Growth Factor-induced p42/p44 MAPK Nuclear Translocation and Retention Requires Both MAPK Activation and Neosynthesis of Nuclear Anchoring Proteins

doi:10.1083/jcb.142.3.625

This Article

	Full Text
	Full Text (PDF, 538K)
	PPT slides of all figures
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JCB
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Lenormand, P.
	Articles by Pouysségur, J.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Lenormand, P.
	Articles by Pouysségur, J.


Social Bookmarking

	What's this?

© The Rockefeller University Press, 0021-9525/1998//625 $5.00
The Journal of Cell Biology, Volume 142, Number 3, , 1998 625-633

Regular Articles

Growth Factor–induced p42/p44 MAPK Nuclear Translocation and Retention Requires Both MAPK Activation and Neosynthesis of Nuclear Anchoring Proteins

Philippe Lenormand, Jean-Marc Brondello, Anne Brunet, and Jacques Pouysségur

Centre de Biochimie–Centre National de la Recherche Scientifique (CNRS) UMR 6543, Université de Nice, 06108 Nice, France

Mitogen-activated protein kinases (p42/p44 MAPK, also calledErk2 and Erk1) are key mediators of signal transduction fromthe cell surface to the nucleus. We have previously shown thatthe activation of p42/p44 MAPK required for transduction ofmitogenic signaling is associated with a rapid nuclear translocationof these kinases. However, the means by which p42 and p44 MAPKtranslocate into the nucleus after cytoplasmic activation isstill not understood and cannot simply be deduced from theirprotein sequences. In this study, we have demonstrated thatactivation of the p42/ p44 MAPK pathway was necessary and sufficientfor triggering nuclear translocation of p42 and p44 MAPK. First, addition of the MEK inhibitor PD 98059, which blocksactivation of the p42/p44 MAPK pathway, impedes the nuclearaccumulation, whereas direct activation of the p42/p44 MAPKpathway by the chimera ${Delta}$ Raf-1:ER is sufficient to promote nuclearaccumulation of p42/p44 MAPK. In addition, we have shown thatthis nuclear accumulation of p42/p44 MAPK required the neosynthesisof short-lived proteins. Indeed, inhibitors of protein synthesisabrogate nuclear accumulation in response to serum and acceleratep42/p44 MAPK nuclear efflux under conditions of persistent p42/p44 MAPK activation. In contrast, inhibition of targetedproteolysis by the proteasome synergistically potentiated p42/p44MAPK nuclear localization by nonmitogenic agonists and markedlyprolonged nuclear localization of p42/p44 MAPK after mitogenicstimulation. We therefore conclude that the MAPK nuclear translocationrequires both activation of the p42/p44 MAPK module and neosynthesisof short-lived proteins that we postulate to be nuclear anchors.

Key Words: growth factors • signal transduction • protein kinases • cell compartmentation • cell nucleus

Abbreviations used in this paper: CHX, cycloheximide; ERK, extra cellular regulated kinase; JNK, Jun NH₂-terminal Kinase; LLnL, N-Acetyl-Leu-Leu-norLeucinal; MAPK, mitogen-activated protein kinase; MEK, MAPK or Erk kinase; NLS, nuclear localization signal; PD 98059, Park Davis 98059; TPA, 12-O-tetra decanoyl phorbol myristate acetate.

Address all correspondence to Philippe Lenormand, Centre deBiochimie–CNRS UMR 6543, Université de Nice, 06108Nice, France. Tel.: (33) 492 07 64 27. Fax: (33) 492 07 6432. E-mail: lenorman{at}unice.fr

CiteULike

Complore

Connotea

Del.icio.us Add to Digg

Digg

Facebook

Technorati

Twitter What's this?

