The Cohesion Protein MEI-S332 Localizes to Condensed Meiotic and Mitotic Centromeres until Sister Chromatids Separate

doi:10.1083/jcb.140.5.1003

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J. Cell Biol., Volume 140, Number 5, March 9, 1998 1003-1012

The Cohesion Protein MEI-S332 Localizes to Condensed Meiotic and Mitotic Centromeres until Sister Chromatids Separate

Daniel P. Moore, Andrea W. Page, Tracy Tzu-Ling Tang, Anne W. Kerrebrock, and Terry L. Orr-Weaver

Whitehead Institute for Biomedical Research, and the Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02142

The Drosophila MEI-S332 protein has been shown to be required for the maintenance of sister-chromatid cohesion in male andfemale meiosis. The protein localizes to the centromeres duringmale meiosis when the sister chromatids are attached, and it isno longer detectable after they separate. Drosophila melanogastermale meiosis is atypical in several respects, making it importantto define MEI-S332 behavior during female meiosis, which bettertypifies meiosis in eukaryotes. We find that MEI-S332 localizesto the centromeres of prometaphase I chromosomes in oocytes, remainingthere until it is delocalized at anaphase II. By using oocyteswe were able to obtain sufficient material to investigate thefate of MEI-S332 after the metaphase II-anaphase II transition.The levels of MEI-S332 protein are unchanged after the completionof meiosis, even when translation is blocked, suggesting thatthe protein dissociates from the centromeres but is not degradedat the onset of anaphase II. Unexpectedly, MEI-S332 is presentduring embryogenesis, localizes onto the centromeres of mitoticchromosomes, and is delocalized from anaphase chromosomes. Thus,MEI-S332 associates with the centromeres of both meiotic and mitoticchromosomes and dissociates from them at anaphase.

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