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J. Cell Biol.,
Volume 140, Number 5, March 9, 1998 1013-1021
Cell Biology Laboratory, Imperial Cancer Research Fund, London WC2A, 3PX, United Kingdom
We have previously shown that p115, a vesicle docking protein, binds to two proteins (p130 and
p400) in detergent extracts of Golgi membranes. p130
was identified as GM130, a Golgi matrix protein, and
was shown to act as a membrane receptor for p115. p400 has now been identified as giantin, a Golgi membrane protein with most of its mass projecting into the
cytoplasm. Giantin is found on COPI vesicles and pretreatment with antibodies inhibits both the binding of
p115 and the docking of these vesicles with Golgi membranes. In contrast, GM130 is depleted from COPI vesicles and inhibition of the GM130 on Golgi membranes,
using either antibodies or an NH2-terminal GM130
peptide, inhibits p115 binding and vesicle docking. Together these results suggest that COPI vesicles are docked by giantin on the COPI vesicles and GM130 on
Golgi membranes with p115 providing a bridge.
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