This article has been cited by other articles:

Armstrong, S. P., Caunt, C. J., McArdle, C. A. (2009). Gonadotropin-Releasing Hormone and Protein Kinase C Signaling to ERK: Spatiotemporal Regulation of ERK by Docking Domains and Dual-Specificity Phosphatases. Mol. Endocrinol. 23: 510-519 [Abstract] [Full Text]
Yan, Y., Black, C. P., Cao, P. T., Haferbier, J. L., Kolb, R. H., Spieker, R. S., Ristow, A. M., Cowan, K. H. (2008). {gamma}-Irradiation-Induced DNA Damage Checkpoint Activation Involves Feedback Regulation between Extracellular Signal-Regulated Kinase 1/2 and BRCA1. Cancer Res. 68: 5113-5121 [Abstract] [Full Text]
Chuderland, D., Marmor, G., Shainskaya, A., Seger, R. (2008). Calcium-mediated Interactions Regulate the Subcellular Localization of Extracellular Signal-regulated Kinases. J. Biol. Chem. 283: 11176-11188 [Abstract] [Full Text]
Dawson, C. W., Laverick, L., Morris, M. A., Tramoutanis, G., Young, L. S. (2008). Epstein-Barr Virus-Encoded LMP1 Regulates Epithelial Cell Motility and Invasion via the ERK-MAPK Pathway. J. Virol. 82: 3654-3664 [Abstract] [Full Text]
Caunt, C. J., Rivers, C. A., Conway-Campbell, B. L., Norman, M. R., McArdle, C. A. (2008). Epidermal Growth Factor Receptor and Protein Kinase C Signaling to ERK2: SPATIOTEMPORAL REGULATION OF ERK2 BY DUAL SPECIFICITY PHOSPHATASES. J. Biol. Chem. 283: 6241-6252 [Abstract] [Full Text]
Skarpen, E., Flinder, L. I., Rosseland, C. M., Orstavik, S., Wierod, L., Oksvold, M. P., Skalhegg, B. S., Huitfeldt, H. S. (2008). MEK1 and MEK2 regulate distinct functions by sorting ERK2 to different intracellular compartments. FASEB J. 22: 466-476 [Abstract] [Full Text]
Pan, H., Luo, C., Li, R., Qiao, A., Zhang, L., Mines, M., Nyanda, A. M., Zhang, J., Fan, G.-H. (2008). Cyclophilin A Is Required for CXCR4-mediated Nuclear Export of Heterogeneous Nuclear Ribonucleoprotein A2, Activation and Nuclear Translocation of ERK1/2, and Chemotactic Cell Migration. J. Biol. Chem. 283: 623-637 [Abstract] [Full Text]
Yazicioglu, M. N., Goad, D. L., Ranganathan, A., Whitehurst, A. W., Goldsmith, E. J., Cobb, M. H. (2007). Mutations in ERK2 Binding Sites Affect Nuclear Entry. J. Biol. Chem. 282: 28759-28767 [Abstract] [Full Text]
Seo, M., Lee, M.-J., Heo, J. H., Lee, Y.-I., Kim, Y., Kim, S.-Y., Lee, E.-S., Juhnn, Y.-S. (2007). G Protein beta{gamma} Subunits Augment UVB-induced Apoptosis by Stimulating the Release of Soluble Heparin-binding Epidermal Growth Factor from Human Keratinocytes. J. Biol. Chem. 282: 24720-24730 [Abstract] [Full Text]
Jang, S.-I., Lee, E.-J., Hart, P. S., Ramaswami, M., Pallos, D., Hart, T. C. (2007). Germ Line Gain of Function with SOS1 Mutation in Hereditary Gingival Fibromatosis. J. Biol. Chem. 282: 20245-20255 [Abstract] [Full Text]
Tsuji, T., Sheehy, N., Gautier, V. W., Hayakawa, H., Sawa, H., Hall, W. W. (2007). The Nuclear Import of the Human T Lymphotropic Virus Type I (HTLV-1) Tax Protein Is Carrier- and Energy-independent. J. Biol. Chem. 282: 13875-13883 [Abstract] [Full Text]
Tresini, M., Lorenzini, A., Torres, C., Cristofalo, V. J. (2007). Modulation of Replicative Senescence of Diploid Human Cells by Nuclear ERK Signaling. J. Biol. Chem. 282: 4136-4151 [Abstract] [Full Text]
