Mitotic Centromere-associated Kinesin Is Important for Anaphase Chromosome Segregation

doi:10.1083/jcb.142.3.787

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© The Rockefeller University Press, 0021-9525/1998//787 $5.00
The Journal of Cell Biology, Volume 142, Number 3, , 1998 787-801

Regular Articles

Mitotic Centromere–associated Kinesin Is Important for Anaphase Chromosome Segregation

Todd Maney, Andrew W. Hunter, Mike Wagenbach, and Linda Wordeman

Department of Physiology and Biophysics, University of Washington School of Medicine, Seattle, Washington 98195

Mitotic centromere–associated kinesin (MCAK) is recruitedto the centromere at prophase and remains centromere associateduntil after telophase. MCAK is a homodimer that is encoded bya single gene and has no associated subunits. A motorless versionof MCAK that binds centromeres but not microtubules disruptschromosome segregation during anaphase. Antisense-induced depletionof MCAK results in the same defect. MCAK overexpression induces centromere-independent bundling and eventual loss of spindlemicrotubule polymer suggesting that centromere-associated bundlingand/or depolymerization activity is required for anaphase.Live cell imaging indicates that MCAK may be required to coordinatethe onset of sister centromere separation.

Key Words: MCAK • centromere • kinesin • mitosis • anaphase

Abbreviations used in this paper: GFP, green fluorescent protein; MCAK, mitotic centromere–associated kinesin.

Address all correspondence to Linda Wordeman, Department ofPhysiology and Biophysics, Box 357290, G424 Health Sciences,University of Washington School of Medicine, Seattle, WA 98195.Tel.: (206) 543-5135. Fax: (206) 685-0619. E-mail: worde{at}u.washington.edu

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