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© The Rockefeller University Press, 0021-9525/1998//991 $5.00
The Journal of Cell Biology, Volume 140, Number 5, , 1998 991-1002

Article

A Dominant Mutant of Inner Centromere Protein (INCENP), a Chromosomal Protein, Disrupts Prometaphase Congression and Cytokinesis



Alastair M. Mackay*, Alexandra M. Ainsztein*,{ddagger}, D. Mark Eckley*,{ddagger}, and William C. Earnshaw{ddagger}

* Department of Cell Biology and Anatomy, Johns Hopkins School of Medicine, Baltimore, Maryland 21205; and {ddagger} Institute of Cell and Molecular Biology, University of Edinburgh, Edinburgh EH9 3JR, Scotland, United Kindgom

INCENP is a tightly bound chromosomal protein that transfers to the spindle midzone at the metaphase/anaphase transition. Here, we show that an INCENP truncation mutant (INCENP382–839) associates with microtubules but does not bind to chromosomes, and coats the entire spindle throughout mitosis. Furthermore, an INCENP truncation mutant (INCENP43–839) previously shown not to transfer to the spindle at anaphase (Mackay, A.M., D.M. Eckley, C. Chue, and W.C. Earnshaw. 1993. J. Cell Biol. 123:373–385), is shown here to bind chromosomes, but is unable to target to the centromere. Thus, association with the chromosomes, and specifically with centromeres, appears to be essential for INCENP targeting to the correct spindle subdomain at anaphase. An INCENP truncation mutant (INCENP1–405) that targets to centromeres but lacks the microtubule association region acquires strong dominant-negative characteristics. INCENP1–405 interferes with both prometaphase chromosome alignment and the completion of cytokinesis. INCENP1–405 apparently exerts its effect by displacing the endogenous protein from centromeres. These experiments provide evidence of an unexpected link between this chromosomal protein and cytokinesis, and suggest that one function of INCENP may be to integrate the chromosomal and cytoskeletal events of mitosis.

Abbreviations used in this paper: TD-60, telophase disk protein of 60 kD; DAPI, 4,6-diamidino-Z-phenylindole; GFP, green fluorescent protein; INCENP, inner centromere protein; CENP-E, centromere protein E.

A.M. Mackay's present address is Osiris Therapeutics, 2001 Aliceanna St., Baltimore, MD 21231.

Address all correspondence to William C. Earnshaw, Institute of Cell and Molecular Biology, University of Edinburgh, Swann Building, The King's Buildings, Mayfield Road, Edinburgh EH9 3JR Scotland, United Kingdom. Tel.: (44) 131-650-7101. Fax: (44) 131-650-7100. E-mail: bill. earnshaw{at}ed.ac.uk


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