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© The Rockefeller University Press, 0021-9525/1998//59 $5.00
The Journal of Cell Biology, Volume 142, Number 1, , 1998 59-67

Articles

The LDL Receptor Clustering Motif Interacts with the Clathrin Terminal Domain in a Reverse Turn Conformation



Richard G. Kibbey*, Josep Rizo{ddagger},§, Lila M. Gierasch||, and Richard G.W. Anderson*

* Department of Cell Biology and Neuroscience, {ddagger} Department of Biochemistry, and § Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, Texas, 75235; and || Department of Chemistry, University of Massachusetts, Amherst, Massachusetts 01003

Previously the hexapeptide motif FXNPXY807 in the cytoplasmic tail of the LDL receptor was shown to be essential for clustering in clathrin-coated pits. We used nuclear magnetic resonance line-broadening and transferred nuclear Overhauser effect measurements to identify the molecule in the clathrin lattice that interacts with this hexapeptide, and determined the structure of the bound motif. The wild-type peptide bound in a single conformation with a reverse turn at residues NPVY. Tyr807Ser, a peptide that harbors a mutation that disrupts receptor clustering, displayed markedly reduced interactions. Clustering motif peptides interacted with clathrin cages assembled in the presence or absence of AP2, with recombinant clathrin terminal domains, but not with clathrin hubs. The identification of terminal domains as the primary site of interaction for FXNPXY807 suggests that adaptor molecules are not required for receptor-mediated endocytosis of LDL, and that at least two different tyrosine-based internalization motifs exist for clustering receptors in coated pits.

Key Words: clathrin • endocytosis • LDL receptor • nuclear magnetic resonance

Abbreviations used in this paper: GST, glutathione-S-transferase; m, medium; NMR, nuclear magnetic resonance; NOE, nuclear Overhauser effect; PTB, phosphotyrosine binding; s, strong; w, weak.

Address all correspondence to Richard G.W. Anderson, Department of Cell Biology and Neuroscience, University of Texas Southwestern Medical Center, Dallas, TX 75235-9039. Tel.: 214-648-2346; Fax: 214-648-7577; E-mail: anders06{at}utsw.swmed.edu


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