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J. Cell Biol.,
Volume 142, Number 1, July 13, 1998 191-202
* Department of Anatomy and Neurobiology, Colorado State University, Fort Collins, Colorado; During central nervous system development,
neurons differentiate distinct axonal and dendritic processes whose outgrowth is influenced by environmental
cues. Given the known intrinsic differences between axons and dendrites and that little is known about the response of dendrites to inhibitory cues, we tested the hypothesis that outgrowth of differentiating axons and
dendrites of hippocampal neurons is differentially influenced by inhibitory environmental cues. A sensitive
growth cone behavior assay was used to assess responses of differentiating axonal and dendritic growth
cones to oligodendrocytes and oligodendrocyte-
derived, myelin-associated glycoprotein (MAG). We
report that >90% of axonal growth cones collapsed after contact with oligodendrocytes. None of the encounters between differentiating, MAP-2 positive dendritic
growth cones and oligodendrocytes resulted in growth
cone collapse. The insensitivity of differentiating dendritic growth cones appears to be acquired since they
develop from minor processes whose growth cones are inhibited (nearly 70% collapse) by contact with oligodendrocytes. Recombinant MAG(rMAG)-coated
beads caused collapse of 72% of axonal growth cones
but only 29% of differentiating dendritic growth cones.
Unlike their response to contact with oligodendrocytes, few growth cones of minor processes were inhibited by
rMAG-coated beads (20% collapsed). These results reveal the capability of differentiating growth cones of
the same neuron to partition the complex molecular
terrain they navigate by generating unique responses to
particular inhibitory environmental cues.
Division of Science, Truman
State University, Kirksville, Missouri; § Department of Neurobiology and Anatomy, University of Utah School of Medicine, Salt
Lake City, Utah; Department of Biochemistry, McGill University, Montréal, Canada; ¶ Centre for Research in Neuroscience,
Montréal General Hospital Research Institute, Montréal, Canada; and ** Department dé Pathologie, Université de Montréal,
Montréal, Canada
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