J. Cell Biol., Volume 142, Number 3, August 10, 1998 665-681

A Membrane Coat Complex Essential for Endosome-to-Golgi Retrograde Transport in Yeast

Matthew N.J. Seaman, J. Michael McCaffery, and Scott D. Emr

Division of Cellular and Molecular Medicine and Howard Hughes Medical Institute, University of California at San Diego, School of Medicine, La Jolla, California 92093-0668

We have recently characterized three yeast gene products (Vps35p, Vps29p, and Vps30p) as candidate components of the sorting machinery required for the endosome-to-Golgi retrieval of the vacuolar protein sorting receptor Vps10p (Seaman, M.N.J., E.G. Marcusson, J.-L. Cereghino, and S.D. Emr. 1997. J. Cell Biol. 137:79-92). By genetic and biochemical means we now show that Vps35p and Vps29p interact and form part of a multimeric membrane-associated complex that also contains Vps26p, Vps17p, and Vps5p. This complex, designated here as the retromer complex, assembles from two distinct subcomplexes comprising (a) Vps35p, Vps29p, and Vps26p; and (b) Vps5p and Vps17p. Density gradient fractionation of Golgi/endosomal/vesicular membranes reveals that Vps35p cofractionates with Vps5p/Vps17p in a vesicle-enriched dense membrane fraction. Furthermore, gel filtration analysis indicates that Vps35p and Vps5p are present on a population of vesicles and tubules slightly larger than COPI/coatomer-coated vesicles. We also show by immunogold EM that Vps5p is localized to discrete regions at the rims of the prevacuolar endosome where vesicles appear to be budding. Size fractionation of cytosolic and recombinant Vps5p reveals that Vps5p can self-assemble in vitro, suggesting that Vps5p may provide the mechanical impetus to drive vesicle formation. Based on these findings we propose a model in which Vps35p/Vps29p/Vps26p function to select cargo for retrieval, and Vps5p/Vps17p assemble onto the membrane to promote vesicle formation. Conservation of the yeast retromer complex components in higher eukaryotes suggests an important general role for this complex in endosome-to-Golgi retrieval.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

• Katz, M. E., Evans, C. J., Heagney, E. E., vanKuyk, P. A., Kelly, J. M., Cheetham, B. F. (2009). Mutations in Genes Encoding Sorting Nexins Alter Production ofIntracellular and Extracellular Proteases in Aspergillus nidulans. Genetics 181: 1239-1247 [Abstract] [Full Text]  
• Anand, V. C., Daboussi, L., Lorenz, T. C., Payne, G. S. (2009). Genome-wide Analysis of AP-3-dependent Protein Transport in Yeast. Mol. Biol. Cell 20: 1592-1604 [Abstract] [Full Text]  
• Rojas, R., van Vlijmen, T., Mardones, G. A., Prabhu, Y., Rojas, A. L., Mohammed, S., Heck, A. J.R., Raposo, G., van der Sluijs, P., Bonifacino, J. S. (2008). Regulation of retromer recruitment to endosomes by sequential action of Rab5 and Rab7. JCB 183: 513-526 [Abstract] [Full Text]  
• Phan, N. Q., Kim, S.-J., Bassham, D. C. (2008). Overexpression of Arabidopsis Sorting Nexin AtSNX2b Inhibits Endocytic Trafficking to the Vacuole. Mol Plant 1: 961-976 [Abstract] [Full Text]  
• Strochlic, T. I., Schmiedekamp, B. C., Lee, J., Katzmann, D. J., Burd, C. G. (2008). Opposing Activities of the Snx3-Retromer Complex and ESCRT Proteins Mediate Regulated Cargo Sorting at a Common Endosome. Mol. Biol. Cell 19: 4694-4706 [Abstract] [Full Text]  