Costa, M., Marchi, M., Cardarelli, F., Roy, A., Beltram, F., Maffei, L., Ratto, G. M. (2006). Dynamic regulation of ERK2 nuclear translocation and mobility in living cells. J. Cell Sci. 119: 4952-4963 [Abstract] [Full Text]
Woods, D. C., Johnson, A. L. (2006). Phosphatase Activation by Epidermal Growth Factor Family Ligands Regulates Extracellular Regulated Kinase Signaling in Undifferentiated Hen Granulosa Cells. Endocrinology 147: 4931-4940 [Abstract] [Full Text]
Ranganathan, A., Yazicioglu, M. N., Cobb, M. H. (2006). The Nuclear Localization of ERK2 Occurs by Mechanisms Both Independent of and Dependent on Energy. J. Biol. Chem. 281: 15645-15652 [Abstract] [Full Text]
Huang, T.-H., Morrison, S. L. (2006). A Trimeric Anti-HER2/neu ScFv and Tumor Necrosis Factor-{alpha} Fusion Protein Induces HER2/neu Signaling and Facilitates Repair of Injured Epithelia. J. Pharmacol. Exp. Ther. 316: 983-991 [Abstract] [Full Text]
Sigoillot, F. D., Kotsis, D. H., Serre, V., Sigoillot, S. M., Evans, D. R., Guy, H. I. (2005). Nuclear Localization and Mitogen-activated Protein Kinase Phosphorylation of the Multifunctional Protein CAD. J. Biol. Chem. 280: 25611-25620 [Abstract] [Full Text]
Mandl, M., Slack, D. N., Keyse, S. M. (2005). Specific Inactivation and Nuclear Anchoring of Extracellular Signal-Regulated Kinase 2 by the Inducible Dual-Specificity Protein Phosphatase DUSP5. Mol. Cell. Biol. 25: 1830-1845 [Abstract] [Full Text]
Peiretti, F., Canault, M., Bernot, D., Bonardo, B., Deprez-Beauclair, P., Juhan-Vague, I., Nalbone, G. (2005). Proteasome inhibition activates the transport and the ectodomain shedding of TNF-{alpha} receptors in human endothelial cells. J. Cell Sci. 118: 1061-1070 [Abstract] [Full Text]
Chen, J.-R., Plotkin, L. I., Aguirre, J. I., Han, L., Jilka, R. L., Kousteni, S., Bellido, T., Manolagas, S. C. (2005). Transient Versus Sustained Phosphorylation and Nuclear Accumulation of ERKs Underlie Anti-Versus Pro-apoptotic Effects of Estrogens. J. Biol. Chem. 280: 4632-4638 [Abstract] [Full Text]
Burack, W. R., Shaw, A. S. (2005). Live Cell Imaging of ERK and MEK: SIMPLE BINDING EQUILIBRIUM EXPLAINS THE REGULATED NUCLEOCYTOPLASMIC DISTRIBUTION OF ERK. J. Biol. Chem. 280: 3832-3837 [Abstract] [Full Text]
Gaumont-Leclerc, M.-F., Mukhopadhyay, U. K., Goumard, S., Ferbeyre, G. (2004). PEA-15 Is Inhibited by Adenovirus E1A and Plays a Role in ERK Nuclear Export and Ras-induced Senescence. J. Biol. Chem. 279: 46802-46809 [Abstract] [Full Text]
Torii, S., Nakayama, K., Yamamoto, T., Nishida, E. (2004). Regulatory Mechanisms and Function of ERK MAP Kinases. J Biochem 136: 557-561 [Abstract] [Full Text]
Karlsson, M., Mathers, J., Dickinson, R. J., Mandl, M., Keyse, S. M. (2004). Both Nuclear-Cytoplasmic Shuttling of the Dual Specificity Phosphatase MKP-3 and Its Ability to Anchor MAP Kinase in the Cytoplasm Are Mediated by a Conserved Nuclear Export Signal. J. Biol. Chem. 279: 41882-41891 [Abstract] [Full Text]
Qiu, Z., Hyman, B. T., Rebeck, G. W. (2004). Apolipoprotein E Receptors Mediate Neurite Outgrowth through Activation of p44/42 Mitogen-activated Protein Kinase in Primary Neurons. J. Biol. Chem. 279: 34948-34956 [Abstract] [Full Text]
Lee, J., Rudd, J. J., Macioszek, V. K., Scheel, D. (2004). Dynamic Changes in the Localization of MAPK Cascade Components Controlling Pathogenesis-related (PR) Gene Expression during Innate Immunity in Parsley. J. Biol. Chem. 279: 22440-22448 [Abstract] [Full Text]
