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J. Cell Biol.,
Volume 140, Number 1, January 12, 1998 29-37
Department of Biochemistry and Cell Biology, State University of New York, Stony Brook, Stony Brook, New York 11794
Spore formation in yeast is an unusual form
of cell division in which the daughter cells are formed
within the mother cell cytoplasm. This division requires
the de novo synthesis of a membrane compartment,
termed the prospore membrane, which engulfs the
daughter nuclei. The effect of mutations in late-acting
genes on sporulation was investigated. Mutation of
SEC1, SEC4, or SEC8 blocked spore formation, and
electron microscopic analysis of the sec4-8 mutant indicated that this inability to produce spores was caused
by a failure to form the prospore membrane. The soluble NSF attachment protein 25 (SNAP-25) homologue
SEC9, by contrast, was not required for sporulation.
The absence of a requirement for SEC9 was shown to
be due to the sporulation-specific induction of a second,
previously undescribed, SNAP-25 homologue, termed
SPO20. These results define a developmentally regulated branch of the secretory pathway and suggest that
spore morphogenesis in yeast proceeds by the targeting and fusion of secretory vesicles to form new plasma
membranes in the interior of the mother cell. Consistent with this model, the extracellular proteins Gas1p
and Cts1p were localized to an internal compartment in
sporulating cells. Spore formation in yeast may be a
useful model for understanding secretion-driven cell division events in a variety of plant and animal systems.
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