• Morishita, M., Engebrecht, J. (2008). Sorting Signals within the Saccharomyces cerevisiae Sporulation-Specific Dityrosine Transporter, Dtr1p, C Terminus Promote Golgi-to-Prospore Membrane Transport. Eukaryot Cell 7: 1674-1684 [Abstract] [Full Text]  
• Hara, S., Kiyokawa, E., Iemura, S.-i., Natsume, T., Wassmer, T., Cullen, P. J., Hiai, H., Matsuda, M. (2008). The DHR1 Domain of DOCK180 Binds to SNX5 and Regulates Cation-independent Mannose 6-phosphate Receptor Transport. Mol. Biol. Cell 19: 3823-3835 [Abstract] [Full Text]  
• Nakano, K., Yamamoto, T., Kishimoto, T., Noji, T., Tanaka, K. (2008). Protein Kinases Fpk1p and Fpk2p are Novel Regulators of Phospholipid Asymmetry. Mol. Biol. Cell 19: 1783-1797 [Abstract] [Full Text]  
• Soper, J. H., Roy, S., Stieber, A., Lee, E., Wilson, R. B., Trojanowski, J. Q., Burd, C. G., Lee, V. M.-Y. (2008). {alpha}-Synuclein-induced Aggregation of Cytoplasmic Vesicles in Saccharomyces cerevisiae. Mol. Biol. Cell 19: 1093-1103 [Abstract] [Full Text]  
• Yamazaki, M., Shimada, T., Takahashi, H., Tamura, K., Kondo, M., Nishimura, M., Hara-Nishimura, I. (2008). Arabidopsis VPS35, a Retromer Component, is Required for Vacuolar Protein Sorting and Involved in Plant Growth and Leaf Senescence. Plant Cell Physiol 49: 142-156 [Abstract] [Full Text]  
• Korolchuk, V. I., Schutz, M. M., Gomez-Llorente, C., Rocha, J., Lansu, N. R., Collins, S. M., Wairkar, Y. P., Robinson, I. M., O'Kane, C. J. (2007). Drosophila Vps35 function is necessary for normal endocytic trafficking and actin cytoskeleton organisation. J. Cell Sci. 120: 4367-4376 [Abstract] [Full Text]  
• Onishi, M., Iida, M., Koga, T., Yamada, S., Hirata, A., Iwaki, T., Takegawa, K., Fukui, Y., Tachikawa, H. (2007). Schizosaccharomyces pombe Sst4p, a Conserved Vps27/Hrs Homolog, Functions Downstream of Phosphatidylinositol 3-Kinase Pik3p To Mediate Proper Spore Formation. Eukaryot Cell 6: 2343-2353 [Abstract] [Full Text]  
• Seaman, M. N. J. (2007). Identification of a novel conserved sorting motif required for retromer-mediated endosome-to-TGN retrieval. J. Cell Sci. 120: 2378-2389 [Abstract] [Full Text]  
• Bujny, M. V., Popoff, V., Johannes, L., Cullen, P. J. (2007). The retromer component sorting nexin-1 is required for efficient retrograde transport of Shiga toxin from early endosome to the trans Golgi network. J. Cell Sci. 120: 2010-2021 [Abstract] [Full Text]  
• Popoff, V., Mardones, G. A., Tenza, D., Rojas, R., Lamaze, C., Bonifacino, J. S., Raposo, G., Johannes, L. (2007). The retromer complex and clathrin define an early endosomal retrograde exit site. J. Cell Sci. 120: 2022-2031 [Abstract] [Full Text]  
• Strochlic, T. I., Setty, T. G., Sitaram, A., Burd, C. G. (2007). Grd19/Snx3p functions as a cargo-specific adapter for retromer-dependent endocytic recycling. JCB 177: 115-125 [Abstract] [Full Text]  
• Rojas, R., Kametaka, S., Haft, C. R., Bonifacino, J. S. (2007). Interchangeable but Essential Functions of SNX1 and SNX2 in the Association of Retromer with Endosomes and the Trafficking of Mannose 6-Phosphate Receptors. Mol. Cell. Biol. 27: 1112-1124 [Abstract] [Full Text]  
