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© The Rockefeller University Press, 0021-9525/1998//613 $5.00
The Journal of Cell Biology, Volume 142, Number 3, , 1998 613-623

Regular Articles

The Sorting of Mitochondrial DNA and Mitochondrial Proteins in Zygotes: Preferential Transmission of Mitochondrial DNA to the Medial Bud



Koji Okamoto, Philip S. Perlman, and Ronald A. Butow

Department of Molecular Biology and Oncology, University of Texas Southwestern Medical Center, Dallas, Texas 75235-9148

Green fluorescent protein (GFP) was used to tag proteins of the mitochondrial matrix, inner, and outer membranes to examine their sorting patterns relative to mtDNA in zygotes of synchronously mated yeast cells in {rho}+ x {rho}0 crosses. When transiently expressed in one of the haploid parents, each of the marker proteins distributes throughout the fused mitochondrial reticulum of the zygote before equilibration of mtDNA, although the membrane markers equilibrate slower than the matrix marker. A GFP-tagged form of Abf2p, a mtDNA binding protein required for faithful transmission of {rho}+ mtDNA in vegetatively growing cells, colocalizes with mtDNA in situ. In zygotes of a {rho}+ x {rho}+ cross, in which there is little mixing of parental mtDNAs, Abf2p–GFP prelabeled in one parent rapidly equilibrates to most or all of the mtDNA, showing that the mtDNA compartment is accessible to exchange of proteins. In {rho}+ x {rho}0 crosses, mtDNA is preferentially transmitted to the medial diploid bud, whereas mitochondrial GFP marker proteins distribute throughout the zygote and the bud. In zygotes lacking Abf2p, mtDNA sorting is delayed and preferential sorting is reduced. These findings argue for the existence of a segregation apparatus that directs mtDNA to the emerging bud.

Key Words: yeast • mitochondria • mitochondrial DNA • zygotes • DNA segregation

Abbreviations used in this paper: CS1, citrate synthase 1; DAPI, 4',6-diamino-2-phenylindole; GFP, green fluorescent protein; HMG, high mobility group; Tom, translocase outer membrane.

Address all correspondence to Ronald A. Butow, Department of Molecular Biology and Oncology, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd., Dallas, TX 75235-9148. Tel.: (214) 648-1465. Fax: (214) 648-1488. E-mail: butow{at}swmed.edu


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