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© The Rockefeller University Press, 0021-9525/1998//455 $5.00
The Journal of Cell Biology, Volume 141, Number 2, , 1998 455-467

Drosophila Centrosomin Protein is Required for Male Meiosis and Assembly of the Flagellar Axoneme

Howard Hughes Medical Institute, Department of Biology, Indiana University, Bloomington, IN 47405

Centrosomes and microtubules play crucial roles during cell division and differentiation. Spermatogenesis is a useful system for studying centrosomal function since it involves both mitosis and meiosis, and also transformation of the centriole into the sperm basal body. Centrosomin is a protein localized to the mitotic centrosomes in Drosophila melanogaster. We have found a novel isoform of centrosomin expressed during spermatogenesis. Additionally, an anticentrosomin antibody labels both the mitotic and meiotic centrosomes as well as the basal body. Mutational analysis shows that centrosomin is required for spindle organization during meiosis and for organization of the sperm axoneme. These results suggest that centrosomin is a necessary component of the meiotic centrosomes and the spermatid basal body.

Abbreviations used in this paper: MTOC, microtubule-organizing center; PCM, pericentriolar material.

The current address of K. Li is Beckman Center, Rm. B265, Stanford University Medical Center, Palo Alto, CA 94303-5428

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