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J. Cell Biol.,
Volume 142, Number 5, September 7, 1998 1195-1207
* Istituto Internazionale di Genetica e Biofisica, 80125 Napoli, Italia; and The Nup154 gene of Drosophila encodes a
protein showing similarity with known nucleoporins:
rat Nup155 and yeast Nup170 and Nup157. Hypomorphic mutant alleles of Nup154 affected female and male
fertility, allowing investigation of the gene function in various steps of oogenesis and spermatogenesis.
Nup154 was required in testes for cyst formation, control of spermatocyte proliferation and meiotic progression. In ovaries, Nup154 was essential for egg chamber
development and oocyte growth. In both the male and female germ line, as well as in several other cell types,
the Nup154 protein was detected at the nuclear membrane, but was also present inside the nucleus. Intranuclear localization has not previously been described for
rat Nup155 or yeast Nup170 and Nup157. In mutant egg
chambers the Nup154 protein accumulated in the cytoplasm, while it was only barely detected at the nuclear
envelopes. FG repeats containing nucleoporins detected with mAb414 antibody were also mislocalized to
a certain extent in Nup154 mutant alleles. This suggests
that Nup154 could be required for localizing other nucleoporins within the nuclear pore complex, as previously demonstrated for the yeast Nup170. On the other
hand, no evident defects in lamin localization were observed, indicating that Nup155 mutations did not affect
the overall integrity of the nuclear envelope. However,
ultrastructural analyses revealed that in mutant cells the morphology of the nuclear envelope was altered
near the nuclear pore complexes. Finally, the multiplicity of phenotypes observed in Nup154 mutant alleles
suggests that this gene plays a crucial role in cell physiology.
Dipartimento di Biologia Evoluzionistica,
Universitá di Siena, 53100 Siena, Italia
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