• Kama, R., Robinson, M., Gerst, J. E. (2007). Btn2, a Hook1 Ortholog and Potential Batten Disease-Related Protein, Mediates Late Endosome-Golgi Protein Sorting in Yeast. Mol. Cell. Biol. 27: 605-621 [Abstract] [Full Text]  
• Wassmer, T., Attar, N., Bujny, M. V., Oakley, J., Traer, C. J., Cullen, P. J. (2007). A loss-of-function screen reveals SNX5 and SNX6 as potential components of the mammalian retromer. J. Cell Sci. 120: 45-54 [Abstract] [Full Text]  
• Coudreuse, D., Korswagen, H. C. (2007). The making of Wnt: new insights into Wnt maturation, sorting and secretion. Development 134: 3-12 [Full Text]  
• Shimada, T., Koumoto, Y., Li, L., Yamazaki, M., Kondo, M., Nishimura, M., Hara-Nishimura, I. (2006). AtVPS29, a Putative Component of a Retromer Complex, is Required for the Efficient Sorting of Seed Storage Proteins. Plant Cell Physiol 47: 1187-1194 [Abstract] [Full Text]  
• Foresti, O., daSilva, L. L.P., Denecke, J. (2006). Overexpression of the Arabidopsis Syntaxin PEP12/SYP21 Inhibits Transport from the Prevacuolar Compartment to the Lytic Vacuole in Vivo. Plant Cell 18: 2275-2293 [Abstract] [Full Text]  
• daSilva, L. L.P., Foresti, O., Denecke, J. (2006). Targeting of the Plant Vacuolar Sorting Receptor BP80 Is Dependent on Multiple Sorting Signals in the Cytosolic Tail. Plant Cell 18: 1477-1497 [Abstract] [Full Text]  
• Coudreuse, D. Y. M., Roel, G., Betist, M. C., Destree, O., Korswagen, H. C. (2006). Wnt Gradient Formation Requires Retromer Function in Wnt-Producing Cells. Science 312: 921-924 [Abstract] [Full Text]  
• Iwaki, T., Hosomi, A., Tokudomi, S., Kusunoki, Y., Fujita, Y., Giga-Hama, Y., Tanaka, N., Takegawa, K. (2006). Vacuolar protein sorting receptor in Schizosaccharomyces pombe.. Microbiology 152: 1523-1532 [Abstract] [Full Text]  
• Oliviusson, P., Heinzerling, O., Hillmer, S., Hinz, G., Tse, Y. C., Jiang, L., Robinson, D. G. (2006). Plant Retromer, Localized to the Prevacuolar Compartment and Microvesicles in Arabidopsis, May Interact with Vacuolar Sorting Receptors. Plant Cell 18: 1239-1252 [Abstract] [Full Text]  
• Voronkova, V., Kacherovsky, N., Tachibana, C., Yu, D., Young, E. T. (2006). Snf1-Dependent and Snf1-Independent Pathways of Constitutive ADH2 Expression in Saccharomyces cerevisiae. Genetics 172: 2123-2138 [Abstract] [Full Text]  
• Quenneville, N. R., Chao, T.-Y., McCaffery, J. M., Conibear, E. (2006). Domains within the GARP Subunit Vps54 Confer Separate Functions in Complex Assembly and Early Endosome Recognition. Mol. Biol. Cell 17: 1859-1870 [Abstract] [Full Text]  
• Gullapalli, A., Wolfe, B. L., Griffin, C. T., Magnuson, T., Trejo, J. (2006). An Essential Role for SNX1 in Lysosomal Sorting of Protease-activated Receptor-1: Evidence for Retromer-, Hrs-, and Tsg101-independent Functions of Sorting Nexins. Mol. Biol. Cell 17: 1228-1238 [Abstract] [Full Text]  
• Lindmo, K., Stenmark, H. (2006). Regulation of membrane traffic by phosphoinositide 3-kinases. J. Cell Sci. 119: 605-614 [Abstract] [Full Text]  
• Kruse, K. B., Brodsky, J. L., McCracken, A. A. (2006). Characterization of an ERAD Gene as VPS30/ATG6 Reveals Two Alternative and Functionally Distinct Protein Quality Control Pathways: One for Soluble Z Variant of Human {alpha}-1 Proteinase Inhibitor (A1PiZ) and Another for Aggregates of A1PiZ. Mol. Biol. Cell 17: 203-212 [Abstract] [Full Text]  
