Ripped Pocket and Pickpocket, Novel Drosophila DEG/ENaC Subunits Expressed in Early Development and in Mechanosensory Neurons

doi:10.1083/jcb.140.1.143

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© The Rockefeller University Press, 0021-9525/1998//143 $5.00
The Journal of Cell Biology, Volume 140, Number 1, , 1998 143-152

Article

Ripped Pocket and Pickpocket, Novel Drosophila DEG/ENaC Subunits Expressed in Early Development and in Mechanosensory Neurons

Christopher M. Adams, Michael G. Anderson, David G. Motto, Margaret P. Price, Wayne A. Johnson, and Michael J. Welsh

Howard Hughes Medical Institute, Departments of Internal Medicine and Physiology and Biophysics, University of Iowa College of Medicine, Iowa City, Iowa 52242

Drosophila melanogaster has proven to be a good model for understandingthe physiology of ion channels. We identified two novel DrosophilaDEG/ ENaC proteins, Pickpocket (PPK) and Ripped Pocket (RPK).Both appear to be ion channel subunits. Expression of RPK generatedmultimeric Na⁺ channels that were dominantly activated by amutation associated with neurodegeneration. Amiloride and gadolinium,which block mechanosensation in vivo, inhibited RPK channels.Although PPK did not form channels on its own, it associatedwith and reduced the current generated by a related human brainNa⁺ channel. RPK transcripts were abundant in early stage embryos,suggesting a role in development. In contrast, PPK was foundin sensory dendrites of a subset of peripheral neurons in latestage embryos and early larvae. In insects, such multiple dendriticneurons play key roles in touch sensation and proprioceptionand their morphology resembles human mechanosensory free nerveendings. These results suggest that PPK may be a channel subunitinvolved in mechanosensation.

Abbreviations used in this paper: BNC1, brain Na⁺ channel; CFTR, cystic fibrosis transmembrane conductance regulator; da, dendrites that arborize; DIG, digoxygenin; ENaC, epithelial Na⁺ channel; es, external sensory; md, multiple dendritic; PNS, peripheral nervous system; PPK, pickpocket; RPK, ripped pocket; SEAP, secreted alkaline phosphatase.

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