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© The Rockefeller University Press, 0021-9525/1998//1137 $5.00
The Journal of Cell Biology, Volume 140, Number 5, , 1998 1137-1147

Article

Identification of the t Complex–encoded Cytoplasmic Dynein Light Chain Tctex1 in Inner Arm I1 Supports the Involvement of Flagellar Dyneins in Meiotic Drive



Alistair Harrison*, Patricia Olds-Clarke{ddagger}, and Stephen M. King*

* Department of Biochemistry, University of Connecticut Health Center, Farmington, Connecticut 06032-3305, and {ddagger} Department of Anatomy and Cell Biology, Temple University School of Medicine, Philadelphia, Pennsylvania 19140

The cytoplasmic dynein light chain Tctex1 is a candidate for one of the distorter products involved in the non-Mendelian transmission of mouse t haplotypes. It has been unclear, however, how the t-specific mutations in this protein, which is found associated with cytoplasmic dynein in many tissues, could result in a male germ cell–specific phenotype. Here, we demonstrate that Tctex1 is not only a cytoplasmic dynein component, but is also present both in mouse sperm and Chlamydomonas flagella. Genetic and biochemical dissection of the Chlamydomonas flagellum reveal that Tctex1 is a previously undescribed component of inner dynein arm I1. Combined with the recent identification of another putative t complex distorter, Tctex2, within the outer dynein arm, these results support the hypothesis that transmission ratio distortion (meiotic drive) of mouse t haplotypes involves dysfunction of both flagellar inner and outer dynein arms but does not require the cytoplasmic isozyme.

Abbreviations used in this paper: HC, heavy chain; IC, intermediate chain; LC, light chain; LIC, light intermediate chain; PVDF, polyvinylidene difluoride.

Address all correspondence to Stephen M. King, Department of Biochemistry, University of Connecticut Health Center, 263 Farmington Avenue, Farmington, CT 06032-3305. Tel.: (860) 679-3347. Fax: (860) 679-3408. E-mail: king{at}panda.uchc.edu

3. tx/ty indicates mice that carry t haplotypes with different embryonic lethal factors that do not affect sperm function (Silver, 1993).

2. Because of a recent revision in the nomenclature of murine genes, the tctex2 gene has been formally reassigned as tcte3 (see the Mouse Genome Database on the World Wide Web at www.informatics.jax.org). We have retained the Tctex2 designation here, however, to allow for continuity with previous reports.


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