• Parrish, W. R., Stefan, C. J., Emr, S. D. (2005). PtdIns(3)P accumulation in triple lipid-phosphatase-deletion mutants triggers lethal hyperactivation of the Rho1p/Pkc1p cell-integrity MAP kinase pathway. J. Cell Sci. 118: 5589-5601 [Abstract] [Full Text]  
• Griffin, C. T., Trejo, J., Magnuson, T. (2005). Genetic evidence for a mammalian retromer complex containing sorting nexins 1 and 2. Proc. Natl. Acad. Sci. USA 102: 15173-15177 [Abstract] [Full Text]  
• Carlton, J. G., Bujny, M. V., Peter, B. J., Oorschot, V. M. J., Rutherford, A., Arkell, R. S., Klumperman, J., McMahon, H. T., Cullen, P. J. (2005). Sorting nexin-2 is associated with tubular elements of the early endosome, but is not essential for retromer-mediated endosome-to-TGN transport. J. Cell Sci. 118: 4527-4539 [Abstract] [Full Text]  
• Wang, D., Guo, M., Liang, Z., Fan, J., Zhu, Z., Zang, J., Zhu, Z., Li, X., Teng, M., Niu, L., Dong, Y., Liu, P. (2005). Crystal Structure of Human Vacuolar Protein Sorting Protein 29 Reveals a Phosphodiesterase/Nuclease-like Fold and Two Protein-Protein Interaction Sites. J. Biol. Chem. 280: 22962-22967 [Abstract] [Full Text]  
• Krsmanovic, T., Pawelec, A., Sydor, T., Kolling, R. (2005). Control of Ste6 Recycling by Ubiquitination in the Early Endocytic Pathway in Yeast. Mol. Biol. Cell 16: 2809-2821 [Abstract] [Full Text]  
• Zhong, Q., Watson, M. J., Lazar, C. S., Hounslow, A. M., Waltho, J. P., Gill, G. N. (2005). Determinants of the Endosomal Localization of Sorting Nexin 1. Mol. Biol. Cell 16: 2049-2057 [Abstract] [Full Text]  
• Nazarian, J., Bouri, K., Hoffman, E. P. (2005). Intracellular expression profiling by laser capture microdissection: three novel components of the neuromuscular junction. Physiol. Genomics 21: 70-80 [Abstract] [Full Text]  
• Johnston, H. D., Foote, C., Santeford, A., Nothwehr, S. F. (2005). Golgi-to-Late Endosome Trafficking of the Yeast Pheromone Processing Enzyme Ste13p Is Regulated by a Phosphorylation Site in its Cytosolic Domain. Mol. Biol. Cell 16: 1456-1468 [Abstract] [Full Text]  
• daSilva, L. L.P., Taylor, J. P., Hadlington, J. L., Hanton, S. L., Snowden, C. J., Fox, S. J., Foresti, O., Brandizzi, F., Denecke, J. (2005). Receptor Salvage from the Prevacuolar Compartment Is Essential for Efficient Vacuolar Protein Targeting. Plant Cell 17: 132-148 [Abstract] [Full Text]  
• Merino-Trigo, A., Kerr, M. C., Houghton, F., Lindberg, A., Mitchell, C., Teasdale, R. D., Gleeson, P. A. (2004). Sorting nexin 5 is localized to a subdomain of the early endosomes and is recruited to the plasma membrane following EGF stimulation. J. Cell Sci. 117: 6413-6424 [Abstract] [Full Text]  
• Saito-Nakano, Y., Yasuda, T., Nakada-Tsukui, K., Leippe, M., Nozaki, T. (2004). Rab5-associated Vacuoles Play a Unique Role in Phagocytosis of the Enteric Protozoan Parasite Entamoeba histolytica. J. Biol. Chem. 279: 49497-49507 [Abstract] [Full Text]  
• Strope, S., Rivi, R., Metzger, T., Manova, K., Lacy, E. (2004). Mouse amnionless, which is required for primitive streak assembly, mediates cell-surface localization and endocytic function of cubilin on visceral endoderm and kidney proximal tubules. Development 131: 4787-4795 [Abstract] [Full Text]  
