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J. Cell Biol.,
Volume 141, Number 4, May 18, 1998 993-1008
* Molecular, Cellular and Developmental Biology, Yale University, New Haven, Connecticut 06520-8103; We previously described a kinesin-dependent movement of particles in the flagella of Chlamydomonas reinhardtii called intraflagellar transport
(IFT) (Kozminski, K.G., K.A. Johnson, P. Forscher,
and J.L. Rosenbaum. 1993. Proc. Natl. Acad. Sci. USA.
90:5519-5523). When IFT is inhibited by inactivation of
a kinesin, FLA10, in the temperature-sensitive mutant,
fla10, existing flagella resorb and new flagella cannot be
assembled. We report here that: (a) the IFT-associated
FLA10 protein is a subunit of a heterotrimeric kinesin;
(b) IFT particles are composed of 15 polypeptides comprising two large complexes; (c) the FLA10 kinesin-II
and IFT particle polypeptides, in addition to being
found in flagella, are highly concentrated around the
flagellar basal bodies; and, (d) mutations affecting homologs of two of the IFT particle polypeptides in Caenorhabditis elegans result in defects in the sensory cilia located on the dendritic processes of sensory neurons.
In the accompanying report by Pazour, G.J., C.G. Wilkerson, and G.B. Witman (1998. J. Cell Biol. 141:979-992),
a Chlamydomonas mutant (fla14) is described in which
only the retrograde transport of IFT particles is disrupted, resulting in assembly-defective flagella filled
with an excess of IFT particles. This microtubule-
dependent transport process, IFT, defined by mutants
in both the anterograde (fla10) and retrograde (fla14)
transport of isolable particles, is probably essential for
the maintenance and assembly of all eukaryotic motile
flagella and nonmotile sensory cilia.
School of Botany,
University of Melbourne, Parkville, 3052, Victoria, Australia
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