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J. Cell Biol.,
Volume 141, Number 7, June 29, 1998 1601-1611
* Department of Pharmacology and Several neuronal RNAs have been identified
in dendrites, and it has been suggested that the dendritic location of these RNAs may be relevant to the
spatiotemporal regulation of mosaic postsynaptic protein repertoires through transsynaptic activity. Such
regulation would require that dendritic RNAs themselves, or at least some of them, be subject to physiological control. We have therefore examined the functional regulation of somatodendritic expression levels
of dendritic BC1 RNA in hippocampal neurons in culture. BC1 RNA, an RNA polymerase III transcript that
is a component of a ribonucleoprotein particle, became
first detectable in somatodendritic domains of developing hippocampal neurons at times of initial synapse formation. BC1 RNA was identified only in such neurons that had established synapses on cell bodies and/or developing dendritic arbors. When synaptic contact formation was initiated later in low-density cultures, BC1
expression was coordinately delayed. Inhibition of neuronal activity in hippocampal neurons resulted in a substantial but reversible reduction of somatodendritic BC1 expression. We conclude that expression of BC1
RNA in somatic and dendritic domains of hippocampal
neurons is regulated in development, and is dependent
upon neuronal activity. These results establish (for the
first time to our knowledge) that an RNA polymerase
III transcript can be subject to control through physiological activity in nerve cells.
Department of Neurology, State University of New York, Health Science Center at
Brooklyn, Brooklyn, New York 11203; § Center for Research on Occupational and Environmental Toxicology, Oregon Health
Sciences University, Portland, Oregon 97201; and Institute for Experimental Pathology, ZMBE, University of Münster,
D-48149 Münster, Germany
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