• Bruinsma, P., Spelbrink, R. G., Nothwehr, S. F. (2004). Retrograde Transport of the Mannosyltransferase Och1p to the Early Golgi Requires a Component of the COG Transport Complex. J. Biol. Chem. 279: 39814-39823 [Abstract] [Full Text]  
• Parrish, W. R., Stefan, C. J., Emr, S. D. (2004). Essential Role for the Myotubularin-related Phosphatase Ymr1p and the Synaptojanin-like Phosphatases Sjl2p and Sjl3p in Regulation of Phosphatidylinositol 3-Phosphate in Yeast. Mol. Biol. Cell 15: 3567-3579 [Abstract] [Full Text]  
• Koga, T., Onishi, M., Nakamura, Y., Hirata, A., Nakamura, T., Shimoda, C., Iwaki, T., Takegawa, K., Fukui, Y. (2004). Sorting nexin homologues are targets of phosphatidylinositol 3-phosphate in sporulation of Schizosaccharomyces pombe. GENES CELLS 9: 561-574 [Abstract] [Full Text]  
• Gullapalli, A., Garrett, T. A., Paing, M. M., Griffin, C. T., Yang, Y., Trejo, J. (2004). A Role for Sorting Nexin 2 in Epidermal Growth Factor Receptor Down-regulation: Evidence for Distinct Functions of Sorting Nexin 1 and 2 in Protein Trafficking. Mol. Biol. Cell 15: 2143-2155 [Abstract] [Full Text]  
• Reggiori, F., Wang, C.-W., Nair, U., Shintani, T., Abeliovich, H., Klionsky, D. J. (2004). Early Stages of the Secretory Pathway, but Not Endosomes, Are Required for Cvt Vesicle and Autophagosome Assembly in Saccharomyces cerevisiae. Mol. Biol. Cell 15: 2189-2204 [Abstract] [Full Text]  
• Seaman, M. N.J. (2004). Cargo-selective endosomal sorting for retrieval to the Golgi requires retromer. JCB 165: 111-122 [Abstract] [Full Text]  
• Arighi, C. N., Hartnell, L. M., Aguilar, R. C., Haft, C. R., Bonifacino, J. S. (2004). Role of the mammalian retromer in sorting of the cation-independent mannose 6-phosphate receptor. JCB 165: 123-133 [Abstract] [Full Text]  
• Ikonomov, O. C., Sbrissa, D., Mlak, K., Deeb, R., Fligger, J., Soans, A., Finley, R. L. Jr., Shisheva, A. (2003). Active PIKfyve Associates with and Promotes the Membrane Attachment of the Late Endosome-to-trans-Golgi Network Transport Factor Rab9 Effector p40. J. Biol. Chem. 278: 50863-50871 [Abstract] [Full Text]  
• DiNitto, J. P., Cronin, T. C., Lambright, D. G. (2003). Membrane Recognition and Targeting by Lipid-Binding Domains. Sci Signal 2003: re16-re16 [Abstract] [Full Text]  
• Vanoosthuyse, V., Tichtinsky, G., Dumas, C., Gaude, T., Cock, J. M. (2003). Interaction of Calmodulin, a Sorting Nexin and Kinase-Associated Protein Phosphatase with the Brassica oleracea S Locus Receptor Kinase. Plant Physiol. 133: 919-929 [Abstract] [Full Text]  
• Pons, V., Hullin-Matsuda, F., Nauze, M., Barbaras, R., Peres, C., Collet, X., Perret, B., Chap, H., Gassama-Diagne, A. (2003). Enterophilin-1, a New Partner of Sorting Nexin 1, Decreases Cell Surface Epidermal Growth Factor Receptor. J. Biol. Chem. 278: 21155-21161 [Abstract] [Full Text]  
• Pimpl, P., Hanton, S. L., Taylor, J. P., Pinto-daSilva, L. L., Denecke, J. (2003). The GTPase ARF1p Controls the Sequence-Specific Vacuolar Sorting Route to the Lytic Vacuole. Plant Cell 15: 1242-1256 [Abstract] [Full Text]  
