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A correction to this article has been published: J. Cell Biol. 143 (2) 561
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© The Rockefeller University Press, 0021-9525/1998//1023 $5.00
The Journal of Cell Biology, Volume 142, Number 4, , 1998 1023-1034

Articles

Characterization of the p22 Subunit of Dynactin Reveals the Localization of Cytoplasmic Dynein and Dynactin to the Midbody of Dividing Cells



Sher Karki, Bernadette LaMonte, and Erika L.F. Holzbaur

Department of Animal Biology, University of Pennsylvania School of Veterinary Medicine, Philadelphia, Pennsylvania 19104

Dynactin, a multisubunit complex that binds to the microtubule motor cytoplasmic dynein, may provide a link between dynein and its cargo. Many subunits of dynactin have been characterized, elucidating the multifunctional nature of this complex. Using a dynein affinity column, p22, the smallest dynactin subunit, was isolated and microsequenced. The peptide sequences were used to clone a full-length human cDNA. Database searches with the predicted amino acid sequence of p22 indicate that this polypeptide is novel. We have characterized p22 as an integral component of dynactin by biochemical and immunocytochemical methods. Affinity chromatography experiments indicate that p22 binds directly to the p150Glued subunit of dynactin. Immunocytochemistry with antibodies to p22 demonstrates that this polypeptide localizes to punctate cytoplasmic structures and to the centrosome during interphase, and to kinetochores and to spindle poles throughout mitosis. Antibodies to p22, as well as to other dynactin subunits, also revealed a novel localization for dynactin to the cleavage furrow and to the midbodies of dividing cells; cytoplasmic dynein was also localized to these structures. We therefore propose that dynein/dynactin complexes may have a novel function during cytokinesis.

Key Words: microtubules • motor proteins • microtubule-associated proteins (MAPs) • cell division • cytokinesis

Abbreviations used in this paper: Arp1, 45-kD actin-related protein; DIC, dynein intermediate chain; EST, expressed sequence tag.

Address all correspondence to Dr. Erika L.F. Holzbaur, University of Pennsylvania, 143 Rosenthal Building, 3800 Spruce Street, Philadelphia, PA 19104-6046. Tel.: (215) 573-3481. Fax: (215) 898-9923. E-mail: holzbaur{at}vet.upenn.edu


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