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© The Rockefeller University Press, 0021-9525/1998//421 $5.00
The Journal of Cell Biology, Volume 142, Number 2, , 1998 421-434

The Cytosolic DnaJ-like Protein Djp1p Is Involved Specifically in Peroxisomal Protein Import

Department of Biochemistry, Academic Medical Center, 1105 AZ Amsterdam, The Netherlands

The Saccharomyces cerevisiae DJP1 gene encodes a cytosolic protein homologous to Escherichia coli DnaJ. DnaJ homologues act in conjunction with molecular chaperones of the Hsp70 protein family in a variety of cellular processes. Cells with a DJP1 gene deletion are viable and exhibit a novel phenotype among cytosolic J-protein mutants in that they have a specific impairment of only one organelle, the peroxisome. The phenotype was also unique among peroxisome assembly mutants: peroxisomal matrix proteins were mislocalized to the cytoplasm to a varying extent, and peroxisomal structures failed to grow to full size and exhibited a broad range of buoyant densities. Import of marker proteins for the endoplasmic reticulum, nucleus, and mitochondria was normal. Furthermore, the metabolic adaptation to a change in carbon source, a complex multistep process, was unaffected in a DJP1 gene deletion mutant. We conclude that Djp1p is specifically required for peroxisomal protein import.

Key Words: molecular chaperone • peroxisome • DnaJ • import • Saccharomyces cerevisiae

Abbreviations used in this paper: CTA, catalase A; Djp1p, DnaJ-like protein required for peroxisomal protein import; Gal-1-PUT, galactose-1-phosphate uridylyltransferase; GFP, green fluorescent protein; Hsp, heat-shock protein; Mdh, malate dehydrogenase; NH₂-tag, NH₂-terminal hemagglutinin epitope tag; NLS, nuclear localization signal; ORF, open reading frame; pex, peroxin; PGI, phosphoglucose isomerase; PTS, peroxisome targeting signal.

Address all correspondence to I. Braakman, Department of Biochemistry, Academic Medical Center, 1105 AZ Amsterdam, The Netherlands. Tel.: +31-20-566-3297. Fax +31-20-691-5519. E-mail: i.braakman{at}amc.uva.nl

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