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© The Rockefeller University Press, 0021-9525/1998//143 $5.00
The Journal of Cell Biology, Volume 141, Number 1, , 1998 143-153

The 13-kD FK506 Binding Protein, FKBP13, Interacts with a Novel Homologue of the Erythrocyte Membrane Cytoskeletal Protein 4.1

Loren D. Walensky^*,, Philippe Gascard^||, Michael E. Field^*, Seth Blackshaw^*, John G. Conboy^||, Narla Mohandas^||, and Solomon H. Snyder^*,,

^* Department of Neuroscience, Department of Pharmacology and Molecular Sciences, and Department of Psychiatry and Behavioral Sciences, The Johns Hopkins University School of Medicine, Baltimore, Maryland, 21205; and ^|| Life Sciences Division, Lawrence Berkeley National Laboratory, University of California, Berkeley, CA 94720

We have identified a novel generally expressed homologue of the erythrocyte membrane cytoskeletal protein 4.1, named 4.1G, based on the interaction of its COOH-terminal domain (CTD) with the immunophilin FKBP13. The 129-amino acid peptide, designated 4.1G–CTD, is the first known physiologic binding target of FKBP13. FKBP13 is a 13-kD protein originally identified by its high affinity binding to the immunosuppressant drugs FK506 and rapamycin (Jin, Y., M.W. Albers, W.S. Lane, B.E. Bierer, and S.J. Burakoff. 1991. Proc. Natl. Acad. Sci. USA. 88:6677– 6681); it is a membrane-associated protein thought to function as an ER chaperone (Bush, K.T., B.A. Henrickson, and S.K. Nigam. 1994. Biochem. J. [Tokyo]. 303:705–708). We report the specific association of FKBP13 with 4.1G–CTD based on yeast two-hybrid, in vitro binding and coimmunoprecipitation experiments. The histidyl-proline moiety of 4.1G–CTD is required for FKBP13 binding, as indicated by yeast experiments with truncated and mutated 4.1G–CTD constructs. In situ hybridization studies reveal cellular colocalizations for FKBP13 and 4.1G–CTD throughout the body during development, supporting a physiologic role for the interaction. Interestingly, FKBP13 cofractionates with the red blood cell homologue of 4.1 (4.1R) in ghosts, inside-out vesicles, and Triton shell preparations. The identification of FKBP13 in erythrocytes, which lack ER, suggests that FKBP13 may additionally function as a component of membrane cytoskeletal scaffolds.

Abbreviations used in this paper: 4.1G, general protein 4.1; 4.1R, red blood cell protein 4.1; aa, amino acid(s); ala, alanine; arg, arginine; β-gal, β-galactosidase; CTD, COOH-terminal domain; EST, expressed sequence tag; FKBP, FK506 binding protein; GAL4(DB), GAL4 DNA–binding domain; GAL4(TA), GAL4 transactivating domain; gln, glutamine; HA, hemagglutinin; his, histidine; h,m,r4.1G, human, mouse, rat 4.1G; h,m,r4.1R, human, mouse, rat 4.1R; ile, isoleucine; IOV, inside-out vesicle; IP₃, inositol 1,4,5-trisphosphate; leu, leucine; MBD, membrane-binding domain; PEG, polyethylene glycol; pro, proline; PVDF, polyvinylene difluoride; RBC, red blood cell; RT, reverse transcription; SABD, spectrin actin-binding domain; ser, serine; trp, tryptophan; val, valine.

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