• Ha, S.-A., Torabinejad, J., DeWald, D. B., Wenk, M. R., Lucast, L., De Camilli, P., Newitt, R. A., Aebersold, R., Nothwehr, S. F. (2003). The Synaptojanin-like Protein Inp53/Sjl3 Functions with Clathrin in a Yeast TGN-to-Endosome Pathway Distinct from the GGA Protein-dependent Pathway. Mol. Biol. Cell 14: 1319-1333 [Abstract] [Full Text]  
• Luo, Z., Gallwitz, D. (2003). Biochemical and Genetic Evidence for the Involvement of Yeast Ypt6-GTPase in Protein Retrieval to Different Golgi Compartments. J. Biol. Chem. 278: 791-799 [Abstract] [Full Text]  
• Deloche, O., Schekman, R. W. (2002). Vps10p Cycles between the TGN and the Late Endosome via the Plasma Membrane in Clathrin Mutants. Mol. Biol. Cell 13: 4296-4307 [Abstract] [Full Text]  
• Burda, P., Padilla, S. M., Sarkar, S., Emr, S. D. (2002). Retromer function in endosome-to-Golgi retrograde transport is regulated by the yeast Vps34 PtdIns 3-kinase. J. Cell Sci. 115: 3889-3900 [Abstract] [Full Text]  
• Schwarz, D. G., Griffin, C. T., Schneider, E. A., Yee, D., Magnuson, T. (2002). Genetic Analysis of Sorting Nexins 1 and 2 Reveals a Redundant and Essential Function in Mice. Mol. Biol. Cell 13: 3588-3600 [Abstract] [Full Text]  
• Seaman, M. N.J., Williams, H. P. (2002). Identification of the Functional Domains of Yeast Sorting Nexins Vps5p and Vps17p. Mol. Biol. Cell 13: 2826-2840 [Abstract] [Full Text]  
• Li, Y., Chin, L.-S., Levey, A. I., Li, L. (2002). Huntingtin-associated Protein 1 Interacts with Hepatocyte Growth Factor-regulated Tyrosine Kinase Substrate and Functions in Endosomal Trafficking. J. Biol. Chem. 277: 28212-28221 [Abstract] [Full Text]  
• Wang, Y., Zhou, Y., Szabo, K., Haft, C. R., Trejo, J. (2002). Down-Regulation of Protease-activated Receptor-1 Is Regulated by Sorting Nexin 1. Mol. Biol. Cell 13: 1965-1976 [Abstract] [Full Text]  
• Zhong, Q., Lazar, C. S., Tronchere, H., Sato, T., Meerloo, T., Yeo, M., Songyang, Z., Emr, S. D., Gill, G. N. (2002). Endosomal localization and function of sorting nexin 1. Proc. Natl. Acad. Sci. USA 99: 6767-6772 [Abstract] [Full Text]  
• McConville, M. J., Mullin, K. A., Ilgoutz, S. C., Teasdale, R. D. (2002). Secretory Pathway of Trypanosomatid Parasites. Microbiol. Mol. Biol. Rev. 66: 122-154 [Abstract] [Full Text]  
• de Figueiredo, P., Doody, A., Polizotto, R. S., Drecktrah, D., Wood, S., Banta, M., Strang, M. S., Brown, W. J. (2001). Inhibition of Transferrin Recycling and Endosome Tubulation by Phospholipase A2 Antagonists. J. Biol. Chem. 276: 47361-47370 [Abstract] [Full Text]  
• Mullins, C., Bonifacino, J. S. (2001). Structural Requirements for Function of Yeast GGAs in Vacuolar Protein Sorting, alpha -Factor Maturation, and Interactions with Clathrin. Mol. Cell. Biol. 21: 7981-7994 [Abstract] [Full Text]  
• Sato, T. K., Overduin, M., Emr, S. D. (2001). Location, Location, Location: Membrane Targeting Directed by PX Domains. Science 294: 1881-1885 [Abstract] [Full Text]  
• Yu, J. W., Lemmon, M. A. (2001). All Phox Homology (PX) Domains from Saccharomyces cerevisiae Specifically Recognize Phosphatidylinositol 3-Phosphate. J. Biol. Chem. 276: 44179-44184 [Abstract] [Full Text]  
• Ha, S.-A., Bunch, J. T., Hama, H., DeWald, D. B., Nothwehr, S. F. (2001). A Novel Mechanism for Localizing Membrane Proteins to Yeast Trans-Golgi Network Requires Function of Synaptojanin-like Protein. Mol. Biol. Cell 12: 3175-3190 [Abstract] [Full Text]  
