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© The Rockefeller University Press, 0021-9525/1998//101 $5.00
The Journal of Cell Biology, Volume 140, Number 1, , 1998 101-110

Article

Low Density Membranes Are Associated with RNA-binding Proteins and Thylakoids in the Chloroplast of Chlamydomonas reinhardtii



William Zerges and Jean-David Rochaix

Department of Molecular Biology and Department of Plant Biology, University of Geneva, 30 Quai Ernest-Ansermet, CH-1211, Geneva 4, Switzerland

Chloroplast subfractions were tested with a UV cross-linking assay for proteins that bind to the 5' untranslated region of the chloroplast psbC mRNA of the green alga Chlamydomonas reinhardtii. These analyses revealed that RNA-binding proteins of 30–32, 46, 47, 60, and 80 kD are associated with chloroplast membranes. The buoyant density and the acyl lipid composition of these membranes are compatible with their origin being the inner chloroplast envelope membrane. However, unlike previously characterized inner envelope membranes, these membranes are associated with thylakoids. One of the membrane-associated RNA-binding proteins appears to be RB47, which has been reported to be a specific activator of psbA mRNA translation. These results suggest that translation of chloroplast mRNAs encoding thylakoid proteins occurs at either a subfraction of the chloroplast inner envelope membrane or a previously uncharacterized intra-chloroplast compartment, which is physically associated with thylakoids.

Abbreviations used in this paper: DGDG, digalactosyldiacylglycerol; MGDG, monogalactosyldiacylglycerol; PG, phosphatidylglycerol; PI, phosphatidylinositol; PSI, and PSII, photosystem I and II; UTR, untranslated region.

Address all correspondence to Jean-David Rochaix, Departments of Molecular Biology and Plant Biology, University of Geneva, 30 Quai Ernest-Ansermet, CH-1211, Geneva 4, Switzerland. Tel.: 41-22-702-6187. Fax: 41-22-702-6868.

W. Zerges' present address is Developmental, Cell and Molecular Biology, LSRC, Box 91000, Duke University, Durham, NC 27708-1000. E-mail: zerges{at}acpub.duke.edu


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