J. Cell Biol., Volume 142, Number 2, July 27, 1998 421-434

The Cytosolic DnaJ-like Protein Djp1p Is Involved Specifically in Peroxisomal Protein Import

Ewald H. Hettema, Caroline C.M. Ruigrok, Marian Groot Koerkamp, Marlene van den Berg, Henk F. Tabak, Ben Distel, and Ineke Braakman

Department of Biochemistry, Academic Medical Center, 1105 AZ Amsterdam, The Netherlands

The Saccharomyces cerevisiae DJP1 gene encodes a cytosolic protein homologous to Escherichia coli DnaJ. DnaJ homologues act in conjunction with molecular chaperones of the Hsp70 protein family in a variety of cellular processes. Cells with a DJP1 gene deletion are viable and exhibit a novel phenotype among cytosolic J-protein mutants in that they have a specific impairment of only one organelle, the peroxisome. The phenotype was also unique among peroxisome assembly mutants: peroxisomal matrix proteins were mislocalized to the cytoplasm to a varying extent, and peroxisomal structures failed to grow to full size and exhibited a broad range of buoyant densities. Import of marker proteins for the endoplasmic reticulum, nucleus, and mitochondria was normal. Furthermore, the metabolic adaptation to a change in carbon source, a complex multistep process, was unaffected in a DJP1 gene deletion mutant. We conclude that Djp1p is specifically required for peroxisomal protein import.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

• Zolman, B. K., Monroe-Augustus, M., Silva, I. D., Bartel, B. (2005). Identification and Functional Characterization of Arabidopsis PEROXIN4 and the Interacting Protein PEROXIN22. Plant Cell 17: 3422-3435 [Abstract] [Full Text]  
• Kragt, A., Voorn-Brouwer, T., van den Berg, M., Distel, B. (2005). Endoplasmic Reticulum-directed Pex3p Routes to Peroxisomes and Restores Peroxisome Formation in a Saccharomyces cerevisiae pex3{Delta} Strain. J. Biol. Chem. 280: 34350-34357 [Abstract] [Full Text]  
• Lu, J. M.-Y., Deschenes, R. J., Fassler, J. S. (2004). Role for the Ran Binding Protein, Mog1p, in Saccharomyces cerevisiae SLN1-SKN7 Signal Transduction. Eukaryot Cell 3: 1544-1556 [Abstract] [Full Text]  
• Borkovich, K. A., Alex, L. A., Yarden, O., Freitag, M., Turner, G. E., Read, N. D., Seiler, S., Bell-Pedersen, D., Paietta, J., Plesofsky, N., Plamann, M., Goodrich-Tanrikulu, M., Schulte, U., Mannhaupt, G., Nargang, F. E., Radford, A., Selitrennikoff, C., Galagan, J. E., Dunlap, J. C., Loros, J. J., Catcheside, D., Inoue, H., Aramayo, R., Polymenis, M., Selker, E. U., Sachs, M. S., Marzluf, G. A., Paulsen, I., Davis, R., Ebbole, D. J., Zelter, A., Kalkman, E. R., O'Rourke, R., Bowring, F., Yeadon, J., Ishii, C., Suzuki, K., Sakai, W., Pratt, R. (2004). Lessons from the Genome Sequence of Neurospora crassa: Tracing the Path from Genomic Blueprint to Multicellular Organism. Microbiol. Mol. Biol. Rev. 68: 1-108 [Abstract] [Full Text]  
• Klein, A. T. J., van den Berg, M., Bottger, G., Tabak, H. F., Distel, B. (2002). Saccharomyces cerevisiae Acyl-CoA Oxidase Follows a Novel, Non-PTS1, Import Pathway into Peroxisomes That Is Dependent on Pex5p. J. Biol. Chem. 277: 25011-25019 [Abstract] [Full Text]  
• Fransen, M., Brees, C., Ghys, K., Amery, L., Mannaerts, G. P., Ladant, D., Van Veldhoven, P. P. (2002). Analysis of Mammalian Peroxin Interactions Using a Non-transcription-based Bacterial Two-hybrid Assay. Mol. Cell. Proteomics 1: 243-252 [Abstract] [Full Text]  
• Hoepfner, D., van den Berg, M., Philippsen, P., Tabak, H. F., Hettema, E. H. (2001). A role for Vps1p, actin, and the Myo2p motor in peroxisome abundance and inheritance in Saccharomyces cerevisiae. JCB 155: 979-990 [Abstract] [Full Text]  
• Bottger, G., Barnett, P., Klein, A. T. J., Kragt, A., Tabak, H. F., Distel, B. (2000). Saccharomyces cerevisiae PTS1 Receptor Pex5p Interacts with the SH3 Domain of the Peroxisomal Membrane Protein Pex13p in an Unconventional, Non-PXXP-related Manner. Mol. Biol. Cell 11: 3963-3976 [Abstract] [Full Text]  
• McClellan, A. J., Brodsky, J. L. (2000). Mutation of the ATP-Binding Pocket of SSA1 Indicates That a Functional Interaction Between Ssa1p and Ydj1p Is Required for Post-translational Translocation Into the Yeast Endoplasmic Reticulum. Genetics 156: 501-512 [Abstract] [Full Text]  
• Kuhn, A. N., Brow, D. A. (2000). Suppressors of a Cold-Sensitive Mutation in Yeast U4 RNA Define Five Domains in the Splicing Factor Prp8 That Influence Spliceosome Activation. Genetics 155: 1667-1682 [Abstract] [Full Text]  
• Yan, W., Craig, E. A. (1999). The Glycine-Phenylalanine-Rich Region Determines the Specificity of the Yeast Hsp40 Sis1. Mol. Cell. Biol. 19: 7751-7758 [Abstract] [Full Text]  
• Mullen, R. T., Lisenbee, C. S., Miernyk, J. A., Trelease, R. N. (1999). Peroxisomal Membrane Ascorbate Peroxidase Is Sorted to a Membranous Network That Resembles a Subdomain of the Endoplasmic Reticulum. Plant Cell 11: 2167-2186 [Abstract] [Full Text]  
• Koller, A., Snyder, W. B., Faber, K. N., Wenzel, T. J., Rangell, L., Keller, G. A., Subramani, S. (1999). Pex22p of Pichia pastoris, Essential for Peroxisomal Matrix Protein Import, Anchors the Ubiquitin-conjugating Enzyme, Pex4p, on the Peroxisomal Membrane. JCB 146: 99-112 [Abstract] [Full Text]  

Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents