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© The Rockefeller University Press,
0021-9525/1998//1101 $5.00
The Journal of Cell Biology, Volume 140, Number 5,
, 1998 1101-1111
Article |
Differential Targeting of Vesicular Stomatitis Virus G Protein and Influenza Virus Hemagglutinin Appears During Myogenesis of L6 Muscle Cells
Exocytic organelles undergo profound reorganization during myoblast differentiation and fusion. Here, we analyzed whether glycoprotein processing and targeting changed during this process by using vesicular stomatitis virus (VSV) G protein and influenza virus hemagglutinin (HA) as models. After the induction of differentiation, the maturation and transport of the VSV G protein changed dramatically. Thus, only half of the G protein was processed and traveled through the Golgi, whereas the other half remained unprocessed. Experiments with the VSV tsO45 mutant indicated that the unprocessed form folded and trimerized normally and then exited the ER. It did not, however, travel through the Golgi since brefeldin A recalled it back to the ER. Influenza virus HA glycoprotein, on the contrary, acquired resistance to endoglycosidase H and insolubility in Triton X-100, indicating passage through the Golgi. Biochemical and morphological assays indicated that the HA appeared at the myotube surface. A major fraction of the Golgi-processed VSV G protein, however, did not appear at the myotube surface, but was found in intracellular vesicles that partially colocalized with the regulatable glucose transporter. Taken together, the results suggest that, during early myogenic differentiation, the VSV G protein was rerouted into developing, muscle-specific membrane compartments. Influenza virus HA, on the contrary, was targeted to the myotube surface.
Abbreviations used in this paper: endo H, endoglycosidase H; HA, hemagglutinin; SFV, Semliki Forest virus; SR, sarcoplasmic reticulum; T, transverse; VSV, vesicular stomatitis virus.
Address all correspondence to Kalervo Metsikkö, Department of Anatomy, University of Oulu, Kajaanintie 52A, FIN-90220 Oulu, Finland. Tel.: (358) 853-75-183. Fax: (358) 853-75-172. E-mail: metsikko{at}sun3.oulu.fi
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