Vac8p, a Vacuolar Protein with Armadillo Repeats, Functions in both Vacuole Inheritance and Protein Targeting from the Cytoplasm to Vacuole

doi:10.1083/jcb.140.5.1063

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© The Rockefeller University Press, 0021-9525/1998//1063 $5.00
The Journal of Cell Biology, Volume 140, Number 5, , 1998 1063-1074

Article

Vac8p, a Vacuolar Protein with Armadillo Repeats, Functions in both Vacuole Inheritance and Protein Targeting from the Cytoplasm to Vacuole

Yong-Xu Wang, Natalie L. Catlett, and Lois S. Weisman

Department of Biochemistry, University of Iowa, Iowa City, Iowa 52242

During each cell cycle, the yeast vacuole actively partitionsbetween mother and daughter cells. This process requires actin,profilin, an unconventional myosin (Myo2p), and Vac8p. A mutantyeast strain, vac8, is defective in vacuole inheritance, specifically,in early vacuole migration. Vac8p is a 64-kD protein foundon the vacuole membrane, a site consistent with its role invacuole inheritance. Both myristoylation and palmitoylationare required for complete Vac8p localization. Interestingly,whereas myristoylation of Vac8p is not required for vacuoleinheritance, palmitoylation is essential. Thus, palmitoylationappears to play a more direct role in vacuole inheritance.Most of the VAC8 sequence encodes 11 armadillo (Arm) repeats. Arm repeats are thought to mediate protein–protein interactions,and many Arm proteins have multiple functions. This is alsotrue for Vac8p. In addition to its role in early vacuole inheritance,Vac8p is required to target aminopeptidase I from the cytoplasmto the vacuole. Mutant analysis demonstrates that Vac8p functions separately in these two processes. Vac8p cosediments withactin filaments. Vac8p is related to β-catenin and plakoglobin,which connect a specific region of the plasma membrane to theactin cytoskeleton. In analogy, Vac8p may link the vacuole toactin during vacuole partitioning.

Abbreviations used in this paper: API, aminopeptidase I; Arm, armadillo; CPY, carboxypeptidase Y; Cvt, cytoplasm to vacuole targeting; Vac, vacuole segregation; WT, wild-type.

L.S. Weisman is a Carver Research Scientist. This work was supported by a gift to L.S. Weisman from the Roy J. Carver CharitableTrust, and National Institutes of Health grant GM50403 to L.S.Weisman.

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