurabin, neural tissue–specific F-actin–binding protein; PDZ, PSD-95, DlgA, ZO-1-like; PON, phosphorothioate oligonucleotide; S1, subfragment 1; SV, synaptic vesicle.

Address all correspondence to Y. Takai, Department of Molecular Biology and Biochemistry, Osaka University Medical School, Suita 565, Japan. Tel.: 81-6-879-3410. Fax: 81-6-879-3419. E-mail: ytakai{at}molbio.med.osaka-u.ac.jp

Received for publication 7 April 1997 and in revised form 8 August 1997.


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