Chen, L., Carter-Su, C. (2004). Adapter Protein SH2-B{beta} Undergoes Nucleocytoplasmic Shuttling: Implications for Nerve Growth Factor Induction of Neuronal Differentiation. Mol. Cell. Biol. 24: 3633-3647 [Abstract] [Full Text]
Raviv, Z., Kalie, E., Seger, R. (2004). MEK5 and ERK5 are localized in the nuclei of resting as well as stimulated cells, while MEKK2 translocates from the cytosol to the nucleus upon stimulation. J. Cell Sci. 117: 1773-1784 [Abstract] [Full Text]
Whitehurst, A. W., Robinson, F. L., Moore, M. S., Cobb, M. H. (2004). The Death Effector Domain Protein PEA-15 Prevents Nuclear Entry of ERK2 by Inhibiting Required Interactions. J. Biol. Chem. 279: 12840-12847 [Abstract] [Full Text]
Pratt, P. F., Bokemeyer, D., Foschi, M., Sorokin, A., Dunn, M. J. (2003). Alterations in Subcellular Localization of p38 MAPK Potentiates Endothelin-stimulated COX-2 Expression in Glomerular Mesangial Cells. J. Biol. Chem. 278: 51928-51936 [Abstract] [Full Text]
Julien, C., Coulombe, P., Meloche, S. (2003). Nuclear Export of ERK3 by a CRM1-dependent Mechanism Regulates Its Inhibitory Action on Cell Cycle Progression. J. Biol. Chem. 278: 42615-42624 [Abstract] [Full Text]
Snaar-Jagalska, B. E., Krens, S. F. G., Robina, I., Wang, L.-X., Spaink, H. P. (2003). Specific activation of ERK pathways by chitin oligosaccharides in embryonic zebrafish cell lines. Glycobiology 13: 725-732 [Abstract] [Full Text]
Nilsen, J., Brinton, R. D. (2003). Divergent impact of progesterone and medroxyprogesterone acetate (Provera) on nuclear mitogen-activated protein kinase signaling. Proc. Natl. Acad. Sci. USA 100: 10506-10511 [Abstract] [Full Text]
Blackhall, F. H., Pintilie, M., Michael, M., Leighl, N., Feld, R., Tsao, M.-S., Shepherd, F. A. (2003). Expression and Prognostic Significance of Kit, Protein Kinase B, and Mitogen-activated Protein Kinase in Patients with Small Cell Lung Cancer. Clin. Cancer Res. 9: 2241-2247 [Abstract] [Full Text]
Guha, S., Lunn, J. A., Santiskulvong, C., Rozengurt, E. (2003). Neurotensin Stimulates Protein Kinase C-dependent Mitogenic Signaling in Human Pancreatic Carcinoma Cell Line PANC-1. Cancer Res. 63: 2379-2387 [Abstract] [Full Text]
Zhao, L., Brinton, R. D. (2003). Vasopressin-Induced Cytoplasmic and Nuclear Calcium Signaling in Embryonic Cortical Astrocytes: Dynamics of Calcium and Calcium-Dependent Kinase Translocation. J. Neurosci. 23: 4228-4239 [Abstract] [Full Text]
Maiyar, A. C., Leong, M. L.L., Firestone, G. L. (2003). Importin-alpha Mediates the Regulated Nuclear Targeting of Serum- and Glucocorticoid-inducible Protein Kinase (Sgk) by Recognition of a Nuclear Localization Signal in the Kinase Central Domain. Mol. Biol. Cell 14: 1221-1239 [Abstract] [Full Text]
Blackwell, E., Halatek, I. M., Kim, H.-J. N., Ellicott, A. T., Obukhov, A. A., Stone, D. E. (2003). Effect of the Pheromone-Responsive G{alpha} and Phosphatase Proteins of Saccharomyces cerevisiae on the Subcellular Localization of the Fus3 Mitogen-Activated Protein Kinase. Mol. Cell. Biol. 23: 1135-1150 [Abstract] [Full Text]
