Regulation of Cell Polarity by Microtubules in Fission Yeast

doi:10.1083/jcb.142.2.457

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J. Cell Biol., Volume 142, Number 2, July 27, 1998 457-471

Regulation of Cell Polarity by Microtubules in Fission Yeast

Kenneth E. Sawin, and Paul Nurse

Imperial Cancer Research Fund, London WC2A 3PX, United Kingdom

To investigate the role of microtubules in regulating cell polarity in Schizosaccharomyces pombe, we have developed a systemin which normally cylindrical fission yeast synchronously formbranched cells at high frequency upon treatment with the microtubule-depolymerizingdrug thiabendazole (TBZ). Branching depends on both elevated temperatureand cell cycle state and occurs at high frequency only when TBZis added to cells that have not yet passed through New-End Take-Off(NETO), the normal transition from monopolar to bipolar growth.This suggests that microtubules may be of greatest physiologicalimportance for the maintenance of cell shape at specific pointsin the cell cycle. The localization of three different proteinsnormally found at cell ends $---$ cortical F-actin, tea1, and an ral3(scd2)-green fluorescent protein (GFP) fusion $---$ is disrupted byTBZ treatment. However, these proteins can eventually return tocell ends in the absence of microtubules, indicating that althoughtheir localization to ends normally depends on microtubules, theymay recover by alternative mechanisms. In addition, TBZ inducesa shift in ral3-GFP distribution from cell ends to the cell middle,suggesting that a protein complex containing ral3 may be partof the cue that specifies the position of branch formation.

Key words: S. pombe; microtubules; thiabendazole; actin; cell polarity

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