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J. Cell Biol.,
Volume 142, Number 2, July 27, 1998 537-544
* Myogenesis Research Laboratory, Massachusetts General Hospital, Charlestown, Massachusetts 02129; and We show that Bcl-2 expression in skeletal
muscle cells identifies an early stage of the myogenic
pathway, inhibits apoptosis, and promotes clonal expansion. Bcl-2 expression was limited to a small proportion of the mononucleate cells in muscle cell cultures, ranging from ~1-4% of neonatal and adult mouse muscle cells to ~5-15% of the cells from the C2C12 muscle
cell line. In rapidly growing cultures, some of the Bcl-2-positive cells coexpressed markers of early stages of
myogenesis, including desmin, MyoD, and Myf-5. In
contrast, Bcl-2 was not expressed in multinucleate myotubes or in those mononucleate myoblasts that expressed markers of middle or late stages of myogenesis,
such as myogenin, muscle regulatory factor 4 (MRF4),
and myosin. The small subset of Bcl-2-positive C2C12
cells appeared to resist staurosporine-induced apoptosis. Furthermore, though myogenic cells from genetically Bcl-2-null mice formed myotubes normally, the
muscle colonies produced by cloned Bcl-2-null cells
contained only about half as many cells as the colonies
produced by cells from wild-type mice. This result suggests that, during clonal expansion from a muscle progenitor cell, the number of progeny obtained is greater
when Bcl-2 is expressed.
Program in
Neuroscience, Harvard Medical School, Boston, Massachusetts 02115
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