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© The Rockefeller University Press, 0021-9525/1998//1039 $5.00
The Journal of Cell Biology, Volume 140, Number 5, , 1998 1039-1053

Article

Rab17 Regulates Membrane Trafficking through Apical Recycling Endosomes in Polarized Epithelial Cells



Paola Zacchi*, Harald Stenmark{ddagger}, Robert G. Parton||, Donata Orioli*, Filip Lim§, Angelika Giner*, Ira Mellman, Marino Zerial*, and Carol Murphy*,**

* European Molecular Biology Laboratory, Postfach 10.2209, D-69012 Heidelberg, Germany; {ddagger} Department of Biochemistry, the Norwegian Radium Hospital, Montebello, N-0310 Oslo, Norway; § Centro de Biologia Molecular, Facultad de Ciencias, Universidad Autonoma de Madrid, 28049 Cantoblanco, Spain; || Centre for Microscopy and Microanalysis, Department of Physiology and Pharmacology, and Centre for Molecular and Cellular Biology, University of Queensland, 4072 Brisbane, Australia; Department of Cell Biology, Yale University School of Medicine, New Haven, Connecticut 06520; and ** Laboratory of Biological Chemistry, Medical School, University of Ioannina, 45110 Ioannina, Greece

A key feature of polarized epithelial cells is the ability to maintain the specific biochemical composition of the apical and basolateral plasma membrane domains while selectively allowing transport of proteins and lipids from one pole to the opposite by transcytosis. The small GTPase, rab17, a member of the rab family of regulators of intracellular transport, is specifically induced during cell polarization in the developing kidney. We here examined its intracellular distribution and function in both nonpolarized and polarized cells. By confocal immunofluorescence microscopy, rab17 colocalized with internalized transferrin in the perinuclear recycling endosome of BHK-21 cells. In polarized Eph4 cells, rab17 associated with the apical recycling endosome that has been implicated in recycling and transcytosis. The localization of rab17, therefore, strengthens the proposed homology between this compartment and the recycling endosome of nonpolarized cells. Basolateral to apical transport of two membrane-bound markers, the transferrin receptor and the FcLR 5-27 chimeric receptor, was specifically increased in Eph4 cells expressing rab17 mutants defective in either GTP binding or hydrolysis. Furthermore, the mutant proteins stimulated apical recycling of FcLR 5-27. These results support a role for rab17 in regulating traffic through the apical recycling endosome, suggesting a function in polarized sorting in epithelial cells.

Abbreviations used in this paper: ECL, electrochemiluminescence; hTR, human transferrin receptor; IFN, interferon; RT, room temperature; Tf, transferrin; TR, transferrin receptor; WT, wild type.

Address all correspondence to Carol Murphy, Laboratory of Biological Chemistry, Medical School, University of Ioannina, 45110 Ioannina, Greece. Tel.: 30 651 97560. Fax: 30 651 67868. E-mail: cmurphy{at}cc.uoi.gr

Marino Zerial, European Molecular Biology Laboratory, Postfach 10.2209, 69012 Heidelberg, Germany. Tel.: 49 6221 387 232. Fax: 49 6221 387 306. E-mail: zerial{at}embl-heidelberg.de


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