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© The Rockefeller University Press, 0021-9525/1997//1361 $5.00
The Journal of Cell Biology, Volume 139, Number 6, , 1997 1361-1371

Article

A Chromatin-associated Kinesin-related Protein Required for Normal Mitotic Chromosome Segregation in Drosophila



Isabel Molina*, Sigrid Baars*, Julie A. Brill{ddagger}, Karen G. Hales§, Margaret T. Fuller{ddagger},§, and Pedro Ripoll*

* Centro de Biología Molecular "Severo Ochoa" Consejo Superior Investigaciones Científicas–Universidad Autónoma Madrid, Facultad de Ciencias, UAM, Cantoblanco, 28049 Madrid, Spain; and {ddagger} Department of Developmental Biology, § Department of Genetics, Stanford University School of Medicine, Stanford, California 94305-5427

The tiovivo (tio) gene of Drosophila encodes a kinesin-related protein, KLP38B, that colocalizes with condensed chromatin during cell division. Wild-type function of the tio gene product KLP38B is required for normal chromosome segregation during mitosis. Mitotic cells in tio larval brains displayed circular mitotic figures, increased ploidy, and abnormal anaphase figures. KLP38B mRNA is maternally provided and expressed in cells about to undergo division. We propose that KLP38B, perhaps redundantly with other chromosome-associated microtubule motor proteins, contributes to interactions between chromosome arms and microtubules important for establishing bipolar attachment of chromosomes and assembly of stable bipolar spindles.

Abbreviations used in this paper: CMF, circular mitotic figure; tio, tiovivo.

I. Molina was supported by a Dirección General de Investigación Científica y Técnica (DGICYT) postdoctoral fellowship and a DGICYT reincorporation contract, S. Baars was supported by EU Network No. CECHRX-CT93-0186, J.A. Brill was supported by National Institutes of Health (NIH) postdoctoral Fellowship No. 5-F32-HD07728 and a Katharine D. McCormick Fellowship award, and K.G. Hales was supported by a Howard Hughes Medical Institute predoctoral fellowship. This work was supported by NIH grant No. 5R01-HD29194 to M.T. Fuller and by DGICYT grants PB90-0110 and PB93-0174 and an institutional grant from Fundacion Ramon Areces to P. Ripoli.

Address all correspondence to Margaret Fuller, Department of Developmental Biology, Beckman Center B300, Stanford University School of Medicine, Stanford, CA 94305-5427. Tel.: (650) 725-7681. Fax: (650) 725-7739. E-mail: fuller{at}cmgm.stanford.edu


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