JCB logo
amgmicro.com
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
This Article
Right arrow Full Text
Right arrow Full Text (PDF, 582K)
Right arrow PPT slides of all figures
Right arrow Alert me when this article is cited
Right arrow Citation Map
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new content in the JCB
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Neiman, A. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Neiman, A. M.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Facebook   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

© The Rockefeller University Press, 0021-9525/1998//29 $5.00
The Journal of Cell Biology, Volume 140, Number 1, , 1998 29-37

Article

Prospore Membrane Formation Defines a Developmentally Regulated Branch of the Secretory Pathway in Yeast



Aaron M. Neiman

Department of Biochemistry and Cell Biology, State University of New York, Stony Brook, Stony Brook, New York 11794

Spore formation in yeast is an unusual form of cell division in which the daughter cells are formed within the mother cell cytoplasm. This division requires the de novo synthesis of a membrane compartment, termed the prospore membrane, which engulfs the daughter nuclei. The effect of mutations in late-acting genes on sporulation was investigated. Mutation of SEC1, SEC4, or SEC8 blocked spore formation, and electron microscopic analysis of the sec4-8 mutant indicated that this inability to produce spores was caused by a failure to form the prospore membrane. The soluble NSF attachment protein 25 (SNAP-25) homologue SEC9, by contrast, was not required for sporulation. The absence of a requirement for SEC9 was shown to be due to the sporulation-specific induction of a second, previously undescribed, SNAP-25 homologue, termed SPO20. These results define a developmentally regulated branch of the secretory pathway and suggest that spore morphogenesis in yeast proceeds by the targeting and fusion of secretory vesicles to form new plasma membranes in the interior of the mother cell. Consistent with this model, the extracellular proteins Gas1p and Cts1p were localized to an internal compartment in sporulating cells. Spore formation in yeast may be a useful model for understanding secretion-driven cell division events in a variety of plant and animal systems.

Abbreviations used in this paper: DAPI, 4',6'-diamidino-2-phenylindoledihydrochloride; HA, hemagglutinin; NSF, N-ethylmaleimide–sensitive factor; SNAP, soluble NSF attachment protein; SNARE, SNAP receptor; v- and t-SNARE, vesicular and target SNARE.

I thank N. Hollingsworth, R. Sternglanz, P. Pryciak (University of Massachusetts, Worcester, MA), N. Dean, and an anonymous reviewer for comments on the manuscript; N. Hollingsworth, C. Kaiser (Massachusetts Institute of Technology, Cambridge, MA), and D. Terbush (Yale University, New Haven, CT) for strains; and M. Conrad (Oklahoma Medical Research Foundation) for communication of results before publication. I am grateful to N. Dean for antibodies and to G. Rudomen for able assistance with the electron microscopy. I am also indebted to N. Hollingsworth, N. Dean, B. Aronson, and R. Sternglanz for discussion, advice, and encouragement throughout the course of this project.

A.M. Neiman was supported by the Cancer Research Fund of the Damon Runyon-Walter Winchell Foundation, DRG-1276 and National Institutes of Health grant GM-28220 to R. Sternglanz.

Address all correspondence to Aaron M. Neiman, Department of Biochemistry and Cell Biology, State University of New York, Stony Brook, Stony Brook, NY 11794. Tel.: (516) 632-8565. Fax: (516) 632-8575. E-mail: aneiman{at}mcbsgi.bio.sunysb.edu


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Facebook Facebook   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:



  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents