Processing of Laminin-5 and Its Functional Consequences: Role of Plasmin and Tissue-type Plasminogen Activator

doi:10.1083/jcb.141.1.255

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© The Rockefeller University Press, 0021-9525/1998//255 $5.00
The Journal of Cell Biology, Volume 141, Number 1, , 1998 255-265

Regular Articles

Processing of Laminin-5 and Its Functional Consequences: Role of Plasmin and Tissue-type Plasminogen Activator

Lawrence E. Goldfinger^*, M. Sharon Stack^*^,, and Jonathan C.R. Jones^*

^* Department of Cell and Molecular Biology, and Department of Obstetrics and Gynecology, Northwestern University Medical School, Chicago, Illinois 60611

The laminin-5 component of the extracellular matrices of certaincultured cells such as the rat epithelial cell line 804G andthe human breast epithelial cell MCF-10A is capable of nucleatingassembly of cell– matrix adhesive devices called hemidesmosomeswhen other cells are plated upon them. These matrices also impede cell motility. In contrast, cells plated onto the laminin-5–richmatrices of pp126 epithelial cells fail to assemble hemidesmosomesand are motile. To understand these contradictory phenomena,we have compared the forms of heterotrimeric laminin-5 secretedby 804G and MCF-10A cells with those secreted by pp126 cells,using a panel of laminin-5 subunit-specific antibodies. The ${alpha}$ 3 subunit of laminin-5 secreted by pp126 cells migrates at190 kD, whereas that secreted by 804G and MCF-10A cells migratesat 160 kD. The pp126 cell 190-kD ${alpha}$ 3 chain of laminin-5 can bespecifically proteolyzed by plasmin to a 160-kD species at enzymeconcentrations that do not apparently effect the laminin-5 β and ${gamma}$ chains. After plasmin treatment, pp126 cell laminin-5not only impedes cell motility but also becomes competent tonucleate assembly of hemidesmosomes. The possibility that plasminmay play an important role in processing laminin-5 subunitsis supported by immunofluorescence analyses that demonstrate colocalization of laminin-5 and plasminogen in the extracellularmatrix of MCF-10A and pp126 cells. Whereas tissue-type plasminogenactivator (tPA), which converts plasminogen to plasmin, codistributes with laminin-5 in MCF-10A matrix, tPA is not present in pp126extracellular matrix. Treatment of pp126 laminin-5–richextracellular matrix with exogenous tPA results in proteolysisof the laminin-5 ${alpha}$ 3 chain from 190 to 160 kD. In addition, plasminogenand tPA bind laminin-5 in vitro. In summary, we provide evidencethat laminin-5 is a multifunctional protein that can act under certain circumstances as a motility and at other times as an adhesive factor. In cells in culture, this functional conversionappears dependent upon and is regulated by tPA and plasminogen.

Abbreviations used in this paper: ECM, extracellular matrix; His, histidine; MMP, matrix metalloproteinase; NHEK, normal human keratinocyte; SSC, squamous cell carcinoma; tPA, tissue-type plasminogen activator; uPA, urinary-type plasminogen activator.

Address all correspondence to Jonathan C.R. Jones, Departmentof Cell and Molecular Biology, Morton 4-616, Northwestern UniversitySchool of Medicine, 303 East Chicago Avenue, Chicago, IL 60611.Tel.: (312) 503-1412. Fax: (312) 503-6475. E-mail: j-jones3{at}nwu.edu

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Krampert, M., Bloch, W., Sasaki, T., Bugnon, P., Rulicke, T., Wolf, E., Aumailley, M., Parks, W. C., Werner, S. (2004). Activities of the Matrix Metalloproteinase Stromelysin-2 (MMP-10) in Matrix Degradation and Keratinocyte Organization in Wounded Skin. Mol. Biol. Cell 15: 5242-5254 [Abstract] [Full Text]
Sigle, R. O., Gil, S. G., Bhattacharya, M., Ryan, M. C., Yang, T.-M., Brown, T. A., Boutaud, A., Miyashita, Y., Olerud, J., Carter, W. G. (2004). Globular domains 4/5 of the laminin {alpha}3 chain mediate deposition of precursor laminin 5. J. Cell Sci. 117: 4481-4494 [Abstract] [Full Text]
Tasanen, K., Tunggal, L., Chometon, G., Bruckner-Tuderman, L., Aumailley, M. (2004). Keratinocytes from Patients Lacking Collagen XVII Display a Migratory Phenotype. Am. J. Pathol. 164: 2027-2038 [Abstract] [Full Text]
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Frank, D. E., Carter, W. G. (2004). Laminin 5 deposition regulates keratinocyte polarization and persistent migration. J. Cell Sci. 117: 1351-1363 [Abstract] [Full Text]
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Joly, D., Morel, V., Hummel, A., Ruello, A., Nusbaum, P., Patey, N., Noel, L.-H., Rousselle, P., Knebelmann, B. (2003). {beta}4 Integrin and Laminin 5 Are Aberrantly Expressed in Polycystic Kidney Disease: Role in Increased Cell Adhesion and Migration. Am. J. Pathol. 163: 1791-1800 [Abstract] [Full Text]
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Solberg, H., Rinkenberger, J., Dano, K., Werb, Z., Lund, L. R. (2003). A functional overlap of plasminogen and MMPs regulates vascularization during placental development. Development 130: 4439-4450 [Abstract] [Full Text]
Utani, A., Momota, Y., Endo, H., Kasuya, Y., Beck, K., Suzuki, N., Nomizu, M., Shinkai, H. (2003). Laminin {alpha}3 LG4 Module Induces Matrix Metalloproteinase-1 through Mitogen-activated Protein Kinase Signaling. J. Biol. Chem. 278: 34483-34490 [Abstract] [Full Text]
Natarajan, E., Saeb, M., Crum, C. P., Woo, S. B., McKee, P. H., Rheinwald, J. G. (2003). Co-Expression of p16INK4A and Laminin 5 {gamma}2 by Microinvasive and Superficial Squamous Cell Carcinomas in Vivo and by Migrating Wound and Senescent Keratinocytes in Culture. Am. J. Pathol. 163: 477-491 [Abstract] [Full Text]
Udayakumar, T. S., Chen, M. L., Bair, E. L., von Bredow, D. C., Cress, A. E., Nagle, R. B., Bowden, G. T. (2003). Membrane Type-1-Matrix Metalloproteinase Expressed by Prostate Carcinoma Cells Cleaves Human Laminin-5 {beta}3 Chain and Induces Cell Migration. Cancer Res. 63: 2292-2299 [Abstract] [Full Text]
Veitch, D. P., Nokelainen, P., McGowan, K. A., Nguyen, T.-T., Nguyen, N. E., Stephenson, R., Pappano, W. N., Keene, D. R., Spong, S. M., Greenspan, D. S., Findell, P. R., Marinkovich, M. P. (2003). Mammalian Tolloid Metalloproteinase, and Not Matrix Metalloprotease 2 or Membrane Type 1 Metalloprotease, Processes Laminin-5 in Keratinocytes and Skin. J. Biol. Chem. 278: 15661-15668 [Abstract] [Full Text]
Hirako, Y., Yoshino, K., Zillikens, D., Owaribe, K. (2003). Extracellular Cleavage of Bullous Pemphigoid Antigen 180/Type XVII Collagen and Its Involvement in Hemidesmosomal Disassembly. J Biochem 133: 197-206 [Abstract] [Full Text]
Hirosaki, T., Tsubota, Y., Kariya, Y., Moriyama, K., Mizushima, H., Miyazaki, K. (2002). Laminin-6 Is Activated by Proteolytic Processing and Regulates Cellular Adhesion and Migration Differently from Laminin-5. J. Biol. Chem. 277: 49287-49295 [Abstract] [Full Text]
Deugnier, M.-A., Faraldo, M. M., Janji, B., Rousselle, P., Thiery, J. P., Glukhova, M. A. (2002). EGF controls the in vivo developmental potential of a mammary epithelial cell line possessing progenitor properties. JCB 159: 453-463 [Abstract] [Full Text]
Zuk, A., Matlin, K. S. (2002). Induction of a laminin isoform and alpha 3beta 1-integrin in renal ischemic injury and repair in vivo. Am. J. Physiol. Renal Physiol. 283: F971-F984 [Abstract] [Full Text]
Geuijen, C. A. W., Sonnenberg, A. (2002). Dynamics of the alpha 6beta 4 Integrin in Keratinocytes. Mol. Biol. Cell 13: 3845-3858 [Abstract] [Full Text]
Rosmann, S., Hahn, D., Lottaz, D., Kruse, M.-N., Stocker, W., Sterchi, E. E. (2002). Activation of Human Meprin-alpha in a Cell Culture Model of Colorectal Cancer Is Triggered by the Plasminogen-activating System. J. Biol. Chem. 277: 40650-40658 [Abstract] [Full Text]
Esco, M. A., Wang, Z., McDermott, M. L., Kurpakus-Wheater, M. (2002). Potential role for laminin 5 in hypoxia-mediated apoptosis of human corneal epithelial cells. J. Cell Sci. 114: 4033-4040 [Abstract] [Full Text]
Gilles, C., Polette, M., Coraux, C., Tournier, J.-M., Meneguzzi, G., Munaut, C., Volders, L., Rousselle, P., Birembaut, P., Foidart, J.-M. (2002). Contribution of MT1-MMP and of human laminin-5 {gamma}2 chain degradation to mammary epithelial cell migration. J. Cell Sci. 114: 2967-2976 [Abstract] [Full Text]
Niki, T., Kohno, T., Iba, S., Moriya, Y., Takahashi, Y., Saito, M., Maeshima, A., Yamada, T., Matsuno, Y., Fukayama, M., Yokota, J., Hirohashi, S. (2002). Frequent Co-Localization of Cox-2 and Laminin-5 {gamma}2 Chain at the Invasive Front of Early-Stage Lung Adenocarcinomas. Am. J. Pathol. 160: 1129-1141 [Abstract] [Full Text]
Tunggal, L., Ravaux, J., Pesch, M., Smola, H., Krieg, T., Gaill, F., Sasaki, T., Timpl, R., Mauch, C., Aumailley, M. (2002). Defective Laminin 5 Processing in Cylindroma Cells. Am. J. Pathol. 160: 459-468 [Abstract] [Full Text]
Stepp, M. A., Gibson, H. E., Gala, P. H., Iglesia, D. D. Sta., Pajoohesh-Ganji, A., Pal-Ghosh, S., Brown, M., Aquino, C., Schwartz, A. M., Goldberger, O., Hinkes, M. T., Bernfield, M. (2002). Defects in keratinocyte activation during wound healing in the syndecan-1-deficient mouse. J. Cell Sci. 115: 4517-4531 [Abstract] [Full Text]
Nguyen, B. P., Ren, X.-D., Schwartz, M. A., Carter, W. G. (2001). Ligation of Integrin alpha 3beta 1 by Laminin 5 at the Wound Edge Activates Rho-dependent Adhesion of Leading Keratinocytes on Collagen. J. Biol. Chem. 276: 43860-43870 [Abstract] [Full Text]
LAPLANTE, A. F., GERMAIN, L., AUGER, F. A., MOULIN, V. (2001). Mechanisms of wound reepithelialization: hints from a tissue-engineered reconstructed skin to long-standing questions. FASEB J. 15: 2377-2389 [Abstract] [Full Text]
Salvador, L. M., Park, Y., Cottom, J., Maizels, E. T., Jones, J. C. R., Schillace, R. V., Carr, D. W., Cheung, P., Allis, C. D., Jameson, J. L., Hunzicker-Dunn, M. (2001). Follicle-stimulating Hormone Stimulates Protein Kinase A-mediated Histone H3 Phosphorylation and Acetylation Leading to Select Gene Activation in Ovarian Granulosa Cells. J. Biol. Chem. 276: 40146-40155 [Abstract] [Full Text]
Martin, S. S., Leder, P. (2001). Human MCF10A Mammary Epithelial Cells Undergo Apoptosis following Actin Depolymerization That Is Independent of Attachment and Rescued by Bcl-2. Mol. Cell. Biol. 21: 6529-6536 [Abstract] [Full Text]
Gagnoux-Palacios, L., Allegra, M., Spirito, F., Pommeret, O., Romero, C., Ortonne, J.-p., Meneguzzi, G. (2001). The Short Arm of the Laminin {gamma}2 Chain Plays a Pivotal Role in the Incorporation of Laminin 5 into the Extracellular Matrix and in Cell Adhesion. JCB 153: 835-850 [Abstract] [Full Text]
Hintermann, E., Bilban, M., Sharabi, A., Quaranta, V. (2001). Inhibitory Role of {alpha}6{beta}4-Associated Erbb-2 and Phosphoinositide 3-Kinase in Keratinocyte Haptotactic Migration Dependent on {alpha}3{beta}1 Integrin. JCB 153: 465-478 [Abstract] [Full Text]
Gonzalez, A. M., Otey, C., Edlund, M., Jones, J. C. R. (2001). Interactions of a hemidesmosome component and actinin family members. J. Cell Sci. 114: 4197-4206 [Abstract] [Full Text]
Decline, F, Rousselle, P (2001). Keratinocyte migration requires alpha2beta1 integrin-mediated interaction with the laminin 5 gamma2 chain. J. Cell Sci. 114: 811-823 [Abstract]
Steffensen, B., Hakkinen, L., Larjava, H. (2001). Proteolytic Events of Wound-Healing -- Coordinated Interactions Among Matrix Metalloproteinases (MMPs), Integrins, and Extracellular Matrix Molecules. CROBM 12: 373-398 [Abstract] [Full Text]
Gonzales, M., Weksler, B., Tsuruta, D., Goldman, R. D., Yoon, K. J., Hopkinson, S. B., Flitney, F. W., Jones, J. C. R. (2001). Structure and Function of a Vimentin-associated Matrix Adhesion in Endothelial Cells. Mol. Biol. Cell 12: 85-100 [Abstract] [Full Text]
Sterk, L. M.Th., Geuijen, C. A.W., Oomen, L. C.J.M., Calafat, J., Janssen, H., Sonnenberg, A. (2000). The Tetraspan Molecule Cd151, a Novel Constituent of Hemidesmosomes, Associates with the Integrin {alpha}6{beta}4 and May Regulate the Spatial Organization of Hemidesmosomes. JCB 149: 969-982 [Abstract] [Full Text]
Davis, G. E., Bayless, K. J., Davis, M. J., Meininger, G. A. (2000). Regulation of Tissue Injury Responses by the Exposure of Matricryptic Sites within Extracellular Matrix Molecules. Am. J. Pathol. 156: 1489-1498 [Abstract] [Full Text]
Michelson, P. H., Tigue, M., Jones, J. C.R. (2000). Human Bronchial Epithelial Cells Secrete Laminin 5, Express Hemidesmosomal Proteins, and Assemble Hemidesmosomes. J. Histochem. Cytochem. 48: 535-544 [Abstract] [Full Text]
Iglesia, D. D. Sta., Stepp, M. A. (2000). Disruption of the Basement Membrane after Corneal Debridement. IOVS 41: 1045-1053 [Abstract] [Full Text]
Miller, K. A., Chung, J., Lo, D., Jones, J. C. R., Thimmapaya, B., Weitzman, S. A. (2000). Inhibition of Laminin-5 Production in Breast Epithelial Cells by Overexpression of p300. J. Biol. Chem. 275: 8176-8182 [Abstract] [Full Text]
Koshikawa, N., Giannelli, G., Cirulli, V., Miyazaki, K., Quaranta, V. (2000). Role of Cell Surface Metalloprotease Mt1-Mmp in Epithelial Cell Migration over Laminin-5. JCB 148: 615-624 [Abstract] [Full Text]
DiPersio, C., Shao, M, Di Costanzo, L, Kreidberg, J., Hynes, R. (2000). Mouse keratinocytes immortalized with large T antigen acquire alpha3beta1 integrin-dependent secretion of MMP-9/gelatinase B. J. Cell Sci. 113: 2909-2921 [Abstract]
Son, Y.-J., Scranton, T. W., Sunderland, W. J., Baek, S. J., Miner, J. H., Sanes, J. R., Carlson, S. S. (2000). The Synaptic Vesicle Protein SV2 Is Complexed with an alpha 5-Containing Laminin on the Nerve Terminal Surface. J. Biol. Chem. 275: 451-460 [Abstract] [Full Text]
Ryan, M. C., Lee, K., Miyashita, Y., Carter, W. G. (1999). Targeted Disruption of the LAMA3 Gene in Mice Reveals Abnormalities in Survival and Late Stage Differentiation of Epithelial Cells. JCB 145: 1309-1324 [Abstract] [Full Text]
Gonzales, M., Haan, K., Baker, S. E., Fitchmun, M., Todorov, I., Weitzman, S., Jones, J. C.R. (1999). A Cell Signal Pathway Involving Laminin-5, alpha 3beta 1 Integrin, and Mitogen-activated Protein Kinase Can Regulate Epithelial Cell Proliferation. Mol. Biol. Cell 10: 259-270 [Abstract] [Full Text]
Grassi, M, Moens, G, Rousselle, P, Thiery, J., Jouanneau, J (1999). The SFL activity secreted by metastatic carcinoma cells is related to laminin 5 and mediates cell scattering in an integrin-independent manner. J. Cell Sci. 112: 2511-2520 [Abstract]
Goldfinger, L., Hopkinson, S., deHart, G., Collawn, S, Couchman, J., Jones, J. (1999). The alpha3 laminin subunit, alpha6beta4 and alpha3beta1 integrin coordinately regulate wound healing in cultured epithelial cells and in the skin. J. Cell Sci. 112: 2615-2629 [Abstract]
Lampe, P. D., Nguyen, B. P., Gil, S., Usui, M., Olerud, J., Takada, Y., Carter, W. G. (1998). Cellular Interaction of Integrin {alpha}3{beta}1 with Laminin 5 Promotes Gap Junctional Communication. JCB 143: 1735-1747 [Abstract] [Full Text]
Ghosh, S., Brown, R., Jones, J. C. R., Ellerbroek, S. M., Stack, M. S. (2000). Urinary-type Plasminogen Activator (uPA) Expression and uPA Receptor Localization Are Regulated by alpha 3beta 1 Integrin in Oral Keratinocytes. J. Biol. Chem. 275: 23869-23876 [Abstract] [Full Text]
Hirosaki, T., Mizushima, H., Tsubota, Y., Moriyama, K., Miyazaki, K. (2000). Structural Requirement of Carboxyl-terminal Globular Domains of Laminin alpha 3 Chain for Promotion of Rapid Cell Adhesion and Migration by Laminin-5. J. Biol. Chem. 275: 22495-22502 [Abstract] [Full Text]
Nguyen, B. P., Gil, S. G., Carter, W. G. (2000). Deposition of Laminin 5 by Keratinocytes Regulates Integrin Adhesion and Signaling. J. Biol. Chem. 275: 31896-31907 [Abstract] [Full Text]
Goldfinger, L. E., Jiang, L., Hopkinson, S. B., Stack, M. S., Jones, J. C. R. (2000). Spatial Regulation and Activity Modulation of Plasmin by High Affinity Binding to the G domain of the alpha 3 Subunit of Laminin-5. J. Biol. Chem. 275: 34887-34893 [Abstract] [Full Text]
Dans, M., Gagnoux-Palacios, L., Blaikie, P., Klein, S., Mariotti, A., Giancotti, F. G. (2001). Tyrosine Phosphorylation of the beta 4 Integrin Cytoplasmic Domain Mediates Shc Signaling to Extracellular Signal-regulated Kinase and Antagonizes Formation of Hemidesmosomes. J. Biol. Chem. 276: 1494-1502 [Abstract] [Full Text]
Utani, A., Nomizu, M., Matsuura, H., Kato, K., Kobayashi, T., Takeda, U., Aota, S., Nielsen, P. K., Shinkai, H. (2001). A Unique Sequence of the Laminin alpha 3 G Domain Binds to Heparin and Promotes Cell Adhesion through Syndecan-2 and -4. J. Biol. Chem. 276: 28779-28788 [Abstract] [Full Text]
Robbins, P. B., Lin, Q., Goodnough, J. B., Tian, H., Chen, X., Khavari, P. A. (2001). In vivo restoration of laminin 5 beta 3 expression and function in junctional epidermolysis bullosa. Proc. Natl. Acad. Sci. USA 98: 5193-5198 [Abstract] [Full Text]
Mukherjee, S., Gurevich, V. V., Jones, J. C. R., Casanova, J. E., Frank, S. R., Maizels, E. T., Bader, M.-F., Kahn, R. A., Palczewski, K., Aktories, K., Hunzicker-Dunn, M. (2000). The ADP ribosylation factor nucleotide exchange factor ARNO promotes beta -arrestin release necessary for luteinizing hormone/choriogonadotropin receptor desensitization. Proc. Natl. Acad. Sci. USA 97: 5901-5906 [Abstract] [Full Text]