Vac8p, a Vacuolar Protein with Armadillo Repeats, Functions in both Vacuole Inheritance and Protein Targeting from the Cytoplasm to Vacuole

doi:10.1083/jcb.140.5.1063

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J. Cell Biol., Volume 140, Number 5, March 9, 1998 1063-1074

Vac8p, a Vacuolar Protein with Armadillo Repeats, Functions in both Vacuole Inheritance and Protein Targeting from the Cytoplasm to Vacuole

Yong-Xu Wang, Natalie L. Catlett, and Lois S. Weisman

Department of Biochemistry, University of Iowa, Iowa City, Iowa 52242

During each cell cycle, the yeast vacuole actively partitions between mother and daughter cells. This process requires actin,profilin, an unconventional myosin (Myo2p), and Vac8p. A mutantyeast strain, vac8, is defective in vacuole inheritance, specifically,in early vacuole migration. Vac8p is a 64-kD protein found onthe vacuole membrane, a site consistent with its role in vacuoleinheritance. Both myristoylation and palmitoylation are requiredfor complete Vac8p localization. Interestingly, whereas myristoylationof Vac8p is not required for vacuole inheritance, palmitoylationis essential. Thus, palmitoylation appears to play a more directrole in vacuole inheritance. Most of the VAC8 sequence encodes11 armadillo (Arm) repeats. Arm repeats are thought to mediateprotein-protein interactions, and many Arm proteins have multiplefunctions. This is also true for Vac8p. In addition to its rolein early vacuole inheritance, Vac8p is required to target aminopeptidaseI from the cytoplasm to the vacuole. Mutant analysis demonstratesthat Vac8p functions separately in these two processes. Vac8pcosediments with actin filaments. Vac8p is related to $beta$ -cateninand plakoglobin, which connect a specific region of the plasmamembrane to the actin cytoskeleton. In analogy, Vac8p may linkthe vacuole to actin during vacuole partitioning.

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