In Vitro Degradation of Endothelial Catenins by a Neutrophil Protease

doi:10.1083/jcb.140.2.403

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© The Rockefeller University Press, 0021-9525/1998//403 $5.00
The Journal of Cell Biology, Volume 140, Number 2, , 1998 403-407

Article

In Vitro Degradation of Endothelial Catenins by a Neutrophil Protease

Thomas Moll^*, Elisabetta Dejana, and Dietmar Vestweber^*

^* Institute of Cell Biology, Zentrum für die Molekularbiologie der Entzündung, University of Münster, Germany; and Istituto di Ricerche Farmacologiche Mario Negri, Milano, Italy

It has been recently proposed that adhesion of polymorphonuclearcells (PMNs) to human umbilical vein endothelial cells leadsto the disorganization of the vascular endothelial cadherin–dependentendothelial adherens junctions. Combined immunofluorescence and biochemical data suggested that after adhesion of PMNsto the endothelial cell surface, β-catenin, as well asplakoglobin was lost from the cadherin/catenin complex and fromtotal cell lysates. In this study we present data that stronglysuggest that the adhesion-dependent disappearance of endothelialcatenins is not mediated by a leukocyte to endothelium signalingevent, but is due to the activity of a neutrophil proteasethat is released upon detergent lysis of the cells.

Abbreviations used in this paper: EC, endothelial cells; HUVEC, human umbilical vein endothelial cells; PFA, paraformaldehyde; PMN, polymorphonuclear cell; VE, vascular endothelial.

Address all correspondence to D. Vestweber, Institute of CellBiology, ZMBE, Technologiehof Münster, Mendelstrasse 11,D-48149 Münster, Germany. Tel.: 49-251-835-86-17; FAX:49-251-835-86-16; E-mail:vestweb{at}uni-muenster.de

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