Thimmaiah, K. N., Easton, J., Huang, S., Veverka, K. A., Germain, G. S., Harwood, F. C., Houghton, P. J. (2003). Insulin-like Growth Factor I-mediated Protection from Rapamycin-induced Apoptosis Is Independent of Ras-Erk1-Erk2 and Phosphatidylinositol 3'-Kinase-Akt Signaling Pathways. Cancer Res. 63: 364-374 [Abstract] [Full Text]
Bjorndal, B., Helleland, C., Boe, S.-O., Gudbrandsen, O. A., Kalland, K.-H., Bohov, P., Berge, R. K., Lillehaug, J. R. (2002). Nuclear import of factors involved in signaling is inhibited in C3H/10T1/2 cells treated with tetradecylthioacetic acid. J. Lipid Res. 43: 1630-1640 [Abstract] [Full Text]
Shibayama, S., Shibata-Seita, R., Miura, K., Kirino, Y., Takishima, K. (2002). Identification of a C-terminal Region That Is Required for the Nuclear Translocation of ERK2 by Passive Diffusion. J. Biol. Chem. 277: 37777-37782 [Abstract] [Full Text]
Orlowski, R. Z., Small, G. W., Shi, Y. Y. (2002). Evidence That Inhibition of p44/42 Mitogen-activated Protein Kinase Signaling Is a Factor in Proteasome Inhibitor-mediated Apoptosis. J. Biol. Chem. 277: 27864-27871 [Abstract] [Full Text]
Tanimura, S., Nomura, K., Ozaki, K.-i., Tsujimoto, M., Kondo, T., Kohno, M. (2002). Prolonged Nuclear Retention of Activated Extracellular Signal-regulated Kinase 1/2 Is Required for Hepatocyte Growth Factor-induced Cell Motility. J. Biol. Chem. 277: 28256-28264 [Abstract] [Full Text]
Whitehurst, A. W., Wilsbacher, J. L., You, Y., Luby-Phelps, K., Moore, M. S., Cobb, M. H. (2002). ERK2 enters the nucleus by a carrier-independent mechanism. Proc. Natl. Acad. Sci. USA 99: 7496-7501 [Abstract] [Full Text]
Xu, L., Fukumura, D., Jain, R. K. (2002). Acidic Extracellular pH Induces Vascular Endothelial Growth Factor (VEGF) in Human Glioblastoma Cells via ERK1/2 MAPK Signaling Pathway. MECHANISM OF LOW pH-INDUCED VEGF. J. Biol. Chem. 277: 11368-11374 [Abstract] [Full Text]
Robinson, M. J., Xu, B.-e, Stippec, S., Cobb, M. H. (2002). Different Domains of the Mitogen-activated Protein Kinases ERK3 and ERK2 Direct Subcellular Localization and Upstream Specificity in Vivo. J. Biol. Chem. 277: 5094-5100 [Abstract] [Full Text]
Stanciu, M., DeFranco, D. B. (2002). Prolonged Nuclear Retention of Activated Extracellular Signal-regulated Protein Kinase Promotes Cell Death Generated by Oxidative Toxicity or Proteasome Inhibition in a Neuronal Cell Line. J. Biol. Chem. 277: 4010-4017 [Abstract] [Full Text]
Aplin, A. E., Hogan, B. P., Tomeu, J., Juliano, R. L. (2002). Cell adhesion differentially regulates the nucleocytoplasmic distribution of active MAP kinases. J. Cell Sci. 115: 2781-2790 [Abstract] [Full Text]
Yang, Z., Madinova, A., Kozai, T., Joch, H., Aebi, U., Luscher, T. F (2002). Felodipine inhibits nuclear translocation of p42/44 mitogen-activated protein kinase and human smooth muscle cell growth. Cardiovasc Res 53: 227-231 [Abstract] [Full Text]
Matsubayashi, Y., Fukuda, M., Nishida, E. (2001). Evidence for Existence of a Nuclear Pore Complex-mediated, Cytosol-independent Pathway of Nuclear Translocation of ERK MAP Kinase in Permeabilized Cells. J. Biol. Chem. 276: 41755-41760 [Abstract] [Full Text]
Villalonga, P., Lopez-Alcala, C., Bosch, M., Chiloeches, A., Rocamora, N., Gil, J., Marais, R., Marshall, C. J., Bachs, O., Agell, N. (2001). Calmodulin Binds to K-Ras, but Not to H- or N-Ras, and Modulates Its Downstream Signaling. Mol. Cell. Biol. 21: 7345-7354 [Abstract] [Full Text]
