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J. Cell Biol.,
Volume 142, Number 3, August 10, 1998 763-774
* Section of Genetics and Development, Cornell University, Ithaca, New York 14853-2703; and Mutations in the Drosophila melanogaster
zw10 gene, which encodes a conserved, essential kinetochore component, abolish the ability of dynein to localize to kinetochores. Several similarities between the
behavior of ZW10 protein and dynein further support a
role for ZW10 in the recruitment of dynein to the kinetochore: (a) in response to bipolar tension across the
chromosomes, both proteins mostly leave the kinetochore at metaphase, when their association with the
spindle becomes apparent; (b) ZW10 and dynein both
bind to functional neocentromeres of structurally acentric minichromosomes; and (c) the localization of both
ZW10 and dynein to the kinetochore is abolished in
cells mutant for the gene rough deal. ZW10's role in the
recruitment of dynein to the kinetochore is likely to be
reasonably direct, because dynamitin, the p50 subunit
of the dynactin complex, interacts with ZW10 in a yeast two-hybrid screen. Since in zw10 mutants no defects in
chromosome behavior are observed before anaphase
onset, our results suggest that dynein at the kinetochore
is essential for neither microtubule capture nor congression to the metaphase plate. Instead, dynein's role
at the kinetochore is more likely to be involved in the
coordination of chromosome separation and/or poleward movement at anaphase onset.
Departments of Genetics and
Cell Biology, University of Minnesota, St. Paul, Minnesota 55108
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