• Borrelly, G., Boyer, J.-C., Touraine, B., Szponarski, W., Rambier, M., Gibrat, R. (2001). The yeast mutant vps5Delta affected in the recycling of Golgi membrane proteins displays an enhanced vacuolar Mg2+/H+ exchange activity. Proc. Natl. Acad. Sci. USA 10.1073/pnas.161215198v1 [Abstract] [Full Text]  
• Du, L.-L., Novick, P. (2001). Yeast Rab GTPase-activating Protein Gyp1p Localizes to the Golgi Apparatus and Is a Negative Regulator of Ypt1p. Mol. Biol. Cell 12: 1215-1226 [Abstract] [Full Text]  
• Sato, K., Sato, M., Nakano, A. (2001). Rer1p, a Retrieval Receptor for Endoplasmic Reticulum Membrane Proteins, Is Dynamically Localized to the Golgi Apparatus by Coatomer. JCB 152: 935-944 [Abstract] [Full Text]  
• Kranz, A., Kinner, A., Kölling, R. (2001). A Family of Small Coiled-Coil-forming Proteins Functioning at the Late Endosome in Yeast. Mol. Biol. Cell 12: 711-723 [Abstract] [Full Text]  
• Kurten, R., Eddington, A., Chowdhury, P, Smith, R., Davidson, A., Shank, B. (2001). Self-assembly and binding of a sorting nexin to sorting endosomes. J. Cell Sci. 114: 1743-1756 [Abstract]  
• Bensen, E. S., Yeung, B. G., Payne, G. S. (2001). Ric1p and the Ypt6p GTPase Function in a Common Pathway Required for Localization of Trans-Golgi Network Membrane Proteins. Mol. Biol. Cell 12: 13-26 [Abstract] [Full Text]  
• Grote, E., Vlacich, G., Pypaert, M., Novick, P. J. (2000). A snc1 Endocytosis Mutant: Phenotypic Analysis and Suppression by Overproduction of Dihydrosphingosine Phosphate Lyase. Mol. Biol. Cell 11: 4051-4065 [Abstract] [Full Text]  
• Haft, C. R., Sierra, M. d. l. L., Bafford, R., Lesniak, M. A., Barr, V. A., Taylor, S. I. (2000). Human Orthologs of Yeast Vacuolar Protein Sorting Proteins Vps26, 29, and 35: Assembly into Multimeric Complexes. Mol. Biol. Cell 11: 4105-4116 [Abstract] [Full Text]  
• Black, M., Pelham, H. (2000). A Selective Transport Route from Golgi to Late Endosomes that Requires the Yeast GGA Proteins. JCB 151: 587-600 [Abstract] [Full Text]  
• Nothwehr, S. F., Ha, S.-A., Bruinsma, P. (2000). Sorting of Yeast Membrane Proteins into an Endosome-to-Golgi Pathway Involves Direct Interaction of Their Cytosolic Domains with Vps35p. JCB 151: 297-310 [Abstract] [Full Text]  
• Conboy, M. J., Cyert, M. S. (2000). Luv1p/Rki1p/Tcs3p/Vps54p, a Yeast Protein That Localizes to the Late Golgi and Early Endosome, Is Required for Normal Vacuolar Morphology.. Mol. Biol. Cell 11: 2429-2443 [Abstract] [Full Text]  
• Rohn, W., Rouille, Y, Waguri, S, Hoflack, B (2000). Bi-directional trafficking between the trans-Golgi network and the endosomal/lysosomal system. J. Cell Sci. 113: 2093-2101 [Abstract]  
• Luzio, J., Rous, B., Bright, N., Pryor, P., Mullock, B., Piper, R. (2000). Lysosome-endosome fusion and lysosome biogenesis. J. Cell Sci. 113: 1515-1524 [Abstract]  
• Bensen, E. S., Costaguta, G., Payne, G. S. (2000). Synthetic Genetic Interactions With Temperature-Sensitive Clathrin in Saccharomyces cerevisiae: Roles for Synaptojanin-Like Inp53p and Dynamin-Related Vps1p in Clathrin-Dependent Protein Sorting at the trans-Golgi Network. Genetics 154: 83-97 [Abstract] [Full Text]  