Masuda, K., Shima, H., Watanabe, M., Kikuchi, K. (2001). MKP-7, a Novel Mitogen-activated Protein Kinase Phosphatase, Functions as a Shuttle Protein. J. Biol. Chem. 276: 39002-39011 [Abstract] [Full Text]
Freedman, J. E. (2001). Thrombin, Thrombomodulin, and Extracellular Signal-Regulated Kinases Regulating Cellular Proliferation. Circ. Res. 88: 651-653 [Full Text]
Pearson, G., Robinson, F., Beers Gibson, T., Xu, B.-e, Karandikar, M., Berman, K., Cobb, M. H. (2001). Mitogen-Activated Protein (MAP) Kinase Pathways: Regulation and Physiological Functions. Endocr. Rev. 22: 153-183 [Abstract] [Full Text]
Furuno, T., Hirashima, N., Onizawa, S., Sagiya, N., Nakanishi, M. (2001). Nuclear Shuttling of Mitogen-Activated Protein (MAP) Kinase (Extracellular Signal-Regulated Kinase (ERK) 2) Was Dynamically Controlled by MAP/ERK Kinase After Antigen Stimulation in RBL-2H3 Cells. J. Immunol. 166: 4416-4421 [Abstract] [Full Text]
Vrana, J. A., Grant, S. (2001). Synergistic induction of apoptosis in human leukemia cells (U937) exposed to bryostatin 1 and the proteasome inhibitor lactacystin involves dysregulation of the PKC/MAPK cascade. Blood 97: 2105-2114 [Abstract] [Full Text]
Morgan, M. A., Dolp, O., Reuter, C. W. M. (2001). Cell-cycle-dependent activation of mitogen-activated protein kinase kinase (MEK-1/2) in myeloid leukemia cell lines and induction of growth inhibition and apoptosis by inhibitors of RAS signaling. Blood 97: 1823-1834 [Abstract] [Full Text]
Volmat, V., Camps, M., Arkinstall, S., Pouyssegur, J., Lenormand, P. (2001). The nucleus, a site for signal termination by sequestration and inactivation of p42/p44 MAP kinases. J. Cell Sci. 114: 3433-3443 [Abstract] [Full Text]
Eblen, S. T., Catling, A. D., Assanah, M. C., Weber, M. J. (2001). Biochemical and Biological Functions of the N-Terminal, Noncatalytic Domain of Extracellular Signal-Regulated Kinase 2. Mol. Cell. Biol. 21: 249-259 [Abstract] [Full Text]
DeFea, K. A., Vaughn, Z. D., O'Bryan, E. M., Nishijima, D., Déry, O., Bunnett, N. W. (2000). The proliferative and antiapoptotic effects of substance P are facilitated by formation of a beta -arrestin-dependent scaffolding complex. Proc. Natl. Acad. Sci. USA 10.1073/pnas.190276697v1 [Abstract] [Full Text]
Tilton, B., Ho, L., Oberlin, E., Loetscher, P., Baleux, F., Clark-Lewis, I., Thelen, M. (2000). Signal Transduction by Cxc Chemokine Receptor 4: Stromal Cell-Derived Factor 1 Stimulates Prolonged Protein Kinase B and Extracellular Signal-Regulated Kinase 2 Activation in T Lymphocytes. JEM 192: 313-324 [Abstract] [Full Text]
Rovida, E., Marra, F., Baccarini, M., Sbarba, P. D. (2000). Constitutive activation of the MAPK pathway mediates v-fes-induced mitogenesis in murine macrophages. Blood 95: 3959-3963 [Abstract] [Full Text]
Mattison, C. P., Ota, I. M. (2000). Two protein tyrosine phosphatases, Ptp2 and Ptp3, modulate the subcellular localization of the Hog1 MAP kinase in yeast. Genes Dev. 14: 1229-1235 [Abstract] [Full Text]
Adachi, M., Fukuda, M., Nishida, E. (2000). Nuclear Export of Map Kinase (ERK) Involves a Map Kinase Kinase (Mek-Dependent) Active Transport Mechanism. JCB 148: 849-856 [Abstract] [Full Text]
