A Distinct and Parallel Pathway for the Nuclear Import of an mRNA-binding Protein

doi:10.1083/jcb.139.7.1645

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© The Rockefeller University Press, 0021-9525/1997//1645 $5.00
The Journal of Cell Biology, Volume 139, Number 7, , 1997 1645-1653

Article

A Distinct and Parallel Pathway for the Nuclear Import of an mRNA-binding Protein

Lucy F. Pemberton, Jonathan S. Rosenblum, and Günter Blobel

Laboratory of Cell Biology, Howard Hughes Medical Institute, The Rockefeller University, New York 10021

Three independent pathways of nuclear import have so far beenidentified in yeast, each mediated by cognate nuclear transportfactors, or karyopherins. Here we have characterized a newpathway to the nucleus, mediated by Mtr10p, a protein firstidentified in a screen for strains defective in polyadenylatedRNA export. Mtr10p is shown to be responsible for the nuclear import of the shuttling mRNA-binding protein Npl3p. A complexof Mtr10p and Npl3p was detected in cytosol, and deletion ofMtr10p was shown to lead to the mislocalization of nuclearNpl3p to the cytoplasm, correlating with a block in import.Mtr10p bound peptide repeat-containing nucleoporins and Ran,suggesting that this import pathway involves a docking stepat the nuclear pore complex and is Ran dependent. This pathwayof Npl3p import is distinct and does not appear to overlapwith another known import pathway for an mRNA-binding protein.Thus, at least two parallel pathways function in the importof mRNA-binding proteins, suggesting the need for the coordinationof these pathways.

Abbreviations used in this paper: ADH, alcohol dehydrogenase; GFP, green fluorescent protein; GST, glutathione-S-transferase; hn, heterogeneous nuclear; NPC, nuclear pore complex.

J.S. Rosenblum was supported by a postdoctoral fellowship fromthe National Institutes of Health.

Address all correspondence to Günter Blobel, Laboratoryof Cell Biology, Howard Hughes Medical Institute, The RockefellerUniversity, New York, NY 10021. Tel.: (212) 327-8181. Fax:(212) 327-7880. E-mail: blobel @rockvax.rockefeller.edu

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