Regulation of Cell Polarity by Microtubules in Fission Yeast

doi:10.1083/jcb.142.2.457

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© The Rockefeller University Press, 0021-9525/1998//457 $5.00
The Journal of Cell Biology, Volume 142, Number 2, , 1998 457-471

Articles

Regulation of Cell Polarity by Microtubules in Fission Yeast

Kenneth E. Sawin and Paul Nurse

Imperial Cancer Research Fund, London WC2A 3PX, United Kingdom

To investigate the role of microtubules in regulating cellpolarity in Schizosaccharomyces pombe, we have developed asystem in which normally cylindrical fission yeast synchronouslyform branched cells at high frequency upon treatment with themicrotubule-depolymerizing drug thiabendazole (TBZ). Branching depends on both elevated temperature and cell cycle stateand occurs at high frequency only when TBZ is added to cellsthat have not yet passed through New-End Take-Off (NETO), thenormal transition from monopolar to bipolar growth. This suggeststhat microtubules may be of greatest physiological importancefor the maintenance of cell shape at specific points in the cell cycle. The localization of three different proteins normallyfound at cell ends—cortical F-actin, tea1, and an ral3(scd2)–green fluorescent protein (GFP) fusion—isdisrupted by TBZ treatment. However, these proteins can eventuallyreturn to cell ends in the absence of microtubules, indicatingthat although their localization to ends normally depends onmicrotubules, they may recover by alternative mechanisms. Inaddition, TBZ induces a shift in ral3–GFP distributionfrom cell ends to the cell middle, suggesting that a protein complex containing ral3 may be part of the cue that specifiesthe position of branch formation.

Key Words: S. pombe • microtubules • thiabendazole • actin • cell polarity

Abbreviations used in this paper: GFP, green fluorescent protein; HU, hydroxyurea; LatA, latrunculin A; NETO, New-End Take-Off; TBZ, thiabendazole.

Address all correspondence to K.E. Sawin, Imperial Cancer Research Fund, 44 Lincoln's Inn Fields, London WC2A 3PX, UK. Tel.: 44-171-269-3276.Fax: 44-171-269-3610. E-mail: sawin{at}europa.lif.icnet.uk

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