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© The Rockefeller University Press, 0021-9525/1998//1301 $5.00
The Journal of Cell Biology, Volume 141, Number 6, , 1998 1301-1310

Interaction of Vault Particles with Estrogen Receptor in the MCF-7 Breast Cancer Cell

Istituto di Patologia generale ed Oncologia, Facoltà di Medicina e Chirurgia, Seconda Università degli studi di Napoli, I-80138 Naples, Italy

A 104-kD protein was coimmunoprecipitated with the estrogen receptor from the flowtrough of a phosphocellulose chromatography of MCF-7 cell nuclear extract. mAbs to this protein identified several cDNA clones coding for the human 104-kD major vault protein. Vaults are large ribonucleoprotein particles of unknown function present in all eukaryotic cells. They have a complex morphology, including several small molecules of RNA, but a single protein species, the major vault protein, accounts for >70% of their mass. Their shape is reminiscent of the nucleopore central plug, but no proteins of known function have been described to interact with them. Western blot analysis of vaults purified on sucrose gradient showed the presence of estrogen receptor co-migrating with the vault peak. The AER317 antibody to estrogen receptor coimmunoprecipitated the major vault protein and the vault RNA also in the 20,000 g supernatant fraction. Reconstitution experiments of estrogen receptor fragments with the major vault protein mapped the site of the interaction between amino acids 241 and 280 of human estrogen receptor, where the nuclear localization signal sequences are located. Estradiol treatment of cells increased the amount of major vault protein present in the nuclear extract and coimmunoprecipitated with estrogen receptor, whereas the anti-estrogen ICI182,780 had no effect. The hormone-dependent interaction of vaults with estrogen receptor was reproducible in vitro and was prevented by sodium molybdate. Antibodies to progesterone and glucocorticoid receptors were able to coimmunoprecipitate the major vault protein. The association of nuclear receptors with vaults could be related to their intracellular traffic.

Abbreviations used in this paper: aa, amino acids; GST, glutathione- S-transferase; LRP, lung resistance–related protein; hsp90, 90-kD heat shock protein; vRNA, vault RNA.

This investigation was supported by the Italian Ministry for University and Scientific and Technological Research and the Italian Association for Cancer Research (AIRC).

Address all correspondence to Bruno Moncharmont, Istituto di Patologia generale ed Oncologia, Facoltà di Medicina e Chirurgia, Seconda Università degli studi di Napoli, Larghetto Sant' Aniello a Caponapoli, 2 I-80138 Naples, Italy. Tel.: +39 81 5665686. Fax: +39 81 5665695. E-mail: mobruno @unina.it

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