• Lewis, M. J., Nichols, B. J., Prescianotto-Baschong, C., Riezman, H., Pelham, H. R. B. (2000). Specific Retrieval of the Exocytic SNARE Snc1p from Early Yeast Endosomes. Mol. Biol. Cell 11: 23-38 [Abstract] [Full Text]  
• Conibear, E., Stevens, T. H. (2000). Vps52p, Vps53p, and Vps54p Form a Novel Multisubunit Complex Required for Protein Sorting at the Yeast Late Golgi. Mol. Biol. Cell 11: 305-323 [Abstract] [Full Text]  
• Chen, C.-Y., Ingram, M. F., Rosal, P. H., Graham, T. R. (1999). Role for Drs2p, a P-Type ATPase and Potential Aminophospholipid Translocase, in Yeast Late Golgi Function. JCB 147: 1223-1236 [Abstract] [Full Text]  
• Spelbrink, R. G., Nothwehr, S. F. (1999). The Yeast GRD20 Gene Is Required for Protein Sorting in the trans-Golgi Network/Endosomal System and for Polarization of the Actin Cytoskeleton. Mol. Biol. Cell 10: 4263-4281 [Abstract] [Full Text]  
• Stephens, D. J., Banting, G. (1999). Direct Interaction of the trans-Golgi Network Membrane Protein, TGN38, with the F-actin Binding Protein, Neurabin. J. Biol. Chem. 274: 30080-30086 [Abstract] [Full Text]  
• Nothwehr, S. F., Bruinsma, P., Strawn, L. A. (1999). Distinct Domains within Vps35p Mediate the Retrieval of Two Different Cargo Proteins from the Yeast Prevacuolar/Endosomal Compartment. Mol. Biol. Cell 10: 875-890 [Abstract] [Full Text]  
• Chin, L.-S., Raynor, M. C., Wei, X., Chen, H.-Q., Li, L. (2001). Hrs Interacts with Sorting Nexin 1 and Regulates Degradation of Epidermal Growth Factor Receptor. J. Biol. Chem. 276: 7069-7078 [Abstract] [Full Text]  
• Phillips, S. A., Barr, V. A., Haft, D. H., Taylor, S. I., Haft, C. R. (2001). Identification and Characterization of SNX15, a Novel Sorting Nexin Involved in Protein Trafficking. J. Biol. Chem. 276: 5074-5084 [Abstract] [Full Text]  
• Parks, W. T., Frank, D. B., Huff, C., Renfrew Haft, C., Martin, J., Meng, X., de Caestecker, M. P., McNally, J. G., Reddi, A., Taylor, S. I., Roberts, A. B., Wang, T., Lechleider, R. J. (2001). Sorting Nexin 6, a Novel SNX, Interacts with the Transforming Growth Factor-beta Family of Receptor Serine-Threonine Kinases. J. Biol. Chem. 276: 19332-19339 [Abstract] [Full Text]  
• Van Valkenburgh, H., Shern, J. F., Sharer, J. D., Zhu, X., Kahn, R. A. (2001). ADP-ribosylation factors (ARFs) and ARF-like 1 (ARL1) Have Both Specific and Shared Effectors. CHARACTERIZING ARL1-BINDING PROTEINS. J. Biol. Chem. 276: 22826-22837 [Abstract] [Full Text]  
• Borrelly, G., Boyer, J.-C., Touraine, B., Szponarski, W., Rambier, M., Gibrat, R. (2001). The yeast mutant vps5Delta affected in the recycling of Golgi membrane proteins displays an enhanced vacuolar Mg2+/H+ exchange activity. Proc. Natl. Acad. Sci. USA 98: 9660-9665 [Abstract] [Full Text]  
• Poon, P. P., Nothwehr, S. F., Singer, R. A., Johnston, G. C. (2001). The Gcs1 and Age2 ArfGAP proteins provide overlapping essential function for transport from the yeast trans-Golgi network. JCB 155: 1239-1250 [Abstract] [Full Text]  
• Jones, S. M., Foreman, S. K., Shank, B. B., Kurten, R. C. (2002). EGF receptor downregulation depends on a trafficking motif in the distal tyrosine kinase domain. Am. J. Physiol. Cell Physiol. 282: C420-C433 [Abstract] [Full Text]  

Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents