The Yeast V159N Actin Mutant Reveals Roles for Actin Dynamics In Vivo

doi:10.1083/jcb.142.5.1289

This Article

	Full Text
	Full Text (PDF, 617K)
	PPT slides of all figures
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JCB
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Belmont, L. D.
	Articles by Drubin, D. G.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Belmont, L. D.
	Articles by Drubin, D. G.


Social Bookmarking

	What's this?

J. Cell Biol., Volume 142, Number 5, September 7, 1998 1289-1299

The Yeast V159N Actin Mutant Reveals Roles for Actin Dynamics In Vivo

Lisa D. Belmont, and David G. Drubin

Department of Molecular and Cell Biology, University of California, Berkeley, California 94720-3202

Actin with a Val 159 to Asn mutation (V159N) forms actin filaments that depolymerize slowly because of a failure to undergoa conformational change after inorganic phosphate release. Herewe demonstrate that expression of this actin results in reducedactin dynamics in vivo, and we make use of this property to studythe roles of rapid actin filament turnover. Yeast strains expressingthe V159N mutant (act1-159) as their only source of actin havelarger cortical actin patches and more actin cables than wild-typeyeast. Rapid actin dynamics are not essential for cortical actinpatch motility or establishment of cell polarity. However, fluidphase endocytosis is defective in act1-159 strains. act1-159 issynthetically lethal with cofilin and profilin mutants, supportingthe conclusion that mutations in all of these genes impair thepolymerization/ depolymerization cycle. In contrast, act1-159partially suppresses the temperature sensitivity of a tropomyosinmutant, and the loss of cytoplasmic cables seen in fimbrin, Mdm20p,and tropomyosin null mutants, suggesting filament stabilizingfunctions for these actin-binding proteins. Analysis of the cablesin these double-mutant cells supports a role for fimbrin in organizingcytoplasmic cables and for Mdm20p and tropomyosin in excludingcofilin from the cables.

Key words: actin; dynamics; ATP hydrolysis; yeast; cytoskeleton

Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Add to Reddit Reddit Add to Technorati Technorati What's this?

This article has been cited by other articles:

Dighe, S. A., Kozminski, K. G. (2008). Swf1p, a Member of the DHHC-CRD Family of Palmitoyltransferases, Regulates the Actin Cytoskeleton and Polarized Secretion Independently of Its DHHC Motif. Mol. Biol. Cell 19: 4454-4468 [Abstract] [Full Text]
Querin, L., Sanvito, R., Magni, F., Busti, S., Van Dorsselaer, A., Alberghina, L., Vanoni, M. (2008). Proteomic Analysis of a Nutritional Shift-up in Saccharomyces cerevisiae Identifies Gvp36 as a BAR-containing Protein Involved in Vesicular Traffic and Nutritional Adaptation. J. Biol. Chem. 283: 4730-4743 [Abstract] [Full Text]
Okreglak, V., Drubin, D. G. (2007). Cofilin recruitment and function during actin-mediated endocytosis dictated by actin nucleotide state. JCB 178: 1251-1264 [Abstract] [Full Text]
Veerman, E. C. I., Valentijn-Benz, M., Nazmi, K., Ruissen, A. L. A., Walgreen-Weterings, E., van Marle, J., Doust, A. B., van't Hof, W., Bolscher, J. G. M., Amerongen, A. V. N. (2007). Energy Depletion Protects Candida albicans against Antimicrobial Peptides by Rigidifying Its Cell Membrane. J. Biol. Chem. 282: 18831-18841 [Abstract] [Full Text]
Petalcorin, M. I. R., Galkin, V. E., Yu, X., Egelman, E. H., Boulton, S. J. (2007). Stabilization of RAD-51-DNA filaments via an interaction domain in Caenorhabditis elegans BRCA2. Proc. Natl. Acad. Sci. USA 104: 8299-8304 [Abstract] [Full Text]
Toshima, J., Toshima, J. Y., Duncan, M. C., Cope, M. J. T.V., Sun, Y., Martin, A. C., Anderson, S., Yates, J. R. III, Mizuno, K., Drubin, D. G. (2007). Negative Regulation of Yeast Eps15-like Arp2/3 Complex Activator, Pan1p, by the Hip1R-related Protein, Sla2p, during Endocytosis. Mol. Biol. Cell 18: 658-668 [Abstract] [Full Text]
Haarer, B., Viggiano, S., Hibbs, M. A., Troyanskaya, O. G., Amberg, D. C. (2007). Modeling complex genetic interactions in a simple eukaryotic genome: actin displays a rich spectrum of complex haploinsufficiencies. Genes Dev. 21: 148-159 [Abstract] [Full Text]
Moseley, J. B., Goode, B. L. (2006). The Yeast Actin Cytoskeleton: from Cellular Function to Biochemical Mechanism. Microbiol. Mol. Biol. Rev. 70: 605-645 [Abstract] [Full Text]
Stirling, F. R., Evans, T. J. (2006). Effects of the type III secreted pseudomonal toxin ExoS in the yeast Saccharomyces cerevisiae.. Microbiology 152: 2273-2285 [Abstract] [Full Text]
Yoshiuchi, S., Yamamoto, T., Sakane, H., Kadota, J., Mochida, J., Asaka, M., Tanaka, K. (2006). Identification of Novel Mutations in ACT1 and SLA2 That Suppress the Actin-Cable-Overproducing Phenotype Caused by Overexpression of a Dominant Active Form of Bni1p in Saccharomyces cerevisiae. Genetics 173: 527-539 [Abstract] [Full Text]
Reggiori, F., Monastyrska, I., Shintani, T., Klionsky, D. J. (2005). The Actin Cytoskeleton Is Required for Selective Types of Autophagy, but Not Nonspecific Autophagy, in the Yeast Saccharomyces cerevisiae. Mol. Biol. Cell 16: 5843-5856 [Abstract] [Full Text]
Clark, S. W., Rose, M. D. (2005). Alanine Scanning of Arp1 Delineates a Putative Binding Site for Jnm1/Dynamitin and Nip100/p150Glued. Mol. Biol. Cell 16: 3999-4012 [Abstract] [Full Text]
Wen, K.-K., Rubenstein, P. A. (2005). Acceleration of Yeast Actin Polymerization by Yeast Arp2/3 Complex Does Not Require an Arp2/3-activating Protein. J. Biol. Chem. 280: 24168-24174 [Abstract] [Full Text]
Martin, A. C., Xu, X.-P., Rouiller, I., Kaksonen, M., Sun, Y., Belmont, L., Volkmann, N., Hanein, D., Welch, M., Drubin, D. G. (2005). Effects of Arp2 and Arp3 nucleotide-binding pocket mutations on Arp2/3 complex function. JCB 168: 315-328 [Abstract] [Full Text]
Bryan, K. E., Rubenstein, P. A. (2005). An Intermediate Form of ADP-F-actin. J. Biol. Chem. 280: 1696-1703 [Abstract] [Full Text]
Yu, X., Cai, M. (2004). The yeast dynamin-related GTPase Vps1p functions in the organization of the actin cytoskeleton via interaction with Sla1p. J. Cell Sci. 117: 3839-3853 [Abstract] [Full Text]
Cox, K. H., Tate, J. J., Cooper, T. G. (2004). Actin Cytoskeleton Is Required For Nuclear Accumulation of Gln3 in Response to Nitrogen Limitation but Not Rapamycin Treatment in Saccharomyces cerevisiae. J. Biol. Chem. 279: 19294-19301 [Abstract] [Full Text]
Gourlay, C. W., Carpp, L. N., Timpson, P., Winder, S. J., Ayscough, K. R. (2004). A role for the actin cytoskeleton in cell death and aging in yeast. JCB 164: 803-809 [Abstract] [Full Text]
Singer, J. M., Shaw, J. M. (2003). Mdm20 protein functions with Nat3 protein to acetylate Tpm1 protein and regulate tropomyosin-actin interactions in budding yeast. Proc. Natl. Acad. Sci. USA 100: 7644-7649 [Abstract] [Full Text]
Bernstein, B. W., Bamburg, J. R. (2003). Actin-ATP Hydrolysis Is a Major Energy Drain for Neurons. J. Neurosci. 23: 1-6 [Abstract] [Full Text]
Posern, G., Sotiropoulos, A., Treisman, R. (2002). Mutant Actins Demonstrate a Role for Unpolymerized Actin in Control of Transcription by Serum Response Factor. Mol. Biol. Cell 13: 4167-4178 [Abstract] [Full Text]
Idrissi, F.-Z., Wolf, B. L., Geli, M. I. (2002). Cofilin, But Not Profilin, Is Required for Myosin-I-Induced Actin Polymerization and the Endocytic Uptake in Yeast. Mol. Biol. Cell 13: 4074-4087 [Abstract] [Full Text]
Kaminska, J., Gajewska, B., Hopper, A. K., Zoladek, T. (2002). Rsp5p, a New Link between the Actin Cytoskeleton and Endocytosis in the Yeast Saccharomyces cerevisiae. Mol. Cell. Biol. 22: 6946-6948 [Abstract] [Full Text]
Hendron, E., Patel, P., Hausenfluke, M., Gamper, N., Shapiro, M. S., Booth, R. E., Stockand, J. D. (2002). Identification of Cytoplasmic Domains within the Epithelial Na+ Channel Reactive at the Plasma Membrane. J. Biol. Chem. 277: 34480-34488 [Abstract] [Full Text]
Warren, D. T., Andrews, P. D., Gourlay, C. W., Ayscough, K. R. (2002). Sla1p couples the yeast endocytic machinery to proteins regulating actin dynamics. J. Cell Sci. 115: 1703-1715 [Abstract] [Full Text]
Ono, S., Ono, K. (2002). Tropomyosin inhibits ADF/cofilin-dependent actin filament dynamics. JCB 156: 1065-1076 [Abstract] [Full Text]
Yang, H.-C., Pon, L. A. (2002). Actin cable dynamics in budding yeast. Proc. Natl. Acad. Sci. USA 99: 751-756 [Abstract] [Full Text]
Boldogh, I. R., Yang, H.-C., Nowakowski, W. D., Karmon, S. L., Hays, L. G., Yates, J. R. III, Pon, L. A. (2001). Arp2/3 complex and actin dynamics are required for actin-based mitochondrial motility in yeast. Proc. Natl. Acad. Sci. USA 98: 3162-3167 [Abstract] [Full Text]
Cheeseman, I. M., Enquist-Newman, M., Muller-Reichert, T., Drubin, D. G., Barnes, G. (2001). Mitotic Spindle Integrity and Kinetochore Function Linked by the Duo1p/Dam1p Complex. JCB 152: 197-212 [Abstract] [Full Text]
Smith, M., Swamy, S., Pon, L. (2001). The life cycle of actin patches in mating yeast. J. Cell Sci. 114: 1505-1513 [Abstract]
Singer, J. M., Hermann, G. J., Shaw, J. M. (2000). Suppressors of mdm20 in Yeast Identify New Alleles of ACT1 and TPM1 Predicted to Enhance Actin-Tropomyosin Interactions. Genetics 156: 523-534 [Abstract] [Full Text]
Wolven, A. K., Belmont, L. D., Mahoney, N. M., Almo, S. C., Drubin, D. G. (2000). In Vivo Importance of Actin Nucleotide Exchange Catalyzed by Profilin. JCB 150: 895-904 [Abstract] [Full Text]
Andrews, P., Stark, M. (2000). Dynamic, Rho1p-dependent localization of Pkc1p to sites of polarized growth. J. Cell Sci. 113: 2685-2693 [Abstract]
Pruyne, D, Bretscher, A (2000). Polarization of cell growth in yeast. J. Cell Sci. 113: 571-585 [Abstract]
Kozminski, K. G., Chen, A. J., Rodal, A. A., Drubin, D. G. (2000). Functions and Functional Domains of the GTPase Cdc42p. Mol. Biol. Cell 11: 339-354 [Abstract] [Full Text]
Engqvist-Goldstein, A. E.Y., Kessels, M. M., Chopra, V. S., Hayden, M. R., Drubin, D. G. (1999). An Actin-binding Protein of the Sla2/Huntingtin Interacting Protein 1 Family Is a Novel Component of Clathrin-coated Pits and Vesicles. JCB 147: 1503-1518 [Abstract] [Full Text]
Morrell, J. L., Morphew, M., Gould, K. L. (1999). A Mutant of Arp2p Causes Partial Disassembly of the Arp2/3 Complex and Loss of Cortical Actin Function in Fission Yeast. Mol. Biol. Cell 10: 4201-4215 [Abstract] [Full Text]
Rodal, A. A., Tetreault, J. W., Lappalainen, P., Drubin, D. G., Amberg, D. C. (1999). Aip1p Interacts with Cofilin to Disassemble Actin Filaments. JCB 145: 1251-1264 [Abstract] [Full Text]
Ayscough, K. R., Eby, J. J., Lila, T., Dewar, H., Kozminski, K. G., Drubin, D. G. (1999). Sla1p Is a Functionally Modular Component of the Yeast Cortical Actin Cytoskeleton Required for Correct Localization of Both Rho1p-GTPase and Sla2p, a Protein with Talin Homology. Mol. Biol. Cell 10: 1061-1075 [Abstract] [Full Text]
Goode, B. L., Wong, J. J., Butty, A.-C., Peter, M., McCormack, A. L., Yates, J. R., Drubin, D. G., Barnes, G. (1999). Coronin Promotes the Rapid Assembly and Cross-linking of Actin Filaments and May Link the Actin and Microtubule Cytoskeletons in Yeast. JCB 144: 83-98 [Abstract] [Full Text]
Belmont, L. D., Orlova, A., Drubin, D. G., Egelman, E. H. (1999). A change in actin conformation associated with filament instability after Pi release. Proc. Natl. Acad. Sci. USA 96: 29-34 [Abstract] [Full Text]
Belmont, L., Patterson, G., Drubin, D. (1999). New actin mutants allow further characterization of the nucleotide binding cleft and drug binding sites. J. Cell Sci. 112: 1325-1336 [Abstract]
Pruyne, D. W., Schott, D. H., Bretscher, A. (1998). Tropomyosin-containing Actin Cables Direct the Myo2p-dependent Polarized Delivery of Secretory Vesicles in Budding Yeast. JCB 143: 1931-1945 [Abstract] [Full Text]