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© The Rockefeller University Press, 0021-9525/1998//1181 $5.00
The Journal of Cell Biology, Volume 141, Number 5, , 1998 1181-1191

Articles

Localization of Mad2 to Kinetochores Depends on Microtubule Attachment, Not Tension



Jennifer C. Waters*, Rey-Huei Chen{ddagger}, Andrew W. Murray{ddagger}, and E.D. Salmon*

* Biology Department, University of North Carolina, Chapel Hill, North Carolina 27599-3280; and {ddagger} Physiology Department, University of California, San Francisco, California 94143-0444

A single unattached kinetochore can delay anaphase onset in mitotic tissue culture cells (Rieder, C.L., A. Schultz, R. Cole, G. Sluder. 1994. J. Cell Biol. 127:1301–1310). Kinetochores in vertebrate cells contain multiple binding sites, and tension is generated at kinetochores after attachment to the plus ends of spindle microtubules. Checkpoint component Mad2 localizes selectively to unattached kinetochores (Chen, R.-H., J.C. Waters, E.D. Salmon, and A.W. Murray. 1996. Science. 274:242–246; Li, Y., and R. Benezra. Science. 274: 246–248) and disappears from kinetochores by late metaphase, when chromosomes are properly attached to the spindle. Here we show that Mad2 is lost from PtK1 cell kinetochores as they accumulate microtubules and re-binds previously attached kinetochores after microtubules are depolymerized with nocodazole. We also show that when kinetochore microtubules in metaphase cells are stabilized with taxol, tension at kinetochores is lost. The phosphoepitope 3f3/2, which has been shown to become dephosphorylated in response to tension at the kinetochore (Nicklas, R.B., S.C. Ward, and G.J. Gorbsky. 1995. J. Cell Biol. 130:929–939), is phosphorylated on all 22 kinetochores after tension is reduced with taxol. In contrast, Mad2 only localized to an average of 2.6 out of the 22 kinetochores in taxol-treated PtK1 cells. Therefore, loss of tension at kinetochores occupied by microtubules is insufficient to induce Mad2 to accumulate on kinetochores, whereas unattached kinetochores consistently bind Mad2. We also found that microinjecting antibodies against Mad2 caused cells arrested with taxol to exit mitosis after ~12 min, while uninjected cells remained in mitosis for at least 6 h, demonstrating that Mad2 is necessary for maintenance of the taxol-induced mitotic arrest. We conclude that kinetochore microtubule attachment stops the Mad2 interactions at kinetochores which are important for inhibiting anaphase onset.

This work was supported, in part, by National Institutes of Health (NIH) grant GM-24364 to E.D. Salmon and grants from NIH and the Packard Foundation to A.W. Murray.

Address all correspondence to J.C. Waters, Biology Department, University of North Carolina, Chapel Hill, NC 27599-3280. Tel.: (919) 962-2354. Fax: (919) 962-1625. E-mail: jwaters{at}email.unc.edu

R.-H. Chen's present address is Biochemistry Molecular and Cell Biology Department, Cornell University, Ithaca, NY 14850.


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