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© The Rockefeller University Press, 0021-9525/1998//1217 $5.00
The Journal of Cell Biology, Volume 141, Number 5, , 1998 1217-1228

Articles

FH3, A Domain Found in Formins, Targets the Fission Yeast Formin Fus1 to the Projection Tip During Conjugation



Janni Petersen*, Olaf Nielsen*, Richard Egel*, and Iain M. Hagan{ddagger}

* Department of Genetics, Institute of Molecular Biology, ØsterFarimagsgade 2A, University of Copenhagen, DK-1353 Copenhagen K, Denmark; and {ddagger} School of Biological Sciences, University of Manchester, Manchester M13 9PT, United Kingdom

Formins are involved in diverse aspects of morphogenesis, and share two regions of homology: FH1 and FH2. We describe a new formin homology region, FH3. FH3 is an amino-terminal domain that differs from the Rho binding site identified in Bni1p and p140mDia. The Schizosaccharomyces pombe formin Fus1 is required for conjugation, and is localized to the projection tip in cells of mating pairs. We replaced genomic fus1+ with green fluorescent protein (GFP)- tagged versions that lacked either the FH1, FH2, or FH3 domain. Deletion of any FH domain essentially abolished mating. FH3, but neither FH1 nor FH2, was required for Fus1 localization. An FH3 domain–GFP fusion protein localized to the projection tips of mating pairs. Thus, the FH3 domain alone can direct protein localization. The FH3 domains of both Fus1 and the S. pombe cytokinesis formin Cdc12 were able to localize GFP to the spindle pole body in half of the late G2 cells in a vegetatively growing population. Expression of both FH3-GFP fusions also affected cytokinesis. Overexpression of the spindle pole body component Sad1 altered the distribution of both Sad1 and the FH3-GFP domain. Together these data suggest that proteins at multiple sites can interact with FH3 domains.

Abbreviations used in this paper: DAPI, 4',6-diamidino-2-phenylindole; FH, formin homology; GFP, green fluorescent protein; GST, glutathione S transferase; MSL, minimal sporulation media liquid; SPB, spindle pole body.

Address all correspondence to Janni Petersen, Department of Genetics, Institute of Molecular Biology, Østerfarimagsgade 2A, University of Copenhagen, DK-1353 Copenhagen K, Denmark. Tel: 45-35-32-21-03; Fax: 45-35-32-21-13; E-mail: jan0302{at}biobase.dk


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