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© The Rockefeller University Press, 0021-9525/1998//435 $5.00
The Journal of Cell Biology, Volume 142, Number 2, , 1998 435-442

Articles

Translation of the Chloroplast psbA mRNA Requires the Nuclear-encoded Poly(A)-binding Protein, RB47



Christopher B. Yohn*, Amybeth Cohen{ddagger}, Cristen Rosch§, Michael R. Kuchka§, and Stephen P. Mayfield*

* Department of Cell Biology, and The Skaggs Institute of Chemical Biology, The Scripps Research Institute, La Jolla, California 92037; {ddagger} Department of Biological Science, The California State University, Fullerton, California 92834-6850; and § Department of Biological Science, Lehigh University, Bethlehem, Pennsylvania 18105

A set of nuclear mutants of C. reinhardtii were identified that specifically lack translation of the chloroplast-encoded psbA mRNA, which encodes the photosystem II reaction center polypeptide D1. Two of these mutants are deficient in the 47-kD member (RB47) of the psbA RNA-binding complex, which has previously been identified both genetically and biochemically as a putative translational activator of the chloroplast psbA mRNA. RB47 is a member of the poly(A)-binding protein family, and binds with high affinity and specificity to the 5' untranslated region of the psbA mRNA. The results presented here confirm RB47's role as a message-specific translational activator in the chloroplast, and bring together genetic and biochemical data to form a cohesive model for light- activated translational regulation in the chloroplast.

Key Words: genetic translation • initiation factors • translational regulation • gene expression

Abbreviations used in this paper: FD, ferrodoxin; hf, high fluorescence; LHC, light-harvesting complex; OEE1, oxygen evolution complex; PABP, poly(A)-binding protein; PDI, protein disulfide isomerase; PS II, photosystem II; T1-GMS, T1 RNase protection-gel mobility shift; TAP, Tris-acetate-phosphate; UTR, untranslated region; wt, wild type.

Address all correspondence to Stephen P. Mayfield, Department of Cell Biology, and The Skaggs Institute of Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037. Tel.: (619) 784-9848. Fax: (619) 784-9840. E-mail: mayfield{at}scripps.edu


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