Metjian, A., Roll, R. L., Ma, A. D., Abrams, C. S. (1999). Agonists Cause Nuclear Translocation of Phosphatidylinositol 3-Kinase gamma . A Gbeta gamma -DEPENDENT PATHWAY THAT REQUIRES THE p110gamma AMINO TERMINUS. J. Biol. Chem. 274: 27943-27947 [Abstract] [Full Text]
Aubert, J., Dessolin, S., Belmonte, N., Li, M., McKenzie, F. R., Staccini, L., Villageois, P., Barhanin, B., Vernallis, A., Smith, A. G., Ailhaud, G., Dani, C. (1999). Leukemia Inhibitory Factor and Its Receptor Promote Adipocyte Differentiation via the Mitogen-activated Protein Kinase Cascade. J. Biol. Chem. 274: 24965-24972 [Abstract] [Full Text]
Zuniga, A., Torres, J., Ubeda, J., Pulido, R. (1999). Interaction of Mitogen-activated Protein Kinases with the Kinase Interaction Motif of the Tyrosine Phosphatase PTP-SL Provides Substrate Specificity and Retains ERK2 in the Cytoplasm. J. Biol. Chem. 274: 21900-21907 [Abstract] [Full Text]
Shapiro, P. S., Whalen, A. M., Tolwinski, N. S., Wilsbacher, J., Froelich-Ammon, S. J., Garcia, M., Osheroff, N., Ahn, N. G. (1999). Extracellular Signal-Regulated Kinase Activates Topoisomerase IIalpha through a Mechanism Independent of Phosphorylation. Mol. Cell. Biol. 19: 3551-3560 [Abstract] [Full Text]
Reiser, V., Ruis, H., Ammerer, G. (1999). Kinase Activity-dependent Nuclear Export Opposes Stress-induced Nuclear Accumulation and Retention of Hog1 Mitogen-activated Protein Kinase in the Budding Yeast Saccharomyces cerevisiae. Mol. Biol. Cell 10: 1147-1161 [Abstract] [Full Text]
Yan, C., Luo, H., Lee, J.-D., Abe, J.-i., Berk, B. C. (2001). Molecular Cloning of Mouse ERK5/BMK1 Splice Variants and Characterization of ERK5 Functional Domains. J. Biol. Chem. 276: 10870-10878 [Abstract] [Full Text]
Kim, S.-W., Muise, A. M., Lyons, P. J., Ro, H.-S. (2001). Regulation of Adipogenesis by a Transcriptional Repressor That Modulates MAPK Activation. J. Biol. Chem. 276: 10199-10206 [Abstract] [Full Text]
Wolf, I., Rubinfeld, H., Yoon, S., Marmor, G., Hanoch, T., Seger, R. (2001). Involvement of the Activation Loop of ERK in the Detachment from Cytosolic Anchoring. J. Biol. Chem. 276: 24490-24497 [Abstract] [Full Text]
Tarrega, C., Blanco-Aparicio, C., Munoz, J. J., Pulido, R. (2002). Two Clusters of Residues at the Docking Groove of Mitogen-activated Protein Kinases Differentially Mediate Their Functional Interaction with the Tyrosine Phosphatases PTP-SL and STEP. J. Biol. Chem. 277: 2629-2636 [Abstract] [Full Text]
Khan, K. M. F., Falcone, D. J., Kraemer, R. (2002). Nerve Growth Factor Activation of Erk-1 and Erk-2 Induces Matrix Metalloproteinase-9 Expression in Vascular Smooth Muscle Cells. J. Biol. Chem. 277: 2353-2359 [Abstract] [Full Text]
Kim-Kaneyama, J.-r., Nose, K., Shibanuma, M. (2000). Significance of Nuclear Relocalization of ERK1/2 in Reactivation of c-fos Transcription and DNA Synthesis in Senescent Fibroblasts. J. Biol. Chem. 275: 20685-20692 [Abstract] [Full Text]
Cyert, M. S. (2001). Regulation of Nuclear Localization during Signaling. J. Biol. Chem. 276: 20805-20808 [Full Text]
DeFea, K. A., Vaughn, Z. D., O'Bryan, E. M., Nishijima, D., Dery, O., Bunnett, N. W. (2000). The proliferative and antiapoptotic effects of substance P are facilitated by formation of a beta -arrestin-dependent scaffolding complex. Proc. Natl. Acad. Sci. USA 97: 11086-11091 [Abstract] [Full Text]